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Proximity Proteolysis Reaction as a Universal Platform of Molecular Sensing Methods
Minji GWON,Hyeon Ji PARK,Yuseon KIM,Heeju JANG,Tae Hyeon YOO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Proteins, and small molecules either produced by or introduced into organisms are important indicators for evaluation of the biological systems. Various methods have been developed to assay them in quantitative ways. However, they were designed based on properties of each type of molecule, which inevitably results in development of quite diverse technologies. Here, we present proximity proteolysis reaction (PPR)-based homogeneous assay methods for proteins, antibodies and small molecules. Binders were devised to recognize molecules of interest and induce colocalization of protease and zymogen at an analyte. Physical closeness between two proteins induce PPR, resulting in production of detectable signal. Single-stranded DNAs were utilized as linkers, which allow tethering of enzymes and binders by simple mixing. The application of PPR to different types of analytes needed minimal work. The assays are conducted in a one step at constant temperature, and colorimetric signals were generated in 1 h. In addition, despite the signal based on absorbance, the sensitivities of the assays were below dozens of picomolar concentrations of analytes. We have demonstrated that the PPR platform can be used to develop simple and sensitive assay methods for diverse molecules.
Gwon, Minji,Lee, Y. U.,Wu, J. W.,Nam, Dahyun,Cheong, Hyeonsik,Kim, Dong-Wook American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.11
<P>In this study, we observed that the photoluminescence (PL) intensity of ZnO/Ag nanogratings was significantly enhanced compared with that of a planar counterpart under illumination of both transverse magnetic (TM) and transverse electric (TE)-mode light. In the TM mode, angle-resolved reflectance spectra exhibited dispersive dips, indicating cavity resonance as well as grating-coupled surface plasmon polariton (SPP) excitation. In the TE mode, cavity resonance only was allowed, and broad dips appeared in the reflectance spectra. Strong optical field confinement in the ZnO layers, with the help of SPP and cavity modes, facilitated polarization-insensitive PL enhancement. Optical simulation results were in good agreement with the experimental results, supporting the suggested scenario.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-11/am5014024/production/images/medium/am-2014-014024_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5014024'>ACS Electronic Supporting Info</A></P>
Development of the Hybrid Conjugated Polymer Solar Cell Based on GaN Quantum Dots
Kim, Minji,Shin, Min Jeong,Gwon, Dongoh,Ahn, Hyung Soo,Yi, Sam Nyung,Kim, Pan Seok,Yoon, Sung Cheol,Lee, Changjin,Park, Jungsik,Shin, Keesam,Ha, Dong Han,Sawaki, Nobuhiko IOP Publishing 2013 Japanese journal of applied physics Vol.52 No.1