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Bilal Momin,Snehasis Chakraborty,,Uday Annapure 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.10
Arginase, an intracellular enzyme produced by Bacillus licheniformis (NRS-1264) is effectively used as a drug in the treatment of arginine-dependent cancers, and it is essential for controlling acute neurological disorders. We investigated the effect of various cell disruption methods for maximizing the extraction of intracellular arginase from mutant Bacillus licheniformis (M09), followed by comparing optimization methods, one factor at a time (OFAT), evolutionary operation (EVOP) and response surface method (RSM). Ultrasonication for 2-5min having a suspension volume in the range of 12-20mL at a radio frequency power between 30-70W appeared to be the most effective extraction technique for arginase. The arginase yield decreased in the range of 50-70W of RF power/16-20mL suspension volume and 4-5min sonication time. EVOP predicted a maximum arginase extraction of 3,910IU·L1 at 2min sonication having 16mL suspension volume at 30W RF power. However, response surface optimization suggested an optimized condition of 3min sonication having 14.5mL suspension volume at 35W RF power in which the maximum arginase activity in the medium was 3,600IU·L1.
Rahman, Ebeydulla,Momin, Ali,Zhao, Liang,Guo, Xiaoxuan,Xu, Duoyuan,Zhou, Feng,Ji, Baoping 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
This study aims to conduct a detailed investigation on four cultivars grown in northwest China, concentrating on the analysis of the bioactive contents, nutrients, heavy metal concentrations, and pesticide residue contents. Those Chinese jujubes consist of 51.99-71.75% edible part, 82.35-89.63% carbohydrates, 4.43-6.01% protein, 0.48-0.63% lipid, 2.80-4.80% polysaccharide, 45.64-88.97 mg/100 g ascorbic acid, 132.16-196.58 mg/100 g phenolics and 101.17-132.04 mg/100 g flavonoids in dry matter. In those four Chinese jujube cultivars, sulfur amino acids are the first limiting amino acids for adults, and aromatic amino acids are for children. The amount of heavy metal and pesticide residue concentrations in those jujubes was way below the limit. All four cultivars were found to have different nutritional values except for the carbohydrates; they had higher rates of carbohydrates and polysaccharide than those previously reported ones from Eastern China; and they are a better source for carbohydrates, vitamin C and functional amino acids.
Kim, Taeho,Momin, Eric,Choi, Jonghoon,Yuan, Kristy,Zaidi, Hasan,Kim, Jaeyun,Park, Mihyun,Lee, Nohyun,McMahon, Michael T.,Quinones-Hinojosa, Alfredo,Bulte, Jeff W. M.,Hyeon, Taeghwan,Gilad, Assaf A. American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.9
<P/><P>Mesoporous silica-coated hollow manganese oxide (HMnO@mSiO<SUB>2</SUB>) nanoparticles were developed as a novel <I>T</I><SUB>1</SUB> magnetic resonance imaging (MRI) contrast agent. We hypothesized that the mesoporous structure of the nanoparticle shell enables optimal access of water molecules to the magnetic core, and consequently, an effective longitudinal (<I>R</I><SUB>1</SUB>) relaxation enhancement of water protons, which value was measured to be 0.99 (mM<SUP>−1</SUP>s<SUP>−1</SUP>) at 11.7 T. Adipose-derived mesenchymal stem cells (MSCs) were efficiently labeled using electroporation, with much shorter <I>T</I><SUB>1</SUB> values as compared to direct incubation without electroporation, which was also evidenced by signal enhancement on <I>T</I><SUB>1</SUB>-weighted MR images in vitro. Intracranial grafting of HMnO@mSiO<SUB>2</SUB>-labeled MSCs enabled serial MR monitoring of cell transplants over 14 days. These novel nanoparticles may extend the arsenal of currently available nanoparticle MR contrast agents by providing positive contrast on <I>T</I><SUB>1</SUB>-weighted images at high magnetic field strengths.</P>
Ebeydulla Rahman,Ali Momin,Liang Zhao,Xiaoxuan Guo,Duoyuan Xu,Feng Zhou,Baoping Ji 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
This study aims to conduct a detailed investigation on four cultivars grown in northwest China, concentrating on the analysis of the bioactive contents, nutrients, heavy metal concentrations, and pesticide residue contents. Those Chinese jujubes consist of 51.99–71.75% edible part, 82.35–89.63% carbohydrates, 4.43–6.01% protein, 0.48–0.63% lipid, 2.80–4.80% polysaccharide, 45.64–88.97 mg/100 g ascorbic acid, 132.16–196.58 mg/ 100 g phenolics and 101.17–132.04 mg/100 g flavonoids in dry matter. In those four Chinese jujube cultivars, sulfur amino acids are the first limiting amino acids for adults, and aromatic amino acids are for children. The amount of heavy metal and pesticide residue concentrations in those jujubes was way below the limit. All four cultivars were found to have different nutritional values except for the carbohydrates; they had higher rates of carbohydrates and polysaccharide than those previously reported ones from Eastern China; and they are a better source for carbohydrates, vitamin C and functional amino acids.
