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Patil, Gajanan R.,Kokate, Vishwanath K. Korea Information Processing Society 2017 Journal of information processing systems Vol.13 No.4
A joint channel estimation and data detection technique for a multiple input multiple output (MIMO) wireless communication system is proposed. It combines the least square (LS) training based channel estimation (TBCE) scheme with sphere decoding. In this new approach, channel estimation is enhanced with the help of blind symbols, which are selected based on their correctness. The correctness is determined via sphere decoding. The performance of the new scheme is studied through simulation in terms of the bit error rate (BER). The results show that the proposed channel estimation has comparable performance and better computational complexity over the existing semi-blind channel estimation (SBCE) method.
( Gajanan R. Patil ),( Vishwanath K. Kokate ) 한국정보처리학회 2017 Journal of information processing systems Vol.13 No.4
A joint channel estimation and data detection technique for a multiple input multiple output (MIMO) wireless communication system is proposed. It combines the least square (LS) training based channel estimation (TBCE) scheme with sphere decoding. In this new approach, channel estimation is enhanced with the help of blind symbols, which are selected based on their correctness. The correctness is determined via sphere decoding. The performance of the new scheme is studied through simulation in terms of the bit error rate (BER). The results show that the proposed channel estimation has comparable performance and better computational complexity over the existing semi-blind channel estimation (SBCE) method.
Azadirachtin, a Novel Biopesticide from Cell Cultures of Azadirachta indica
Balaji Kaveti,Veeresham Ciddi,Srisilam Keshetty,Kokate Chandrakanth The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.2
Callus cultures of Azadirachta indica flower petals were established on MS medium supplemented with naphthalene acetic acid (1 mg/L), kinetin (0.5 mg/L) and sucrose $(3\%\;w/v)$. Cell cultures of Azadirachta indica were established and studied the growth and production kinetics. Half 85 medium supplemented with dicamba (2 mg/L), kinetin (1 mg/L) and sucrose $(3\%\;w/v)$ was found to be suitable for initiation and maintenance of cell cultures from the calli. MS medium supplemented with naphthalene acetic acid (NAA) (1 mg/L), kinetin (0.5 mg/L) and sucrose $(3\%\;w/v)$ was found to be suitable as production medium. Around $80\%\;(0.05\%\;w/v)$ of azadirachtin was found to be intracellular. The effect of various precursors, elicitors, permeabilizing agents and growth retardants in cell cultures was studied. The addition of precursors sodium acetate (10 mg/L), squalene (10 mg/L), isopentenyl pyrophosphate (1 mg/L) and geranyl pyrophosphate (1 mg/L) to the cell cultures on day 3 has shown significant increase in bioproduction of azadirachtin $(64.94{\pm}4.40\;mg/L,\;72.81{\pm}0.04\;mg/L,\;51.63{\pm}1.26\;mg/L\;and\;30.70{\pm}0.28\;mg/L\;respectively)$ over the control cultures $(4.70{\pm}0.27 mg/L)$. $5\%$ v/v cell extracts of Fusarium solani has shown moderate increase in the content of azadirachtin $(5.71{\pm}0.34\;mg/L)$ when compared to control cultures $(2.40{\pm}0.56\;mg/L)$. The addition of methyl jasmonate $(500\;{\mu}M/L)$ on day 3 has shown $\~4$ fold improvement in bioproduction of azadirachtln $(6.92{\pm}0.11\;mg/L)$ when compared to control cultures $(1.63{\pm}0.02\;mg/L)$. There was no significant effect of the studied growth retardants and permeabilizing agents on bioproduction of azadirachtin. Cells are cultivated in large volumes using the effective precursors.
C21 Pregnane Steroid from Caralluma umbellata (Asclepiadaceae)
Ramesh Mullangi,Bobbala Ravi Kumar,Chandrakanth Kokate,Sama Venkatesh,A.V.N. Appa Rao 한국생약학회 2005 Natural Product Sciences Vol.11 No.2
A C21 steroid has been isolated from the whole plants of Caralluma umbellata (Asclepiadaceae) andits structure was unambiguously asigned by spectroscopy.
Bhandari, Prerana,Ahmad, Firoz,Dalvi, Rupa,Koppaka, Neeraja,Kokate, Prajakta,Das, Bibhu Ranjan,Mandava, Swarna Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16
Background: Chromosomal aberrations identified in acute lymphoblastic leukemia (ALL) have an important role in disease diagnosis, prognosis and management. Information on karyotype and associated clinical parameters are essential to physicians for planning cancer control interventions in different geographical regions. Materials and Methods: In this study, we present the overall frequency and distribution patterns of chromosomal aberrations in both children and adult de novo B lineage ALL Indian patients using conventional cytogenetics, interphase FISH and multiplex RT-PCR. Results: Among the 215 subjects, cytogenetic results were achieved in 172 (80%) patients; normal karyotype represented 37.2% and abnormal 62.8% with a distribution as follows: 15.3% hypodiploidy; 10.3% hyperdiploidy; 15.8% t(9;22); 9.8% t(1;19); 3.7% t(12;21); 2.8% t(4;11); 2.8% complex karyotypes. Apart from these, we observed several novel, rare and common chromosomal rearrangements. Also, FISH studies using LSI extra-signal dual-color probes revealed additional structural or numerical changes. Conclusions: These results demonstrate cytogenetic heterogeneity of ALL and confirm that the incidence of chromosomal abnormalities varies considerably. To the best of our knowledge, this is one of the largest reported series of cytogenetic investigations in Indian B-lineage ALL cases. In addition, ongoing cytogenetic studies are warranted in larger groups of B-lineage ALL cases to identify newly acquired chromosomal abnormalities that may contribute to disease diagnosis and management.