Improving the physicochemical and antioxidative properties of fermented goat milk using carob molasses and some probiotic strains

Mahmoud Ibrahim El-Sayed,Esmat Aly,Amany Mohammed El-Deeb 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.3

Fermented goat milk samples, processed by adding 0, 2, and 4% carob molasses, and Bifidobacterium bifidum DSMZ or Lactobacillus helveticus CH5, were evaluated for their physicochemical, antioxidative, and sensory properties along with probiotic viability during storage. pH and viscosity gradually decreased over the storage period whereas their values increased with the increased amount of carob molasses. Bifidobacterium bifidum DSMZ or Lactobacillus helveticus CH5 kept higher viable numbers. Lactobacillus helveticus CH5 strain recorded higher viability. Increasing the added amount of carob molasses increased the total phenolic content, which in turn is reflected in the increase in antioxidant activity. Adding carob molasses participated in the partial masking of the goaty flavor and had no significant effect on the product’s acceptability, which was strongly influenced by the progress of cold storage. Therefore, fermented goat milk described by its enhanced physicochemical and antioxidative properties could be obtained by adding carob molasses and probiotics.

Preemptive analgesic efficacy of ultrasound-guided transversalis fascia plane block in children undergoing inguinal herniorrhaphy: a randomized, double-blind, controlled study

Abdelbaser Ibrahim,Mageed Nabil A.,El-Emam El-Sayed M.,ALseoudy Mahmoud M.,Elmorsy Mohamed M. 대한마취통증의학회 2021 Korean Journal of Anesthesiology Vol.74 No.4

Background: Surgical repair of congenital inguinal hernia results in significant postoperative discomfort and pain. The aim of the current study was to evaluate the pre-emptive analgesic efficacy of a transversalis fascia plane (TFP) block after pediatric inguinal herniorrhaphy.Methods: Forty-four patients aged 12 to 60 months who underwent unilateral inguinal herniorrhaphy were enrolled. Four patients were excluded, and the remaining were allocated to the control group and the TFP block group. In the TFP block group, 0.4 mL/kg bupivacaine 0.25% was instilled in the plane between the transversus abdominis and transversalis fascia, while in the control group 0.9% saline was used instead of bupivacaine. The collected data were the total dose of paracetamol consumed during the first 12 h postoperatively, the postoperative Face, Leg, Activity, Cry, Consolability (FLACC) pain score, time to first use of rescue analgesia, number of patients required additional postoperative analgesics, and parents' satisfaction.Results: The median paracetamol consumption was significantly lower in the TFP block group than in the control group, and FLACC pain scores were significantly lower for all study times in the TFP block group with higher parental satisfaction scores than those for the control group. The number of patients who required additional analgesics was significantly lower in the TFP block group than in the control group. Conclusions: The use of a TFP block decreases postoperative analgesic consumption and postoperative pain intensity after pediatric inguinal herniorrhaphy. Future studies with larger sample size are required to evaluate the actual complications rate of TFP block.

Disease Progression from Chronic Hepatitis C to Cirrhosis and Hepatocellular Carcinoma is Associated with Increasing DNA Promoter Methylation

Zekri, Abd El-Rahman Nabawy,Nassar, Auhood Abdel-Monem,El-Rouby, Mahmoud Nour El-Din,Shousha, Hend Ibrahim,Barakat, Ahmed Barakat,El-Desouky, Eman Desouky,Zayed, Naglaa Ali,Ahmed, Ola Sayed,Youssef, A Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Changes in DNA methylation patterns are believed to be early events in hepatocarcinogenesis. A better understanding of methylation states and how they correlate with disease progression will aid in finding potential strategies for early detection of HCC. The aim of our study was to analyze the methylation frequency of tumor suppressor genes, P14, P15, and P73, and a mismatch repair gene (O6MGMT) in HCV related chronic liver disease and HCC to identify candidate epigenetic biomarkers for HCC prediction. Materials and Methods: 516 Egyptian patients with HCV-related liver disease were recruited from Kasr Alaini multidisciplinary HCC clinic from April 2010 to January 2012. Subjects were divided into 4 different clinically defined groups - HCC group (n=208), liver cirrhosis group (n=108), chronic hepatitis C group (n=100), and control group (n=100) - to analyze the methylation status of the target genes in patient plasma using EpiTect Methyl qPCR Array technology. Methylation was considered to be hypermethylated if >10% and/or intermediately methylated if >60%. Results: In our series, a significant difference in the hypermethylation status of all studied genes was noted within the different stages of chronic liver disease and ultimately HCC. Hypermethylation of the P14 gene was detected in 100/208 (48.1%), 52/108 (48.1%), 16/100 (16%) and 8/100 (8%) among HCC, liver cirrhosis, chronic hepatitis and control groups, respectively, with a statistically significant difference between the studied groups (p-value 0.008). We also detected P15 hypermethylation in 92/208 (44.2%), 36/108 (33.3%), 20/100 (20%) and 4/100 (4%), respectively (p-value 0.006). In addition, hypermethylation of P73 was detected in 136/208 (65.4%), 72/108 (66.7%), 32/100 (32%) and 4/100 (4%) (p-value <0.001). Also, we detected O6MGMT hypermethylation in 84/208 (40.4%), 60/108 (55.3%), 20/100 (20%) and 4/100 (4%), respectively (p value <0.001. Conclusions: The epigenetic changes observed in this study indicate that HCC tumors exhibit specific DNA methylation signatures with potential clinical applications in diagnosis and prognosis. In addition, methylation frequency could be used to monitor whether a patient with chronic hepatitis C is likely to progress to liver cirrhosis or even HCC. We can conclude that methylation processes are not just early events in hepatocarcinogenesis but accumulate with progression to cancer.

