Preliminary Imaging Analysis for Enhanced Intestinal Uptake of Non-soluble Polystyrene Microspheres in the Presence of Oleic Acid using Rat Intestine

Tran, Huyen Thi Thanh,Tran, Phuong Ha Lien,Tran, Thao Truong-Dinh,Lee, Kyung-Ho,Lee, Beom-Jin The Korean Society of Pharmaceutical Sciences and 2009 Journal of Pharmaceutical Investigation Vol.39 No.3

In vitro intestinal uptake of non-soluble polystyrene microspheres (NPMS) was visualized with and without oleic acid using a fluorescence microscopy. Fluorescent polystyrene latex microspheres with 1${\mu}$m larger size were used as models for nonspecifically absorbed nonbiodegradable particulates. The NPMS could not penetrate the enterocytes but a few NPMS could be penetrated via Peyer's patches. When the oleic acid was mixed with NPMS, the transporting efficiency of NPMS through enterocytes as well as Peyer's patches was significantly enhanced. The modification of the intestinal membrane permeability and surface feature of the NPMS in the presence of oleic acid might be a clue to the transport of NSPM although the detailed mechanism is still under investigation.

Roles of MgO Release from Polyethylene Glycol 6000-based Solid Dispersions on Microenvironmental pH, Enhanced Dissolution and Reduced Gastrointestinal Damage of Telmisartan

Phuong Ha Lien Tran,이범진,Thao Truong-Dinh Tran,Seung Aeon Lee,Vo Hong Nho,Sang-Cheol Chi 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.5

The roles of magnesium oxide (MgO) release from solid dispersions (SDs) in simulated gastric fluid (SGF), simulated intestinal fluid (SIF) and water were investigated to elucidate the enhanced dissolution and reduced intestinal damages of telmisartan as a model drug. The polyethylene glycol 6000 (PEG 6000) was used to prepare the SDs. Three SDs were prepared: SD1(PEG, MgO, TEL), SD2 (PEG 6000, TEL), SD3 (MgO, TEL). The physical mixture (PM) consisting of SD2 and MgO was also prepared. A binary SD without MgO (SD2) was also prepared for comparison in microenvironmental pH (pH_M) modulation. The faster MgO released, the less control of pH_M and the less enhanced dissolution of TEL were in consequences. SD3 increased dissolution in SIF and water (about 67%). Interestingly, ternary SD1 showed almost complete dissolution in all three media but dissolution of PM was the lowest due to the fast release of MgO and poor modulation of pHM. MgO did not change the drug crystallinity but did have a strong molecular interaction with the drug. Additionally, the SD3-bearing tablet quickly increased pHM but then gradually decreased due to faster release of MgO while the SD1-bearing tablet gradually increased pH_M at all fractional dimensions of the tablet by the MgO slowly released. The pH_M of PM-bearing tablets was not varied as a function of time. Thus, the MgObearing SD1 also minimized gastrointestinal tissue damage caused by the model drug.

Promising Iron Oxide-based Magnetic Nanoparticles in Biomedical Engineering

Phuong Ha-Lien Tran,Thao Truong-Dinh Tran,Toi Van Vo,이범진 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12

For the past few decades biomedical engineering has imprinted its significant impact on the map of science through its wide applications on many other fields. An important example obviously proving this fact is the versatile application of magnetic nanoparticles in theranostics. Due to preferable properties such as biocompatibility, non-toxicity compared to other metal derivations, iron oxide-based magnetic nanoparticles was chosen to be addressed in this review. Aim of this review is to give the readers a whole working window of these magnetic nanoparticles in the current context of science. Thus, preparation of magnetic iron oxide nanoparticles with the so-far techniques, methods of characterizing the nanoparticles as well as their most recent biomedical applications will be stated.

Preliminary Imaging Analysis for Enhanced Intestinal Uptake of Non-soluble Polystyrene Microspheres in the Presence of Oleic Acid using Rat Intestine

Huyen Thi Thanh Tran,Phuong Ha Lien Tran,Thao Truong Dinh Tran,Kyung Ho Lee,Beom Jin Lee 韓國藥劑學會 2009 Journal of Pharmaceutical Investigation Vol.39 No.3

Fattigation-platform theranostic nanoparticles for cancer therapy

Tran, Thao Truong-Dinh,Tran, Phuong Ha-Lien,Yoon, Tae-Jong,Lee, Beom-Jin Elsevier 2017 Materials science & engineering. C, Materials for Vol.75 No.-

