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Mammalian Bax initiates plant cell death through organelle destruction
Yoshinaga, Keiko,Arimura, Shin-ich,Hirata, Aiko,Niwa, Yasuo,Yun, Dae-Jin,Tsutsumi, Nobuhiro,Uchimiya, Hirofumi,Yamada, Maki Kawai Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Mammalian Bax is known to cause cell death when expressed in plants. We examined transgenic plants expressing both Bax and oraganelle-targeted green fluorescent protein to determine the cellular changes that occur during Bax-induced cell death. The mitochondria changed morphologically from being bacilli-shaped to being round, eventually becoming swollen. Mitochondria streaming also stopped. The chloroplasts lost membrane function and their contents leaked out, followed by the disruption of the vacuole. Light was not essential for Bax-induced ion leakage or organelle disruption. These results indicate that Bax-induces temporal and spatial cell death events at the organelle level in the plant. A heterologous system, using Bax, would therefor be available to investigate cell death, which is commonly conserved in animals and plants
Development of Knee Joint Robot for Students Becoming Therapist
Yoshifumi Morita,Yuki Kawai,Yusuke Hayashi,Tatsuya Hirano,Hiroyuki Ukai,Kouji Sanaka,Hironori Nakamuta,Keiko Takao 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10
The authors are developing a knee joint robot as an educational tool for students becoming physical therapist (PT) or occupational therapist (OT). The robot can simulate some problems of a knee joint, such as range of motion trouble, contracture, rigidity, spasticity and so on. The robot has a knee joint mechanism to realize not only flexion/extension movement but also inner/outer rotation movement, which is based on the idea of rolling, sliding and coming off movement. In addition the robot has the wire drive system to control the knee joint passive movement. In this paper we design optimal arrangement of four pulleys in the wire drive system by introducing performance indices. In addition we design control algorithms to imitate two kinds of knee joint troubles and verify the effectiveness by fundamental experiments.
( Hitoshi Kuramae ),( Norio Tatsumi ),( Keiko Sugaki ),( Atsushi Naito ),( Hiroki Kawai ),( Ikuo Yamaguchi ),( Mitsuhiro Hori ),( Kazuhisa Inuzuka ) 대한임상병리사협회 2015 임상미생물검사학회 발표자료집 Vol.2015 No.-
Introduction Recently, several bacteria isolated in a clinical setting have drug-resistance, which has become a serious problem. Particularly, extended-spectrum β- lactamase (ESBL) producing Enterobacteriaceae(ESBLPE) have spread not only in-hospital infection but also community-acquired infection. In 2006, we established a meeting which is called "Mikawan-kai" for the purpose of establishment and the standardization of the method for detection criteria of ESBLPE with 17 institutions in east area of Aichi prefecture. We report about the situation and trend of ESBLPE detection in this area. Materials and Methods This study was carried out by 7 institutions (190-836 bed) in east area of Aichi prefecture from January to December in 2013. The judgment of ESBLPE was conducted by the routine method in each institution. Results The range of detection rates identified or suspected ESBLPE were E. coli : 8-13%, K. pneumoniae : 3-6%, K. oxytoca : 0-17%, and P. mirabilis : 6-22%, respectively. And the results of antimicrobial susceptibility tests of E. coli showed that the rates of resistance to CPDX, CTX, and CAZ, which were recommended as a screening of identification for ESBLPE, were 12-18%, 8-13%, and 2-6%. Discussion and conclusion The highest frequency of detection of ESBLPE was E. coli, which was about 10% in each institution and has been increasing as compared with that of previous research in 2009 (4-9%). The results of ESBLPE detection in K. oxytoca and P. mirabilis showed some differences among institutions. Resistance rate for antimicrobial drug seems to be higher in CPDX, which is lower in CAZ. The detection rate of ESBLPE has not steeply increased for recent 5 years, whereas it still accounts for a high rate. For the prevention to further expansion of ESBLPE in this area, we intend to develop our activity through standardization of detection methods and criterion of identification for ESBLPE and share information.