Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Wang, Xue-Mei,Cui, Jiu-Wei,Li, Wei,Cai, Lu,Song, Wei,Wang, Guan-Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

The COPS3 gene has stimulating effect on cell proliferation and progression of osteosarcomas and related cells. However, the features of COPS3 and its potential application as a therapeutic target in other cancers has not yet been studied. In this study, therefore, the effect of COPS3 silencing via COPS3 siRNA on lung cancer cell proliferation was examined. Expression levels of COPS3 gene in COPS3 siRNA infected cells and control siRNA infected cells were compared with real time PCR and Western blot analysis. Cell proliferation levels were comprehensively analyzed by MTT, BrdU incorporationy, and colony formation assays. For mechanistic assessment the effects of COPS3 silencing on cell cycle and apoptosis were analyzed using flow cytometry. Results showed that successful silencing of the COPS3 gene at both translational and transcriptional levels significantly reduced the proliferation and colony formation by lung cancer cells (p<0.01). Flow cytometry showed cell cycle arrest in the G0/G1 phase after COPS3 silencing, and more importantly, apoptosis was induced as a result of COPS3 knockdown, which negatively affected cell survival. Therefore, these results provide another piece of important evidence that the COPS3 gene expressed in lung cancer cells may play a critical role in stimulating proliferation. Down-regulation of COPS3 could significantly inhibit lung cancer cell growth, which was most likely mediated via induction of cell cycle arrest in G0/G1 phase and apoptosis.

Review of the Macrophya formosana group (Hymenoptera: Tenthredinidae) with descriptions of two new species

Meng-Meng LIU,Ze-Jian LI,Mei-Cai WEI 한국곤충학회 2019 Entomological Research Vol.49 No.5

The Macrophya formosana group is reviewed and six species are recognized from Europe and East Asia, among them two new species, M. brevispuralis Li, Liu & Wei sp. nov. and M. pseudoformosana Li, Liu &Wei sp. nov. from China, and four known species, M. crassula (Klug 1817), M. dolichogaster Wei & Ma, 1997, M. formosana Rohwer 1916 and M. liukiuana Takeuchi 1926. A key to all known species of the Macrophya formosana group is provided.

Expression and characterization of RNA-dependent RNA polymerase of Ectropis obliqua virus

( Mei Juan Lin ),( Shan Ye ),( Yi Xiong ),( Da Wei Cai ),( Jia Min Zhang ),( Yuan Yang Hu ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.4

Replication of positive-strand RNA virus is mediated by a virus- encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated RNA synthesis in a primer- and poly (A)-dependent manner in vitro. Furthermore, the effect of primer concentration, temperature, metal ions (Mg2+, Mn2+, and K+) on enzymatic activity were determined. Our study represented a first step towards understanding the mechanism of EoV replication. [BMB reports 2010; 43(4): 284-290]

Photocatalytic CO2 Reduction over g-C3N4 Based Materials

Wei-Qin Cai,Feng-Jun Zhang,Cui Kong,Chun-Mei Kai,Won-Chun Oh 한국재료학회 2020 한국재료학회지 Vol.30 No.11

Reducing CO2 into high value fuels and chemicals is considered a great challenge in the 21st century. Efficiently activating CO2 will lead to an important way to utilize it as a resource. This article reviews the latest progress of g-C3N4 based catalysts for CO2 reduction. The different synthetic methods of g-C3N4 are briefly discussed. Article mainly introduces methods of g-C3N4 shape control, element doping, and use of oxide compounds to modify g-C3N4. Modified g-C3N4 has more reactive sites, which can significantly reduce the probability of photogenerated electron hole recombination and improve the performance of photocatalytic CO2 reduction. Considering the literature, the hydrothermal method is widely used because of its simple equipment and process and easy control of reaction conditions. It is foreseeable that hydrothermal technology will continue to innovate and usher in a new period of development. Finally, the prospect of a future reduction of CO2 by g-C3N4-based catalysts is predicted.

