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Rattanata, Narintorn,Klaynongsruang, Sompong,Daduang, Sakda,Tavichakorntrakool, Ratree,Limpaiboon, Temduang,Lekphrom, Ratsami,Boonsiri, Patcharee,Daduang, Jureerut Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3
Gallic acid was isolated from Caesalpinia mimosoides Lamk and the structure s identified based on spectroscopic analysis and comparison with authentic compound. In this study we compared the ability of natural gallic acid (nGA) and commercial gallic acid (cGA) to inhibit the proliferation of cholangiocarcinoma cell lines (M213, M214) and foodborne pathogenic bacteria (Salmonella spp. and Plesiomonas shigelloides). Both nGA and cGA had the same inhibitory effects on cell proliferation by inducing apoptosis of cholangiocarcinoma cell lines. In addition, nGA inhibited growth of foodborne pathogenic bacteria in the same manner as cGA. Our results suggest that nGA from Caesalpinia mimosoides Lamk is a potential anticancer and antibacterial compound. However, in vivo studies are needed to elucidate the specific mechanisms involved.
( Anawat Pakdeesuwan ),( Tomohiro Araki ),( Sakda Daduang ),( Wisarut Payoungkiattikun ),( Nisachon Jangpromma ),( Sompong Klaynongsruang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1
The hydrolysis of proteins constitutes an invaluable tool, granting access to a variety of peptide fragments with potentially interesting biological properties. Therefore, a hemoglobin (Hb) hydrolysate of Crocodylus siamensis was generated by digestion under acidic conditions. The antibacterial and antioxidant activities of the Hb hydrolysate were assessed in comparison with intact Hb. A disc diffusion assay revealed that the Hb hydrolysate exhibited antibacterial activity against eight strains of gram-positive bacteria and showed a higher efficacy than intact Hb. Moreover, the antioxidant activity of intact Hb and its hydrolysate was evaluated using ABTS and DPPH radical scavenging assays. The Hb hydrolysate exhibited free radical scavenging rates of 6-32%, whereas intact Hb showed a slightly higher activity. In addition, non-toxicity to human erythrocytes was observed after treatment with quantities of Hb hydrolysate up to 10 μg. Moreover, active fragmented Hb (P3) was obtained after purifying the Hb hydrolysate by reversed-phase HPLC. Scanning electron microscopy demonstrated the induction of bacterial cell membrane abnormalities after exposure to P3. Antibacterial and antioxidant activities play crucial roles for supporting the wound healing activity. Consequently, an in vivo mice excisional skin wound healing assay was carried out to investigate the effects of intact Hb treatment on wound healing in more detail. The results clearly demonstrate that intact Hb is capable of promoting 75% wound closure within 6 days. These findings imply that intact Hb of C. siamensis and its acid hydrolysate may serve as valuable precursors for food supplementary products benefitting human health.
Wisarut Payoungkiattikun,Anupong Joompang,Suyanee Thongchot,Boonpob Nowichai,Nisachon Jangpromma,Sompong Klaynongsruang 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.1
Although several explications of anti-inflammatory therapeutic substances for treating inflammatory-related diseases have been broadly discussed within the last few decades, peptide-based compounds display the potential to be novel inflammation treatment agents. Here, we evaluated the anti-inflammatory activity and other inflammationassociated activities, including anti-oxidative stress and anti-apoptosis properties, of the cationic peptides KT2 and RT2. Nitric oxide (NO) and other inflammatory markers were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells co-incubated with peptides. The levels of interrelated gene and protein expressions were quantified. Peptides formed complexes with LPS and displayed anti-inflammatory properties by reducing NO and pro-inflammatory cytokine production in inflamed RAW 264.7 cells. These peptides also exhibit a strong suppressive effect on mRNA expression levels of inducible nitric oxide synthase, tumor necrosis factor-α, signal transducer and activator of transcription 1, c-Jun N-terminal protein kinase (JNK)-1, nuclear factor kappa B (NF-κB), and p38 mitogen-activated protein kinase (MAPK), which affects the decay of phosphorylated JNK-1, p38 MAPK, and NF-κB p65 protein expression. Both peptides induce up-regulation of anti-inflammatory mRNA and protein expression levels of extracellular signal-regulated kinase and mRNA expression levels of MAPK phosphatase-1. Also, the production of reactive oxygen species was observed to be markedly reduced. Furthermore, peptides exhibited an anti-apoptotic property. To our knowledge, this is the first report of the multi-functional peptides KT2 and RT2 exerting broad biological activity related to anti-inflammatory effects. These peptides have potential for delivering a medical method for the handling of inflammation-related diseases.