Hossain, M.I.,Momin, M.M.,Fakhrul Islam, K.M.,Quader, M.N.,Miah, G. The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.3
It is imperious to know the reproductive parameters of the subsisting swine breed for profitable farming in Bangladesh. This study was designed to compare the reproductive parameters of sows at hilly areas between the local and crossbred at farm and backyard level. A total of 116 breedable sows were included. The data on age at puberty, litter size, birth weight, weaning weight, gestation length, interval between farrowing and number of piglets born per sow in local and crossbred sows were determined and direct questionnaire method was used for data collection. The present study revealed that age at puberty between farm and backyard system of crossbred sows was $8.88{\pm}0.153$ and $9.85{\pm}0.221$ months, respectively. The farrowing interval was shorter in crossbred sows which were kept in farming system rather than backyard system. The interval between farrowing and onset of estrus was shorter in crossbred sows. The gestation length was almost similar between backyard and farm condition. The number of litter size per sow per farrowing was $8.45{\pm}0.41$ in crossbred and $7.17{\pm}0.250$ in local pigs at backyard system. The birth weight and weaning weight were more in farm conditions than backyard system. The age at puberty in local breed was lower than the crossbred and litter size was $8.45{\pm}0.407$ in crossbred which was higher than local breed $7.17{\pm}0.250$ in backyard system. These results suggest that reproductive parameters of local sow need to be improved for better production and crossbred sows should be reared for obtaining desired productivity.
Small RNAs control sodium channel expression, nociceptor excitability, and pain thresholds.
Zhao, Jing,Lee, Man-Cheung,Momin, Ali,Cendan, Cruz-Miguel,Shepherd, Samuel T,Baker, Mark D,Asante, Curtis,Bee, Lucy,Bethry, Audrey,Perkins, James R,Nassar, Mohammed A,Abrahamsen, Bjarke,Dickenson, Ant The Society 2010 The Journal of neuroscience Vol.30 No.32
<P>To examine the role of small RNAs in peripheral pain pathways, we deleted the enzyme Dicer in mouse postmitotic damage-sensing neurons. We used a Nav1.8-Cre mouse to target those nociceptors important for inflammatory pain. The conditional null mice were healthy with a normal number of sensory neurons and normal acute pain thresholds. Behavioral studies showed that inflammatory pain was attenuated or abolished. Inflammatory mediators failed to enhance excitability of Nav1.8+ sensory neurons from null mutant mice. Acute noxious input into the dorsal horn of the spinal cord was apparently normal, but the increased input associated with inflammatory pain measured using c-Fos staining was diminished. Microarray and quantitative real-time reverse-transcription PCR (qRT-PCR) analysis showed that Dicer deletion lead to the upregulation of many broadly expressed mRNA transcripts in dorsal root ganglia. By contrast, nociceptor-associated mRNA transcripts (e.g., Nav1.8, P2xr3, and Runx-1) were downregulated, resulting in lower levels of protein and functional expression. qRT-PCR analysis also showed lowered levels of expression of nociceptor-specific pre-mRNA transcripts. MicroRNA microarray and deep sequencing identified known and novel nociceptor microRNAs in mouse Nav1.8+ sensory neurons that may regulate nociceptor gene expression.</P>
M.I. Hossain,M.M. Momin,K.M. Fakhrul Islam,M.N. Quader,G. Miah 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.3
It is imperious to know the reproductive parameters of the subsisting swine breed for profitable farming in Bangladesh. This study was designed to compare the reproductive parameters of sows at hilly areas between the local and crossbred at farm and backyard level. A total of 116 breedable sows were included. The data on age at puberty, litter size, birth weight, weaning weight, gestation length, interval between farrowing and number of piglets born per sow in local and crossbred sows were determined and direct questionnaire method was used for data collection. The present study revealed that age at puberty between farm and backyard system of crossbred sows was 8.88±0.153 and 9.85±0.221 months, respectively. The farrowing interval was shorter in crossbred sows which were kept in farming system rather than backyard system. The interval between farrowing and onset of estrus was shorter in crossbred sows. The gestation length was almost similar between backyard and farm condition. The number of litter size per sow per farrowing was 8.45±0.41 in crossbred and 7.17±0.250 in local pigs at backyard system. The birth weight and weaning weight were more in farm conditions than backyard system. The age at puberty in local breed was lower than the crossbred and litter size was 8.45±0.407 in crossbred which was higher than local breed 7.17±0.250 in backyard system. These results suggest that reproductive parameters of local sow need to be improved for better production and crossbred sows should be reared for obtaining desired productivity.