<P>A new conceptual nanoparticle consisting of a silica-coated iron oxide magnetic core and a fattigation-based biocompatible shell with oleic acid and hydrophilic protein (gelatin). The prepared particle can be a useful theranostics platform material for diagnostic imaging and as a drug delivery system. Oleic acid and gelatin were conjugated on the silica-coated magnetic nanoparticle surface to provide three primary functionalities: 1) enhancing biocompatibility and solubility in aqueous solution and providing the ability to incorporate hydrophobic chemical drugs into the shell for delivery, 2) improving treatment-response magnetic monitoring as a diagnostic agent with low nanotoxicity, and 3) increasing anticancer efficacy owing to the controlled release of the incorporated drug in cells and in an animal model. We prepared magnetic-silica nanoparticles with super paramagnetic properties, which are utilized as a T-2-weighted magnetic resonance imaging agent. After formation of an oleic acid-gelatin shell, the prepared materials exhibited high loading capacity for a hydrophobic anticancer drug (paclitaxel). Our particle platform system exhibited higher therapeutic efficacy and lower toxicological effects in vitro and in an in vivo cancer model than a clinically available chemo-drug (Taxol (R)). Our findings strongly suggest that this nanoparticle system can serve as a platform for cancer therapy by the incorporation of chemical drugs. (C) 2017 Elsevier B.V. All rights reserved.</P>

Expression analyses of soybean genes encoding methionine-Rsulfoxide reductase under various conditions suggest a possible role in the adaptation to stress

Ha Duc Chu,Kim-Lien Nguyen,Yasuko Watanabe,Dung Tien Le,Lam-Son Phan Tran 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.5

Under stress, plant cellular proteins can be oxidized at multiple amino acid residues causing protein dysfunction that may lead to reduced viability of plants. One of the oxidized amino acids, methionine sulfoxide (MetO), was frequently found in stressed plants. In soybeans, there are five methionine-R-sulfoxide reductases (GmMSRBs) responsible for the reduction of Met-R-O, one of the two MetO isomers. To identify GmMSRBs that may be involved in repairing Met-R-O under different stress conditions, we determined transcript levels of GmMSRBs in various tissues subjected to dehydration/drought, high salinity, and abscisic acid (ABA) during different developmental stages. Under normal conditions, expression levels were the highest in leaves, followed by roots, and lowest in seeds and seed pods. Among the GmMSRBs, transcripts of GmMSRB1 in the leaves were the highest; at the same time, GmMSRB5 was shown to be expressed at the lowest levels. Expression of GmMSRBs were then determined under stress-inducing conditions. In seedling shoots, GmMSRB2 and GmMSRB5 were expressed in response to drought conditions. In vegetative V6 trifolia, only GmMSRB3 was induced under drought. In reproductive R2 trifolia, the expression of GmMSRB2 and GmMSRB5 were induced by drought. However, expressions of all five GmMSRBs in the roots were not affected by the any stressinducing conditions. Under salt stress, GmMSRB1 was downregulated in seedling shoots and GmMSRB5 was up-regulated in seedling roots. Treatment with ABA did not affect the transcript levels of any GmMSRBs in seedling shoots. However, this treatment up-regulatesGmMSRB2 in seedling roots. Our data suggested that with the exception of GmMSRB4, all the remaining four GmMSRBs play a role in soybean responses to multiple environmental stresses and that genes encoding cytosolic and plastidic GmMSRBs respond differently under stress.

Biodistribution and <i>in vivo</i> performance of fattigation-platform theranostic nanoparticles

Tran, Thao Truong-Dinh,Tran, Phuong Ha-Lien,Amin, Hardik H.,Lee, Beom-Jin Elsevier 2017 Materials science & engineering. C, Materials for Vol.79 No.-

<P><B>Abstract</B></P> <P>This study was aimed at characterizing superparamagnetic nanoparticles surface-functionalized with gelatin-oleic acid (GOAS-MNPs) and loaded with paclitaxel by assessing the pharmacokinetics and biodistribution of paclitaxel in tissues and the <I>in vivo</I> efficacy of antitumor activity after the administration of the drug. Initially, instrumental analysis was performed to examine the particle size distribution, surface charge, and morphology of the paclitaxel-loaded GOAS-MNPs. Furthermore, we evaluated their magnetic properties and performed T2-weighted magnetic resonance imaging (MRI) on cells. We intravenously administered Taxol® and paclitaxel-loaded GOAS-MNPs and compared the pharmacokinetics, biodistribution, and antitumor efficacies of the two formulations. Determination of the pharmacokinetics and the biodistribution of paclitaxel-loaded NPs showed that this formulation increased the systemic circulation time of paclitaxel and regulated its transport to tissues. The <I>in vivo</I> antitumor efficacy of the paclitaxel-loaded NPs was better than that of Taxol® at the same dose. Furthermore, the paclitaxel-loaded GOAS-MNPs were found to be effective as contrast agents for enhanced MRI in cancer cells. Thus, GOAS-MNPs could be an effective diagnostic system for cancer and for the delivery of paclitaxel with better therapeutic effects and a significant reduction in toxicity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Improving therapeutic efficacy and lower toxicity </LI> <LI> A significant inhibition of tumor growth </LI> <LI> Could be used as MRI contrast agents </LI> </UL> </P>