Monoterpene esters and aporphine alkaloids from Illigera aromatica with inhibitory effects against cholinesterase and NO production in LPS-stimulated RAW264.7 macrophages

Jian-Wei Dong,Le Cai,Xue-Jiao Li,Jia-Peng Wang,Rui-Feng Mei,Zhong-Tao Ding 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.12

Three new monoterpene phenylpropionic acidesters, illigerates A–C (1–3), and one new aporphinealkaloid, illigeranine (4), as well as four known ones,actinodaphnine (5), nordicentrine (6), 8-hydroxy carvacrol(7), and 3-hydroxy-a,4-dimethyl styrene (8), were isolatedfrom the tubers of Illigera aromatica. The structures of 1–4were identified by HRESIMS, 1D and 2D NMR, andelectronic circular dichroism spectra. Compound 1 potentlyinhibited NO production in LPS-stimulated RAW264.7cells with an IC50 value of 18.71 ± 0.85 lM; compound 1,3, and 4 showed moderate butyrylcholinesterase inhibitoryactivities with the IC50 values of 46.86 ± 0.65,53.51 ± 0.71, and 31.62 ± 1.15 lM, respectively. Compound4 showed weak AChE inhibitory activity with anIC50 value of 81.69 ± 2.07 lM, and compounds 5 and 6possessed moderate AChE inhibitory activities with theIC50 values of 47.74 ± 1.66 and 40.28 ± 2.73 lM,respectively. This paper provides a chemical structure andbioactive foundation for using I. aromatica as an herbalmedicine.

Identification of Cisplatin-Resistance Associated Genes through Proteomic Analysis of Human Ovarian Cancer Cells and a Cisplatin-resistant Subline

Zhou, Jing,Wei, Yue-Hua,Liao, Mei-Yan,Xiong, Yan,Li, Jie-Lan,Cai, Hong-Bing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Chemoresistance to cancer therapy is a major obstacle to the effective treatment of human cancers with cisplatin (DDP), but the mechanisms of cisplatin-resistance are not clear. In this study, we established a cisplatin-resistant human ovarian cancer cell line (COC1/DDP) and identified differentially expressed proteins related to cisplatin resistance. The proteomic expression profiles in COC1 before and after DDP treatment were examined using 2-dimensional electrophoresis technology. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and high performance liquid chromatography-electrospray tandem MS (NanoUPLC-ESI-MS/MS). 5 protein spots, for cytokeratin 9, keratin 1, deoxyuridine triphosphatase (dUTPase), aarF domain containing kinase 4 (ADCK 4) and cofilin1, were identified to be significantly changed in COC1/DDP compared with its parental cells. The expression of these five proteins was further validated by quantitative PCR and Western blotting, confirming the results of proteomic analysis. Further research on these proteins may help to identify novel resistant biomarkers or reveal the mechanism of cisplatin-resistance in human ovarian cancers.

Randomized Control Study of Nedaplatin or Cisplatin Concomitant with Other Chemotherapy in the Treatment of Advanced Non-small Cell Lung Cancer

Li, Chun-Hong,Liu, Mei-Yan,Liu, Wei,Li, Dan-Dan,Cai, Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: To observe the short-term efficacy, long-term survival time and adverse responses with nedaplatin (NDP) or cisplatin (DDP) concomitant with other chemotherapy in treating non-small cell lung cancer. Materials and Methods: A retrospective, randomized, control study was conducted, in which 619 NSCLC patients in phases III and IV who were initially treated and re-treated were randomly divided into an NDP group (n=294) and a DDP group (n=325), the latter being regarded as controls. Chemotherapeutic protocols (CP/DP/GP/NP/TP) containing NDP or DDP were given to both groups. Patients in both groups were further divided to evaluate the clinical efficacies according to initial and re-treatment stage, pathological pattern, type of combined chemotherapeutic protocols, tumor stage and surgery. Results: The overall response rate (ORR) and disease control rate (DCR) in the NDP group were 48.6% and 95.2%, significantly higher than in the DDP group at 35.1% and 89.2%, respectively (P<0.01). In NSCLC patients with initial treatment, squamous carcinoma and phase III, there were significant differences in ORR and DCR between the groups (P<0.05), while ORR was significant in patients with adenocarcinoma, GP/TP and in phase IIIa (P<0.05). There was also a significant difference in DCR in patients in phase IIIb (P<0.05). According to the statistical analysis of survival time of all patients and of those in clinical phase III, the NDP group survived significantly longer than the DDP group (P<0.01). The rates of decreased hemoglobin and increased creatinine, nausea and vomiting in the NDP group were evidently lower than in DDP group (P<0.05). Conclusion: NDP concomitant with other chemotherapy is effective for treating NSCLC, with higher clinical efficacy than DDP concomitant with chemotherapy, with advantages in prolonging survival time and reducing toxic and adverse responses.

