A Genetic Variant in MiR-146a Modifies Digestive System Cancer Risk: a Meta-analysis

Li, Ying-Jun,Zhang, Zhen-Yu,Mao, Ying-Ying,Jin, Ming-Juan,Jing, Fang-Yuan,Ye, Zhen-Hua,Chen, Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

MicroRNAs (miRNAs) negatively regulate gene expression and act as tumor suppressors or oncogenes in oncogenesis. The association between a single nucleotide polymorphism (SNP) in miR-146a rs2910164 and susceptibility to digestive system cancers was inconsistent in previous studies. In this study, we conducted a literature search of PubMed to identify all relevant studies published before August 31, 2013. A total of 21 independent case-control studies were included in this updated meta-analysis with 9,558 cases and 10,614 controls. We found that the miR-146a rs2910164 polymorphism was significantly associated with decreased risk of digestive system cancers in an allele model (OR=0.90, 95%CI 0.87-0.94), homozygote model (OR=0.84, 95%CI 0.77-0.91), dominant model (OR=0.90, 95%CI 0.84-0.96), and recessive model (OR=0.85, 95%CI 0.79-0.91), while in a heterozygous model (OR = 0.99, 95% CI 0.89-1.11) the association showed marginal significance. Subgroup analysis by cancer site revealed decreased risk in colorectal cancer above allele model (OR=0.90, 95%CI 0.83-0.97) and homozygote model (OR=0.85, 95%CI 0.72-1.00). Similarly, decreased cancer risk was observed when compared with allele model (OR=0.87, 95%CI 0.81-0.93) and recessive model (OR=0.81, 95%CI 0.72-0.90) in gastric cancer. When stratified by ethnicity, genotyping methods and quality score, decreased cancer risks were also observed. This current meta-analysis indicated that miR-146a rs2910164 polymorphism may decrease the susceptibility to digestive system cancers, especially in Asian populations.

Optimization Control Method of Intelligent Cooling and Lubrication for a Geared Spindle

Kun-Ying Li,Ping-Cheng Hsieh,Jen-Ji Wang,Shih-Jie Wei 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.10

The heat generated by the transmission system in the gear-driven spindle module of the machine tool not only influences the overall temperature field but also induces an excessive rise in temperature of gears tooth flank or rolling contact surface resulting in thermal deformation. In this study, high torque geared spindle was adopted to investigate a cooling control method, and an effective geared spindle cooling control method was proposed and tested. The controller of the cooling system received gear spindle load, rotational speed, and temperature of spindle to adjust the cooling flowrate, and the gas temperature of oil gas lubrication. Under spindle speed of 2000 rpm, the thermal deformations of X-axis, Y-axis, and Z-axis with cooling control are reduced by 43.7%, 46.8%, and 18.6%, respectively. The cooling control method not only can effectively control the rise in temperature of the meshing and contact friction area of the transmission system but also enables the transmission components and bearings of the geared spindle to achieve optimal cooling and lubrication effects.

<i>Yersinia pseudotuberculosis</i> Exploits CD209 Receptors for Promoting Host Dissemination and Infection

He, Ying-Xia,Ye, Cheng-Lin,Zhang, Pei,Li, Qiao,Park, Chae Gyu,Yang, Kun,Jiang, Ling-Yu,Lv, Yin,Ying, Xiao-Ling,Ding, Hong-Hui,Huang, Hong-Ping,Mambwe Tembo, John,Li, An-Yi,Cheng, Bing,Zhang, Shu-Sheng American Society for Microbiology 2019 Infection and immunity Vol.87 No.1

<P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals.</P><P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer’s patches to initiate dissemination. In this study, we demonstrate that <I>Y. pseudotuberculosis</I> utilizes its lipopolysaccharide (LPS) core to interact with CD209 receptors, leading to invasion of human dendritic cells (DCs) and murine macrophages. These <I>Y. pseudotuberculosis</I>-CD209 interactions result in bacterial dissemination to MLNs, spleens, and livers of both wild-type and Peyer’s patch-deficient mice. The blocking of the <I>Y. pseudotuberculosis</I>-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity. Based on the well-documented studies in which HIV-CD209 interaction leads to viral dissemination, we therefore propose an infection route for <I>Y. pseudotuberculosis</I> where this pathogen, after penetrating the intestinal mucosal membrane, hijacks the <I>Y. pseudotuberculosis</I>-CD209 interaction antigen-presenting cells to reach their target destinations, MLNs, spleens, and livers.</P>

rs12904 Polymorphism in the 3'UTR of EFNA1 is Associated with Colorectal Cancer Susceptibility in a Chinese Population