Impact of Administrative Post and Gender on Lecturers' Research Motivation in Vietnam

TRAN, Thi Kim Nhung,NGUYEN, Thanh Do,PHAM, Thanh Van,NGUYEN, Thi Lien Huong,GIANG, Minh Duc,HA, Ngoc Thang Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.3

This study examines the simultaneous impact of gender and administrative post on the research motivation of university lecturers. There are 475 selected questionnaires that are qualified for data processing and the collected data are analyzed by two-way analysis of variance (ANOVA). The results show the relationship between administration involvement and scientific research of lecturers. In general, lecturers with administrative post have higher research motivation than lecturers without administrative post. More specifically, the level of administrative post has positive correlation with research motivation of female lecturers, but has negative correlation of male lecturers. It means that a female lecturer with higher administrative post also has more motivation to conduct research. Conversely, the higher administrative post a male lecturer has, the less research motivation he is. The cause of this difference is the pressure of earning money on Asian men, including Vietnamese men, who are considered to be main breadwinners of their family. When having an administration post, the more chances male lecturers have to earn money, the less time they can spend for scientific research. This suggests that the solution for managers is to perform annual assessment, fulfill promotion process, build up motivation and reward system, and establish recruitment and promotion policies.

Expression analyses of soybean genes encoding methionine-R-sulfoxide reductase under various conditions suggest a possible role in the adaptation to stress

Chu, Ha Duc,Nguyen, Kim-Lien,Watanabe, Yasuko,Le, Dung Tien,Tran, Lam-Son Phan The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.5

Under stress, plant cellular proteins can be oxidized at multiple amino acid residues causing protein dysfunction that may lead to reduced viability of plants. One of the oxidized amino acids, methionine sulfoxide (MetO), was frequently found in stressed plants. In soybeans, there are five methionine-R-sulfoxide reductases (GmMSRBs) responsible for the reduction of Met-R-O, one of the two MetO isomers. To identify GmMSRBs that may be involved in repairing Met-R-O under different stress conditions, we determined transcript levels of GmMSRBs in various tissues subjected to dehydration/drought, high salinity, and abscisic acid (ABA) during different developmental stages. Under normal conditions, expression levels were the highest in leaves, followed by roots, and lowest in seeds and seed pods. Among the GmMSRBs, transcripts of GmMSRB1 in the leaves were the highest; at the same time, GmMSRB5 was shown to be expressed at the lowest levels. Expression of GmMSRBs were then determined under stress-inducing conditions. In seedling shoots, GmMSRB2 and GmMSRB5 were expressed in response to drought conditions. In vegetative V6 trifolia, only GmMSRB3 was induced under drought. In reproductive R2 trifolia, the expression of GmMSRB2 and GmMSRB5 were induced by drought. However, expressions of all five GmMSRBs in the roots were not affected by the any stressinducing conditions. Under salt stress, GmMSRB1 was down-regulated in seedling shoots and GmMSRB5 was up-regulated in seedling roots. Treatment with ABA did not affect the transcript levels of any GmMSRBs in seedling shoots. However, this treatment up-regulates GmMSRB2 in seedling roots. Our data suggested that with the exception of GmMSRB4, all the remaining four GmMSRBs play a role in soybean responses to multiple environmental stresses and that genes encoding cytosolic and plastidic GmMSRBs respond differently under stress.

Customer Satisfaction with Less than Container Load Cargo Services in HoChiMinh City, Vietnam

GIAO, Ha Nam Khanh,THY, Nguyen Thi Anh,VUONG, Bui Nhat,TU, Tran Ngoc,VINH, Pham Quang,LIEN, Le Thi Phuong Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8

This research has four specific objectives: (1) identifying factors that affect customer satisfaction with less than container load (LCL) cargo services of logistics companies in HoChiMinh City (HCMC), (2) measuring the level of impact of the factors, (3) testing the difference in satisfaction among groups of customers with different characteristics in terms of type of business and time of using LCL cargo services, and (4) proposing some management implications to improve the quality of LCL cargo services. Researchers interviewed 210 customers who enjoyed the LCL cargo service in HCMC for at least the last six months, using the convenient sampling method. SPSS 20 was used to analyze the reliability of the scale through the Cronbach's alpha coefficient, then exploratory factor analysis and multiple linear regression analysis were used. The results identified the six factors that influence positively customer satisfaction of LCL cargo services of logistics companies in HCMC, by decreasing importance: service process, image, resource, price, management, and outcomes. The results show that there is no difference in customer satisfaction with LCL cargo services by types of business as well as time of using services. The research suggests some implications for the management of logistics companies in HCMC to enhance customer satisfaction.