Genetic parameters and principal components analysis of breeding value for birth and weaning weight in Egyptian buffalo

Salem, Mohamed Mahmoud Ibrahim,Amin, Amin Mohamed Said,Ashour, Ayman Fouad,Ibrahim, Mohamed Mohamed El-said,Abo-Ismail, Mohammed Kotb Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.1

Objective: The objectives of the current study were to study the main environmental factors affecting birth weight (BW) and weaning weight (WW), estimate variance components, genetic parameters and genetic trend and to evaluate the variability and relationships among breeding value of BW and WW using principal components analysis (PCA). Methods: A total of 16,370 records were collected from 8,271 buffalo calves. Genetic parameters and breeding values were estimated using a bivariate animal model which includes direct, maternal and permanent maternal effects. These estimates were standardized and used in PCA. Results: The direct heritability estimates were 0.06 and 0.41 for BW and WW, respectively whereas direct maternal heritability values were 0.03 and 0.14, respectively. Proportions of variance due to permanent environmental effects of dam were 0.455 and 0.280 for BW and WW respectively. The genetic correlation between BW and WWs was weak approaching zero, but the maternal correlation was 0.26. The first two principal components (PC1 and PC2) were estimated utilizing the standardized breeding values according to Kaiser method. The total variance explained by the first two PCs was 71.17% in which 45.91% and 25.25% were explained by PC1 and PC2, respectively. The direct breeding values of BW were related to PC2 but those of WW and maternal breeding values of BW and WWs were associated with PC1. Conclusion: The results of genetic parameters and PCA indicate that BW and WWs were not genetically correlated and improving growth traits of Egyptian buffaloes could be achieved using WW without any adverse effect by BW.

Synergistic impact of multiwalled carbon nanotubes on the properties of Ni-Mo thin-film via electrodeposition technique

Mona Hasan Gomaa,Zeinab Abdel Hamid,Magdy Ahmed Mahmoud Ibrahim,Rania Abd El Sttar,El-Said Helmy El- Mosallamy 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.5

Carbon nanotubes (CNTs) are the hardest and strongest materials due to their perfect mechanical properties and excellent chemical, electrical, and thermal characteristics. Therefore, CNTs are attractive candidates for the development of innovative multifunctional nanocomposites. The goal of the study was to synthesize and characterize NiMoCNT nanocomposite coatings onto steel substrates by electrodeposition technique to enhance the properties of the NiMo layer coating. The electrodeposition was carried out galvanostatically, and the percentage of MWCNT (wt%) in the composites was investigated under various working circumstances, including current density, pH, temperature, and CNTs concentration in the electroplating bath. Different techniques, such as X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and energy dispersive X-ray analysis (EDS), were used for the composite characterization. Moreover, the corrosion resistance and the nanocomposites’ mechanical characteristics were investigated. The results of the NiMo alloy show that the enhancement in current density decreases the Mo content from 41.8 wt% (at 3.5×10−2 Acm−2) to 31.06 wt% (at 6.5×10−2 Acm−2). On the other hand, the results proved that as the concentration of CNTs in the bath increases, the wt% of CNTs co-deposited in the NiMo matrix enhances, peaking at 22.36 wt% at 0.03 gL−1. Furthermore, the findings show that the Mo content of the coating is reduced when CNTs are present. In comparison to a NiMo coating without CNTs, the composite incorporating CNTs exhibits better corrosion resistance. In addition, the mechanical properties show that the microhardness of NiMoCNT composite-coated steel is better than that of NiMo, and the highest microhardness of NiMoCNT composite coated steel was 4.69 GPa, while pure NiMo coated steel had a microhardness of 2.37 GPa.