Mao, Ying-Ying,Jing, Fang-Yuan,Jin, Ming-Juan,Li, Ying-Jun,Ding, Ye,Guo, Jing,Wang, Fen-Juan,Jiang, Long-Fang,Chen, Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Accumulated evidence has indicated that Ephrin A1 (EFNA1) is associated with angiogenesis and tumorigenesis in various types of malignancies, including colorectal cancer (CRC). In the current study, we performed an online search using the public microarray database to investigate whether EFNA1 expression might be altered in CRC tissues. We then conducted a case-control study including 306 subjects (102 cases and 204 well-matched controls) in Xiaoshan County to assess any association between genetic polymorphisms in EFNA1 and CRC susceptibility. Searches in the Oncomine expression profiling database revealed EFNA1 to be overexpressed in CRC tissue compared with adjacent normal tissue. The rs12904 G-A variant located in the 3' untranslated region (UTR) of EFNA1 was observed to be associated with CRC susceptibility. Compared with the AA homozygous genotype, those carrying GA genotype had a decreased risk of developing CRC (odds ratio (OR)=0.469, 95% confidence interval (CI): 0.225-0.977, and P=0.043). The association was stronger among smokers and tea drinkers, however, no statistical evidence of interaction between rs12904 polymorphism and smoking or tea drinking on CRC risk was found. Our results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility. Larger studies and further mechanistic investigations are warranted to confirm our findings.

Triplet Platinum-based Combination Sequential Chemotherapy Improves Survival Outcome and Quality of Life of Advanced Non-small Cell Lung Cancer Patients

Chen, Li-Kun,Liang, Ying,Yang, Qun-Ying,Xu, Fei,Zhou, Ning-Ning,Xu, Guang-Chuan,Liu, Guo-Zhen,Wei, Wei-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Background: Maintenance chemotherapy is one strategy pursued in recent years with intent to break through the chemotherapy plateau for advanced non-small cell lung cancer (NSCLC). However, given the toxicity, platinum-based combinations are rarely given for this purpose. We carried out the present prospective study of triplet platinum-based combination sequential chemotherapy in advanced NSCLC to investigate if patients could tolerate and benefit from such intensive treatment. Methods: From Dec 2003 to Dec 2007, 190 stage IIIB and IV NSCLC patients in Sun yat-sen University sequentially received the 3 platinum-based combination (TP-NP-GP) treatment (T: paclitaxol175$mg/m^2$ d1; N: vinorelbine25$mg/m^2$ d1 and 8; G: gemcitabine1$g/m^2$ d1 and 8; P: cisplatin20$mg/m^2$ d1-5; repeated every 3 weeks). Patients were followed up to at least 3 years to obtain survival data. Treatment toxicities and the quality of life (QOL) were assessed during the whole treatment. Results: There were 187 patients evaluable. The TP, NP and GP response rates with sequential use were 42.8% (80/187), 41.1% (65/158) and 28.8% (21/73) respectively. Median survival time was 18.2 months and the 1, 2 and 3 year overall survival (OS) rates were 78.7%, 38.5% and 21.3%. Patients receiving > 6 cycles of chemotherapy had significantly longer OS and TTP (MST 25.3 vs. 14.5 months, TTP 15.1 vs. 9.1 months). The QOL on the whole for the patients was improved after chemotherapy. Conclusions: The sequential chemotherapy strategy with triplet platinum-based combination regimens can improve the survival outcome and the quality of life of advanced non-small cell lung cancer patients.

