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RISS 인기검색어
- 검색키워드
- 저자 : Krishnamurthi Kannan (검색결과 4 건)
- 무료
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Purification and Characterization of a Novel Plant-type Carbonic Anhydrase from Bacillus subtilis
Rishiram Ramanan,Krishnamurthi Kannan,Nadimuthu Vinayagamoorthy,Saravana Devi Sivanesan,Kunga Mohan Ramkumar,Tapan Chakrabarti 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.1
Carbonic anhydrase enzyme, one of the fastest known enzymes, remains largely unexplored in prokaryotes when compared to its mammalian counterparts despite its ubiquity. In this study, the enzyme has been purified from Bacillus subtilis SA3 using sequential Sephadex G-75 chromatography, DEAE cellulose chromatography, and sepharose-4B-L-tyrosine-sulphanilamide affinity chromatography and characterized to provide additional insights into its properties. The apparent molecular mass of carbonic anhydrase obtained by SDS-PAGE was found to be approximately 37 kDa. Isoelectric focusing of the purified enzyme revealed an isoelectric point (pI) of around 6.1 when compared with marker. The presence of metal ions such as Zn2+, Co2+, Cu2+, Fe3+, Mg2+, and anion SO4- increased enzyme activity while strong inhibition was observed in the presence of Hg2+, Cl-, HCO3-, and metal chelator EDTA. The optimum pH and temperature for the enzyme were found to be 8.3 and 37℃, respectively. Enzyme kinetics with p-nitrophenyl acetate as substrate at pH 8.3 and 37℃ determined the Vmax and Km values of the enzyme to be 714.28 μmol/mg protein/min and 9.09 mM, respectively. The Ki value for acetazolamide was 0.22 mM, compared to 0.099 mM for sulphanilamide. The results from N-terminal amino acid sequencing imply the purified protein is a putative beta-carbonic anhydrase with close similarities to CAs from plants, microorganisms.
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Narayanasamy Mathivanan,Gangadharan Surendiran,Krishnamurthy Srinivasan,Kannan Malarvizhi 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4
This study investigated the wound healing properties of the chloroform extract of Morinda pubescensfruit inrats. Topical application of M. pubescensfruit extract at 20 mg/mL and 10 mg/mL concentrations appreciably acceleratedwound healing in rats compared with controls. Complete wound contraction was achieved, and fresh hair began to grow inthe entire wounded area within 15 days in animals treated with the 20 mg/mL chloroform fruit extract of M. pubescenscom-pared with only 60% contraction without hair growth in control rats.
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Ramanan, Rishiram,Vinayagamoorthy, Nadimuthu,Sivanesan, Saravana Devi,Kannan, Krishnamurthi,Chakrabarti, Tapan The Korean Society of Phycology 2012 ALGAE Vol.27 No.4
Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) $CO_2$ concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric $CO_2$ concentration and the repression of CCM related proteins in the sample maintained at 10% $CO_2$. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important $C_i$ transporter proteins in the control sample of S. platensis, namely ferredoxin-$NADP^+$ reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low $CO_2$ concentrations and are not functional at high $CO_2$ concentration.
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Rishiram Ramanan,Nadimuthu Vinayagamoorthy,Saravana Devi Sivanesan,Krishnamurthi Kannan,Tapan Chakrabarti 한국조류학회I 2012 ALGAE Vol.27 No.4
Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%)CO2 concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric CO2 concentration and the repression of CCM related proteins in the sample maintained at 10% CO2. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important Ci transporter proteins in the control sample of S. platensis,namely ferredoxin-NADP+ reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently,this study conclusively proves that CCMs are only inducted at low CO2 concentrations and are not functional at high CO2concentration.
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