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        Transcriptome explores changes in gene expression profile contributed by Camellia sinensis (L.) in Apis cerana cerana and Apis mellifera ligustica

        Zhao Dongxu,Chen Daoyin,Su Xiaoling,Zhang Xiaojun,Liu Yanjie 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

        Camellia sinensis (L.) is widely cultivated in China and other countries as an economically important tree and supplies unique nutrients to ensure the development of Apis cerana cerana and Apis mellifera ligustica colonies in early spring in southern China. A. c. cerana was domesticated in China many years ago and exhibits a smaller body size, smaller colony size and some distinct physiology and behavior compared to A. m. ligustica. Here, to explore the changes in gene expression induced by C. sinensis in A. c. cerana and A.m. ligustica, we performed transcriptomic analysis of the 1 st -instar, 5 th -instar, 1 st -adult and 7 th -adult stages of the two species fed the nectar and pollen of C. sinensis. A total of 489, 1190, 991 and 243 differentially expressed genes (DEGs) were identified in the four developmental stages of A. m. ligustica, and 995, 2785, 1513 and 213 DEGs were found in the cor responding stages of A. c. cerana. The changes in the gene expression profile were more significant in the 5 th -instar and 1 st -adult. Furthermore, the upregulated genes in the 5 th -instar and 1 st -adult were mainly enriched in pathways involved in growth- and development-related pathway: Hippo signaling pathway. In addition to the upregulated DEGs, growth- and development-related pathways, including the Wnt signaling pathway, Hedgehog signaling pathway-fly, and FoxO signaling pathway, were enriched in A. c. cerana. Finally, we hypothesized that C. sinensis pollen is beneficial to the growth and development of honeybees and give rise to diverse corresponding regulation to A. c. cerana than to A. m. ligustica.

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        Effect of moisture content on dense-phase conveying of pulverized coal at high pressure

        Xu Pan,Chen Xiaoping,Liang Cai,Xu Guiling,Liu Daoyin,Zhao Changsui 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.10

        In dense-phase pneumatic conveying, the solid moisture content can significantly influence the conveying process, but there are very few studies in the open literature. In this study, the conveying experiments of two pulverized coals with various moisture contents were carried out at a 4MPa high pressure and dense-phase pneumatic conveying facility. Results show that the whole conveying system can be stably and controllably operated under the condition that moisture content below ~8%. With the increase of moisture content up to ~8%, the mass flow rate of 280 μm pulverized coal increases at first and then decreases, while that of 55 μm pulverized coal decreases continuously. The method of solid friction factor correlation is used to investigate pressure drop of the horizontal pipe, and non-dimensional parameters--Fr number, moisture content M and solid loading ratio μ--are investigated. The pressure drop predictions by this correlation are in good agreement with the experimental data. The solid friction factor correlations indicate that the fine coal is more sensitive to M, and μ plays a more important role for the coarse coal.

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