Platelet-derived exosomes promote the epithelial–mesenchymal transition in MCF7 cells

Li Mingying,Xin Ying,Liu Miao,Yu Kun 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1

Background Breast cancer is a common female malignancy. In recent years, the incidence of breast cancer has increased and has become much younger. Studies have shown that platelet-derived exosomes play an important role in the progression of cancer . Objective In this study, we investigated the effect of platelet-derived exosomes on the epithelial–mesenchymal transition (EMT), migration, and invasion of breast cancer cells. Activated and unactivated platelet-derived exosomes were extracted by ultracentrifugation. The morphology of platelet exosomes was observed and identifi ed by transmission electron microscopy and western blotting. After MCF7 cells are treated with platelet or exosomes, the mRNA and protein levels of EMT-related genes were detected by RT-PCR and western blotting, respectively, and the migration and invasion abilities of MCF7 cells were also observed by transwell. Results We observed some exosomes-like structures derived from activated platelets by transmission electron microscopy. The level of transforming growth factor-β in activated platelet-derived exosomes was higher than that in unactivated plateletderived exosomes. After activated platelet or activated platelet-derived exosomes were co-cultured with MCF7 cells, the expression of Snai1, N-cadherin, Vimentin and Fibronectin in activated platelets and breast cancer cells was significantly increased, while the expression of E-cadherin was significantly decreased. Activated platelets and activated platelet-derived exosomes significantly promoted the migration and invasiveness of MCF7 cells. These findings revealed that activated platelet or activated platelet-derived exosome enhances migration and invasion by promoting EMT in MCF cells. Conclusion In conclusion, platelet-derived exosomes initiate EMT and promote the migration and invasiveness of MCF7 cells.

항암작용에 대한 차폴리페놀의 생화학적 연구

Jen Kun Lin,I Ming Juan,Ying Ling Chen,Yu Chih Liang,Yu Li Lin 한국식품과학회 1995 국제녹차심포지엄 Vol.1995 No.1

Biochemical Studies on the Anti-Carcinogenesis of Tea Polyphenols

Jen-Kun Lin,I-Ming Juan,Ying-Ling Chen,Yu-Chih Liang,Yu-Li Lin 한국식품과학회 1995 한국식품과학회 심포지엄 Vol.1995 No.1

Extramedullary Plasmacytoma Involving the Bilateral Adrenal Glands on MR Imaging

Yuan Li,Ying-kun Guo,Zhi-gang Yang,En-sen Ma,Peng-qiu Min 대한영상의학회 2007 Korean Journal of Radiology Vol.8 No.3

We report here on a 64-year-old woman with extramedullary plasmacytoma involving the bilateral adrenal glands. Primary adrenal extramedullary plasmacytoma is extremely rare and only three cases of extramedullary plasmacytoma in the unilateral adrenal gland have currently been reported on. This case is of interest in that the bilateral adrenals were involved. In this article, we present the MRI findings and we briefly review the relevant literature.

RV-23, a Melittin-Related Peptide with Cell-Selective Antibacterial Activity and High Hemocompatibility

( Shi Kun Zhang ),( Qian Ma ),( Su Bo Li ),( Hong Wei Gao ),( Ying Xia Tan ),( Feng Gong ),( Shou Ping Ji ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6

RV-23 is a melittin-related antibacterial peptide (MRP) with lower cytotoxicity than either melittin or AR-23, another MRP. The aim of this study was to explore the mechanism of RV- 23`s antibacterial selectivity and its hemocompatibility. The results showed that all the peptides exhibited lytic activity against Staphylococcus aureus and Escherichia coli, with RV-23 showing the highest potency. Moreover, RV-23 had lower cytotoxicity than melittin or AR-23 at their minimal inhibitory concentration. In addition, CD experiments showed that melittin, RV-23, and AR-23 all had a typical α-helical structure, and RV-23 had the lowest α-helix content. The structural information showed that RV-23 has the lowest hydrophobicity and highest hydrophobic moment. Because hydrophobicity and α-helix content are believed to correlate with hemolysis, the results indicate that the selective lytic activity against bacteria of RV-23 may be due to its low hydrophobicity and α-helicity, which lead to low cytotoxicity without affecting antibacterial activity. Furthermore, RV-23 did not affect the structure and function of blood components such as red blood cells, platelets, albumin, and the blood coagulation system. In conclusion, RV-23 is a cell-selective antibacterial peptide with high hemocompatibility due to its unique structure.