催眠鎭靜劑의 生體的 脂肪蓄積誘發에 關한 硏究

高光三,車鍾希,朴順花 中央醫學社 1974 中央醫學 Vol.27 No.5

Administration of three hypnotic sedatives to rats fed a standard laborarory diet and five different diets affected the levels of triglyceride, phospholipid and cholesterol in the liver and serum, and the levels of lipase in the serum and pancrea. Phenobarbital and amobarbital produced the elevated levels of hepatic lipids and serum triglyceride, but not affected the levels of serum phospholid and cholesterol. Chlorpromazine did not affected the levels of serum and hepatic lipids. Choline,. Fat and protein contents in these diets were factors influencing the triglyceride accumulation in the liver as a result of phenobarbital and amobarbital. However, these dietary factors did not to regulate the manner in which phenobarbital and amobarbital elevated phospholipid and total cholesterol levels in the liver. The level of serum lipase was elevated by administration of amobarbital, and the level of pancreatic lipase was decreased by administration of chloropromazine.

韓國人의 赤血球 G-6-PD에 關한 硏究

高光三,金昌世 최신의학사 1973 最新醫學 Vol.16 No.6

1. On the studies of hemolyzing solution we were obtained such optimum concentration of enzyme G-6-PD activity as followings: 2.7mM of EDTA, 5mM beta-mecrcaptoethanol, and 10 pM NADP. 2. Many different experimental conditions with relation to the measurement of enzyme activity were studied and obtained as following results: a) The production rate of NADPH was constant during first 30 minutes after adding G-6-P into the reaction mixture. b) Enzyme concentration, 1 to 6 volumes of usual concentration, obeyed by Beer's law. c) Influence of temperature was so significant that we used to a temperature of 25°C throughout the experimeatal procedures. d) The reaction mixture except G-6-P was stable on 8 days or more in deep freezer (-45°C) and about 5 days in refrigerator (5°C). e) The samples to be examined were stable on 3 hours at room temperature in case of intact whole blood and on 1 hour or less in case of hemolysates. 3. Erythrocyte G-6-PD values in Korean was such as follows: The distribution of 294 males was 7.82±4.77, and 286 females 7.24±4.23 IU per gm of Hb. Age distributions of both groups, male and female, were 1 year or less to 75 years old which contained 5-10 healthy individuals in each age groups. 4. There was a groups strongly suggested such as G-6-PD Hektoen those enzymic value is shown about three times of mean value on the distribution curve.

遺傳工學的 利用을 위한 人體細胞培養에 대한 硏究(Ⅰ) : 人體包皮細胞培養에 影響을 주는 因子에 대하여 Human Foreskin CellCulture

高光三,金盛植,車鍾希,李根培,金成原 조선의대 1983 The Medical Journal of Chosun University Vol.8 No.1

쥐췌장 베타세포 소배양법의 생리적 검정

고광삼,김성식,김양균,이근배 朝鮮大學校 附設 醫學硏究所 1984 The Medical Journal of Chosun University Vol.9 No.1

쥐 췌장 베타세포의 소 배양법에 대한 생리적 반응을 알아보기 위하여 cell density를 측정하는 방법으로 DNA 농도를 측정하였고, cell density가 intracellular와 extracellular immunoreactive insulin (IRI) 농도에 미치는 영향을 검토하였으며, glucose, arginine, isoleucine 및 leucine이 insulin 분비에 미치는 영향을 알아보았다. 그 결과 DNA 농도는 배양후 2일째에 가장 높았으며, intracellular IRI은 배양후 6일째까지 점차적으로 증가되었고, extracellular IRI은 배양시작후 8일째까지 증가되었으나 그후 감소되었다. Cell density는 intracellular IRI와 extracellular IRI 농도에 영향을 주지 않았으며, glucose는 0~300㎎/100㎖ 농도에서 점차적으로 IRI 분비를 증가시켰으나 300~500㎎/100㎖ 농도에서는 변화가 없었다. Leucine은 IRI 분비를 3배증가시켰으며, arginine과 isoleucine은 2배 증가시켰다. 이로 미루어 보아 본 연구에 이용한 쥐 췌장 베타세포와 소 배양법으로 배양한 쥐 췌장 베타세포는 적절한 생리적 반응을 나타내고 있음을 알 수 있었다. Physiological responses of monolayered mouse beta cell vary according to culture conditons. These cells maintained normal morphological characteristics when cultured in a microculture system. Following determination of DNA concentration as a means of measuring cell density, the effect of cell density on intracellular and extracellular immunoreactive insulin (IRI) conccntration was determmed. Then the effect of glucose, arginine, isoleucine and lencine on insuline secretions was evaluated. When 2 davs old cultures with cell densities between 10^(3) and 10^(6) were compared. the increase of DNA concentration was highest in the 2 days old cultures and no further change by the 14th days. The intracellular IRI through the increase of cell population increased progressively up to culture day 6, and then slowly decreased. Extracellular IRI increased until day 8 and then decreased thereafter regardless cell density. The production of either intracellular or extracellular IRI was therefore independent from the density of cultured cells, Exposure to glucose in the range of 0 to 300㎎/100㎖ resulted in a progressive increase of IRI secretions, but from 300 to 500㎎/100㎖ there was no change. Exposure to leucine resulted in a three-fold increase of IRI secretions, whereas arginine and isoleucine caused these microcultured mouse beta cells to double their IRI secretions. This invetigation, therefore, not only demonstrated that cultured cells in the microculture system were morphologically normal, but also the system was suitable for studies of physiological responses to various culture conditions of murine pancreatic beta cells.

