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RISS 인기검색어
- 검색키워드
- 저자 : Md. Mashiar Rahman (검색결과 2 건)
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Role of RNase on Microbial Community Analysis in the Rice and Wheat Plants Soil by 16S rDNA-DGGE
Md. Mizanur Rahman,Abul Kalam Azad,Shamima Nasrin Sima,Md. Mashiar Rahman 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4
In this work, we reported the role of RNase on microbial community analysis from soil samples. The amounts of chemical componentswere measured from the adjoining soil of rice and wheat plants. The chemical properties of these soils were rich in surfacelevel as compared to other soil layers. Total DNA was extracted from freshly collected soils. To obtain pure DNA, half of the samplewas incubated with RNase and half was without RNase. A total of 16S rDNA fragments were amplified and analyzed by DenaturingGradient Gel Electrophoresis (DGGE). RNase-treated DNA followed by PCR resulted in a PCR product of good yield and integrity. DGGE bands and cluster analysis indicated a significant difference between the patterns obtained from RNase-treated and RNasenon-treated samples. The sequencing results of 16S rDNA fragments from DGGE profiles of soil samples indicated that the dominantbacteria are composed of different gene sequences. This is an interesting preliminary observation which indicates that the RNasetreatment prior to amplification and DGGE analysis modifies the pattern obtained. Therefore, it is suitable for community analysis ofagricultural plant soil samples as well as environmental samples
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Akhter, Shahina,Rahman, Md Mashiar,Lee, Hyun Seo,Kim, Hyeon-Jin,Hong, Seong-Tshool Springer ; Higher Education Press 2013 Protein & cell Vol.4 No.3
<P>Recent advances in hematopoietic stem cells (HSCs) expansion by growth factors including angiopoietin-like proteins (Angptls) have opened up the possibility to use HSCs in regenerative medicine. However, the unavailability of true in vitro HSCs expansion by these growth factors has limited the understanding of the cellular and molecular mechanism of HSCs expansion. Here, we report the functional role of mouse Angptls 1, 2, 3, 4, 6 and 7 and growth factors SCF, TPO, IGF-2 and FGF-1 on purified mouse bone-marrow (BM) Lineage(-)Sca-1(+)(Lin-Sca-1(+)) HSCs. The recombinant retroviral transduced-CHO-S cells that secrete Angptls in serum-free medium were used alone or in combination with growth factors (SCF, TPO, IGF-2 and FGF-1). None of the Angptls stimulated HSC proliferation, enhanced or inhibited HSCs colony formation, but they did support the survival of HSCs. By contrast, any of the six Angptls together with saturating levels of growth factors dramatically stimulated a 3- to 4.5-fold net expansion of HSCs compared to stimulation with a combination of those growth factors alone. These findings lead to an understanding of the basic function of Angptls on signaling pathways for the survival as well as expansion of HSCs in the bone marrow niche.</P>
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