In Vitro and in Vivo Wound Healing Properties of Plasma and Serum from Crocodylus siamensis Blood
( Nisachon Jangpromma ),( Sutthidech Preecharram ),( Thanawan Srilert ),( Surachai Maijaroen ),( Pramote Mahakunakorn ),( Natsajee Nualkaew ),( Sakda Daduang ),( Sompong Klaynongsruang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.
( Weeraya Phupiewkham ),( Qiumin Lu ),( Wisarut Payoungkiattikun ),( Threeranan Temsiripong ),( Nisachon Jangpromma ),( Ren Lai ),( Sompong Klaynongsruang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Leukocytes are reportedly the first line of the innate immune defense and essential for the control of common bacterial infections. Therefore, in this work, the antibacterial activity of crocodile leukocyte extract against Propionibacterium acnes was evaluated, and we also characterized the related activity of skin infection. The leukocyte extract showed the minimum inhibitory concentration to be 100 μg/ml to P. acnes. SEM imaging demonstrated that the leukocyte extract adversely affected P. acnes cell permeability in a concentration-dependent manner. Furthermore, the crocodile leukocyte extract could significantly reduce proinflammatory markers and decrease inflammatory signs in infected mouse ears. The crude leukocyte extract was further purified using FPLC and RP-HPLC. The resulting fraction F5 was indicated as the anti-acne peptide-containing fraction. The molecular mass of the peptide contained in F5 was calculated to be 4,790.5 Da. N-Terminal sequencing revealed the amino acid sequence as GPEPVPAIYQ, which displays similarities to immunoglobulin A and leucine-rich repeat neuronal protein. This is the first reported amino acid sequence of a crocodile leukocyte extract that possesses anti-acne activity. To attempt to use it in a prototype cosmetic, an anti-acne gel containing crude crocodile leukocyte extract was formulated, resulting in seven gel formulations (G1, G2, G3, G4, G5, G6, and G7). The formulations G5, G6, and G7 exhibited 2-fold higher anti-acne activity than G1-G4. Investigation of accelerating stability studies of anti-acne gel formulations G5, G6, and G7 demonstrated that a low storage temperature (4ºC) is suitable for maintaining the physical properties and biological activity of the anti-acne gel products.
Anupong Joompang,Preeyanan Anwised,Kunlathida Luangpraditkun,Nisachon Jangpromma,Jarupa Viyoch,Ce´line Viennet,Sompong Klaynongsruang 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.8
Ultraviolet (UV) radiation generates a range of biological effects in the skin, which includes premature skin aging, hyperpigmentation, and cancer. Therefore, the development of new effective agents for UV-related skin damage remains a challenge in the pharmaceutical industry. This study aims to test the inhibitory effect of crocodile white blood cell (cWBC) extract, a rich source of bioactive peptides, on ultraviolet B (UVB)-induced melanocyte pigmentation. The results showed that cWBC (6.25–400 μg/mL) could inhibit tyrosinase without adduct formation by 12.97 ± 4.20% on average. cWBC pretreatment (25–100 μg/mL) had no cytotoxicity and reduced intracellular melanin to 111.17 ± 5.20% compared with 124.87 ± 7.43 for UVB condition. The protective role of cWBC pretreatment against UVB was exhibited by the promotion of cell proliferation and the prevention of UVB-induced morphological change as observed from F actin staining. The decrease of microphthalmia-associated transcription factor expression levels after cWBC pretreatment might be a mechanism by which cWBC suppresses UVB-induced pigmentation. These results suggest that cWBC could be beneficial for the prevention of UVB-induced skin pigmentation.