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Yu Zheyuan,Xie Huicong,Liu Yijiao,Li Kai,He Zhuqing 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
The crickets of genus Sclerogryllus Gorochov, 1985 belongs to subfamily Sclerogryllinae of family Gryllidae. In this study, we report the first complete mitogenome sequences of the genus Sclerogryllus, and analyze the features of mitogenomes of S. punctatus. The mitogenome of S. punctatus was 15,438 bp and consisted of 37 genes, coding for 13 proteins, 2 ribosomal RNA (rRNA) and 22 transfer RNA (tRNA), and a control region. S. punctatus shares the arrangement of trnE-trnS-trnN with most mitogenomes of Grylloidea. Besides, the tRNAs possess the typical cloverleaf secondary structure except for the trnS1 (AGN) gene. The phylogenetic analysis using 13 proteincoding genes and 2 rRNA represents that genus Sclerogryllus is included in subfamily Gryllinae. Our results uncover the phylogenetic position of genus Sclerogryllus by mitogenome data within the family Gryllidae.
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Yu Zheyuan,Xie Huicong,Liu Yijiao,Li Kai,He Zhuqing 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
The crickets of genus Sclerogryllus Gorochov, 1985 belongs to subfamily Sclerogryllinae of family Gryllidae. In this study, we report the first complete mitogenome sequences of the genus Sclerogryllus, and analyze the features of mitogenomes of S. punctatus. The mitogenome of S. punctatus was 15,438 bp and consisted of 37 genes, coding for 13 proteins, 2 ribosomal RNA (rRNA) and 22 transfer RNA (tRNA), and a control region. S. punctatus shares the arrangement of trnE-trnS-trnN with most mitogenomes of Grylloidea. Besides, the tRNAs possess the typical cloverleaf secondary structure except for the trnS1 (AGN) gene. The phylogenetic analysis using 13 proteincoding genes and 2 rRNA represents that genus Sclerogryllus is included in subfamily Gryllinae. Our results uncover the phylogenetic position of genus Sclerogryllus by mitogenome data within the family Gryllidae.
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Mingqi Tang,Weiping Li,Huicong Liu,Liqun Zhu 한국물리학회 2012 Current Applied Physics Vol.12 No.5
Black and gray microarc oxidation (MAO) coatings were prepared in a phosphate electrolyte with and without K2TiF6 on 2A70 aluminum alloy, respectively. Voltageetime curves were recorded during the MAO process. The effects of K2TiF6 on the morphology, composition, abrasive resistance and corrosion resistance of MAO coatings were investigated. The results showed that the MAO coating produced in the electrolyte with K2TiF6 was thicker, and more uniform than that produced in the electrolyte without K2TiF6. Ti was detected in the surface of the MAO coating formed in the electrolyte with K2TiF6. The results of abrasive resistance and corrosion resistance showed that the MAO coating formed in the electrolyte with K2TiF6 exhibited better abrasive resistance and corrosion resistance.
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Haining Chen,Liqun Zhu,Weiping Li,Huicong Liu 한국물리학회 2012 Current Applied Physics Vol.12 No.1
In this paper, we reported an investigation on a new photoelectrode of quantum dots-sensitized solar cell (QDSC) combining indiumetineoxide (ITO) mesoporous film and CdS quantum dots (QDs). The ITO mesoporous film was prepared by doctor-blade technique and CdS QDs attached on ITO mesoporous film were synthesized by successive ionic layer adsorption and reaction method. X-ray diffraction, field emission scanning electron microscopy, transmission electron microscope, X-ray spectroscopy and UV evis spectroscopy were used to characterize the samples. The results indicated that the ITO mesoporous film was uniform, crack-free and highly porous. And absorbance in visible region was enhanced after the deposition of CdS QDs on ITO mesoporous film. The photoelectrochemical property of the CdS QDssensitized ITO mesoporous film photoelectrode was investigated by forming a photoelectrochemical cell. Photocurrentevoltage measurement showed that the photoelectrode was efficient in the cell as working electrode.
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Epithelial CST1 Promotes Airway Eosinophilic Inflammation in Asthma via the AKT Signaling Pathway
Du Lijuan,Xu Changyi,Tang Kun,Shi Jia,Tang Lu,Lisha Xiao,Lei Chengcheng,Liu Huicong,Liang Yuxia,Guo Yubiao 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.3
Purpose: Epithelial cystatin SN (CST1), a type 2 cysteine protease inhibitor, was significantly upregulated in asthma. In this study, we aimed to investigate the potential role and mechanism of CST1 in eosinophilic inflammation in asthma. Methods: Bioinformatics analysis on Gene Expression Omnibus datasets were used to explore the expression of CST1 in asthma. Sputum samples were collected from 76 asthmatics and 22 control subjects. CST1 mRNA and protein expression in the induced sputum were measured by real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blotting. The possible function of CST1 was explored in ovalbumin (OVA)-induced eosinophilic asthma. Transcriptome sequencing (RNA-seq) was used to predict the possible regulated mechanism of CST1 in bronchial epithelial cells. Overexpression or knockdown of CST1 was further used to verify potential mechanisms in bronchial epithelial cells. Results: CST1 expression was significantly increased in the epithelial cells and induced sputum of asthma. Increased CST1 was significantly associated with eosinophilic indicators and T helper cytokines. CST1 aggravated airway eosinophilic inflammation in the OVA-induced asthma model. In addition, overexpression of CST1 significantly enhanced the phosphorylation of AKT and the expression of serpin peptidase inhibitor, clade B, member 2 (SERPINB2), while knockdown using anti-CST1 siRNA reversed the trend. Furthermore, AKT had a positive effect on SERPINB2 expression. Conclusions: Increased sputum CST1 may play a key role in the pathogenesis of asthma through involvement in eosinophilic and type 2 inflammation through activation of the AKT signaling pathway, further promoting SERPINB2 expression. Therefore, targeting CST1 might be of therapeutic value in treating asthma with severe and eosinophilic phenotypes.
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