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Surface electrochemistry for the construction of highly sensitiveimmunoassay system
Fumio Mizutani,Hiroaki Matsuura 한국물리학회 2005 Current Applied Physics Vol.5 No.2
The determination of acetycholinesterase (AChE) was carried out based on the chemisorption/reductive desorption of thiocho-line on a silver electrode surface. Thiocholine, which was produced through the hydrolysis of acetylthiocholine with AChE, chemi-sorbed on the silver surface during an incubation time; then the silver electrode was transferred into an alkaline solution and theadsorbent was electrochemically desorbed. The increase in the enzymatic activity resulted in the increase of the number of the thio-choline molecules chemisorbed and, accordingly, that of the cathodic current response for the electrochemical desorption. The thiolmolecules were accumulated on the electrode surface through the chemisorption, which resulted in an enhanced sensitivity for meas-uring the enzyme activity. The detection limit for AChE was 0.01 Ul. 1, i.e., a hundred times lower than that obtained by a con-ventional amperometric sensor. This method was successfully applied to the highly sensitive measurement of a peptide hormone,B-type natriuretic peptide (BNP), with the use of the enzyme-labeled anti-BNP antibody.. 2004 Elsevier B.V. All rights reserved.PACS:82.80.Fk
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KUROIWA Yuichiro,MATSUURA Hiroaki,ABE Atsushi,KATO Mugio,ARIJI Yoshiko,KURITA Kenichi 대한구강악안면외과학회 2008 대한구강악안면외과학회지 Vol.34 No.3
Mandibular chronic osteomyelitis with diffuse osteosclerosis is recognized as an intractable infectious disease. We emplyed decortication and intraarterial infusion of antibiotics in 6 cases. Decortication on the affected mandible was performed with retrocatherization to the superficial temporal artery of affected side under general anesthesia. Antibiotics, IPM/CS or FOMX was used through the artery for 4-11 days. In addition, we administered FOMX, PIPC intravenously for 8-17 days. CT and MRI were taken postoperatively. The postoperative follow-up period ranged from 1 year and 6 months to 2 years and 5 months. Postoperative MR showed that bone marrow signal was recovered to approximately normal in 4 cases. High signal area of bone marrow and osteosclerosis image remained in 2 cases, but showed improvement. The results were satisfactory without recurrence in all of 6 cases.
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