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RISS 인기검색어
- 검색키워드
- 저자 : Mahdi Ahouran (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Corms as a Source of Explants for the Successful Clonal Propagation of $Crocus$ $cancellatus$
Ahouran, Mahdi,Hosseini, Ramin,Zarghami, Reza 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1
The genus $Crocus$ comprises plants with a potential to be developed as a new ornamental crop but to date, there are not many reports on $in$ $vitro$ propagation of many members of this genus. The present study involves $in$ $vitro$ propagation of $Crocus$ $cancellatus$ with ornamental and horticultural value. Two different types of corm explants (apical and basal halves of corms) were cultivated onto Murashige and Skoog's (MS) medium supplemented with different levels of ${\alpha}$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). One to five cormlets emerged from every responding explant through direct organogenesis. Apical halves of corms were more highly responsive than basal halves and produced a maximum multiplication rate with $3.45{\pm}0.06$ cormlets per explant in $95.33{\pm}2.33%$ of the explants in MS medium supplemented with 3% sucrose and $2mg\;L^{-1}$ NAA and $1mg\;L^{-1}$ BAP. The effect of cold storage temperature on in vitro cormlets sprouting was studied. Cormlets stored at $4^{\circ}C$ for 8 weeks had more statistically significant positive effects on cormlets sprouting from the controls. $In$ $vitro$ rooting of cormlets was induced on MS medium without plant hormones.
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Corms as a Source of Explants for the Successful Clonal Propagation of Crocus cancellatus
Mahdi Ahouran,Ramin Hosseini,Reza Zarghami 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1
The genus Crocus comprises plants with a potential to be developed as a new ornamental crop but to date, there are not many reports on in vitro propagation of many members of this genus. The present study involves in vitro propagation of Crocus cancella-tus with ornamental and horticultural value. Two different types of corm explants (apical and basal halves of corms) were cultivated onto Murashige and Skoog’s (MS) medium supplemented with different levels of α-naphthalene acetic acid (NAA) and 6-benzy-laminopurine (BAP). One to five cormlets emerged from every responding explant through direct organogenesis. Apical halves of corms were more highly responsive than basal halves and produced a maximum multiplication rate with 3.45 ± 0.06 cormlets per explant in 95.33 ± 2.33% of the explants in MS medium supplemented with 3% sucrose and 2 mg L -1 NAA and 1 mg L -1 BAP. The effect of cold storage temperature on in vitro cormlets sprouting was studied. Cormlets stored at 4°C for 8 weeks had more statistical-ly significant positive effects on cormlets sprouting from the controls. In vitro rooting of cormlets was induced on MS medium with-out plant hormones.
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