당뇨병을 유발하는 Virus에 대한 단일항체 생산세포의 C-type Virus와 Reverse Transcriptase에 관한 연구

배광묵,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

The correlation between localization of C-typs virus particles, reverse transcriptase activity and monoclonal antibody production by murine hybridoma cells was studied, In this study, Balb/c female mice were immunized with the purified viral protein of EMC-D virus and then fused with myeloma cells (NS-1). After cloning, monoclonal antidody-producing hybridoma cell lines were separated from non-producing hybridoma cell lines. Reverse transcriptase activity was checked in the supernate of monoclonal antibody-producing hybridoma clones, non-producins- hybridoma clones and myelorna cells as control. Monoclonal antibody-producing hybridoma cells shows statiscally significant higher activity as compare to that of non-producing hybridoma cells. The hybridoma cells which secrete high amount of reverss transcriptase shows significant number of extracellular C-type virus particles, In contrast, non-producing hybridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hybridoma cells release signifcant amount of reverse transcriptase and extracellular Otype virus particles, while non-producing hybridoma cells shows less release of reverse transcriptase and contains intracellular C-type virus particles.

Murine Sarcoma Virus로 변이시킨 가토신장세포의 지질조성에 대한 연구

정주광,박주홍,김우제,김경진,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

Differencies of lipid composition of mnrine sarcoma virus 970-17 traiisfomed rabbit kidney cells and their plasma membranes were studied. Free cholesterol was the major neutral lipid extracted from the whole cells and plasma membranes of all these cells. The micromoles of total neutral lipids and phospholipids are elevated in plasma membranes compared to whole cells from monolayer cultures. The distribution of fatty acids in the major lipid classes of plasma membranes and whole cells generally follow the composition of calf serum used in the culture media.

소배양법으로 배양한 생쥐췌장 베타세포의 형태학적 검정

유광성,전현우,박주홍,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

생쥐췌장 베타세포를 보다 순수하게 배양하기 위해 변형 고안한 소배양법을 검정하였다. 이 소배양법에 있어서는 96well plate에 islet cell을 이식하고 decantation한 다음 thimerosal에 노출시켜 많은 viable cell을 얻을 수 있었고, 생후 7일째의 생쥐의 췌장베타세포의 생존율이 가장 높았다. 또 이 소배양법으로 배양한 생쥐췌장 베타세포들은 위상차현미경, FITC-labelled anti-insulin antibody 염색 및 전자현미경으로 검정한 결과 형태학적으로 정상이었다. 기계적 선택방법으로서 생쥐췌장 베타세포의 decantation 최적시간은 세포를 이식한 후 12∼15시간 이었으며, 화학적 선택방법으로 0.6∼0.8㎍/㎖의 thimerosal에 노출시켜 fibroblastoid cell로 부터 생쥐췌장 베타세포의 분리를 증가시킬 수 있었으며, 두가지 방법을 동시에 응용하여 더 좋은 효율을 얻을 수 있었다. 또 이 소배양법에 있어서 cell duplication은 배양후 36시간후에 2배, 72시간후에 3배가 되었으며, 평균 generation시간은 46.8±7.0시간 이었다. 이들 결과로 미루어 보아 이소배양법은 생쥐췌장 베타세포 배양의 유용한 방법으로서, 췌장 베타세포에 연관된 각종 연구에 이용할 수 있을것으로 사료된다.

肝組織에서의 Glucagon의 脂肪酸合成 抑制機轉에 대한 硏究

曺義鉉,高光三 조선의대 1984 The Medical Journal of Chosun University Vol.9 No.1

食餌中 脂肪 蛋白質및 choline이 血淸과 肝組織중의 脂肪量變動에 미치는 影響

朴在允,高光三,金千圭 조선의대 1983 The Medical Journal of Chosun University Vol.8 No.1

Brevibacterium albidum중의 제한효소 Bal I의 정제 및 특성에 관한 연구

박정수,배광묵,박재윤,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

In this report, Bal I endonuclease from brevibacterium albium (ATCC 15831) strain was purified characterized. Bal I endonuclease was purified by the following procedures; ammonium sulfate fractionation, DEAE-cellulose column chromatography, phosphocellulose column chromatography. Bal I endonuclease has a specific site for type II enzyme in that has been proven by the facts that PBR322 DNA cleaved by Bal I endonuclease. All sited cleaved by Bal I are also cut by the speficif endonuclease Hae III from haemephilus aegyptiu. The recognition sequence of Bal I are. ◁수식 삽입▷ (원문을 참조하세요)