Characterization of mitochondrial genomes of three new species: Leptocimbex praiaformis, L. clavicornis, and L. yanniae (Hymenoptera: Cimbicidae)

Yalan CHENG,Yuchen Yan,Mei-Cai WEI,Gengyun NIU 한국곤충학회 2021 Entomological Research Vol.51 No.6

Three new species of Leptocimbex, L. praiaformis, L. clavicornis, and L. yanniae, from China were described and illustrated. Nearly complete mitochondrial genomes were sequenced using next-generation sequencing. Compared to the ancestral organization, four tRNA genes were rearranged: trnM (+) and trnQ () are shuffled, while trnC ()andtrnY () are translocated from the trnW-trnC-trnY cluster to a location upstream of trnI. By mapping gene order onto phylogenies estimated from the mitochondrial genome, the evolutionary histories of gene rearrangement traits can be deciphered. All protein-coding genes are initiated by ATN codons and terminated with TAN, or T codons. By labeling the mutation on predicted secondary structures of rRNAs, the highly conserved motifs of rrnS (H769, H921, H939, H1506) also show phylogenetic signals. Phylogenetic relationships among the Symphyta were reconstructed using mitogenomes. Both maximum likelihood and Bayesian inference analyses highly support the monophyly of the genus Leptocimbex and the sister-group relationship between Leptocimbex and Labriocimbex + Trichiosoma.

Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells

Huang, Gang,Dang, Zhong-Feng,Dang, Ya-Mei,Cai, Wei,Li, Yuan,Chen, Yi-Rong,Xie, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Purpose: To study the expression of insulin-like growth factor binding proteins (IGFBPs) in paclitaxel-treated gastric cancer SGC-7901 cells, and to further investigate underlying mechanisms. Materials and Methods: Real time PCR and Western blot assays were applied to detect the mRNA and protein expression of IGFBP-2, -3 and -5 after paclitaxel (10 nM) treatment of SGC-7901 cells. In addition IGFBP-3 expression was silenced by RNA interference to determine effects. Cell viability was determined by MTT assay. Cell cycling and apoptosis were assessed by flow cytometry. Results: Compared to the control group, only IGFBP-3 expression was elevated significantly after paclitaxel (10 nM) treatment (p<0.05). Paclitaxel treatment caused cell cycle arrest and apoptosis via downregulating Bcl-2 expression. However, the effect could be abrogated by IGFBP-3 silencing. Conclusions: IGFBP-3 exhibits anti-apoptotic effects on paclitaxel-treated SGC-7901 cells via elevating Bcl-2 expression.

Mechanisms of Electroacupuncture Pretreatment in Alleviating Myocardial Ischemia Reperfusion Injury: Interactions between the Cerebellar Fastigial Nucleus and Lateral Hypothalamic Area

Yu Qing,Wu Li-bin,Zhang Fan,Wei Xiao-tong,Chen Pian-pian,Wang Shuai-ya,Cai Mei-yi,Shu Qi,Li Liao-yuan,Wu Zi-jian,Cai Rong-lin,Hu Ling 사단법인약침학회 2021 Journal of Acupuncture & Meridian Studies Vol.14 No.6

Background: Myocardial ischemia reperfusion injury (MIRI) is an important mechanism of post-myocardial infarction injury and a main cause of death in patients with ischemic heart disease. Electroacupuncture (EA) pretreatment is effective for the prevention and treatment of MIRI, but mechanisms mediating the effects of cardiovascular disease EA treatments remain unclear. Objectives: To determine whether the lateral hypothalamus (LHA) and the cerebellar fastigial nucleus (FN) are involved in the protective effects of EA stimulation on MIRI. Methods: EA pretreatment was performed for 7 days before the establishment of the MIRI model. ST-segment changes on electrocardiograms were recorded and the Curtis–Walker arrhythmia score was used to evaluate changes in reperfusion injury. Hematoxylin–eosin staining was applied to evaluate the pathological and morphological changes in myocardial tissue. c-fos expression in the LHA and FN was determined by immunofluorescence staining. Glutamic (Glu) and γ-Aminobutyric acid (GABA) levels were measured using a high-performance liquid chromatography-electrochemical method. Results: EA pretreatment reduced ST-segment elevation, arrhythmia scores, and morphological changes in MIRI myocardial cells in rats, and decreased the c-fos protein expression in LHA/FN nuclei. MIRI was associated with an imbalance between GABA and Glu levels, whereas EA pretreatment increased GABA levels and decreased Glu levels in the LHA/FN. Conclusion: FN and LHA are involved in the EA-mediated attenuation of MIRI. Pretreatment with EA plays a protective role in the myocardium by regulating Glu and GABA release in the LHA and FN.