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Kazutoshi Hamada,Kazuhiko Shimasaki,Yasuyo Nishimura,Haruka Egawa,Katsuhira Yoshida 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.4
We examined the effects of spectral composition conversion films on proliferation of protocorm-like body (PLB) of Cymbidium finlaysonianum. In this study, several red fluorescent films (NB-PS, BD-PS, CD-PC and ID-PS) were used for culture. These films, which developed by our group, have different characteristics on the pattern of absorption and emission spectra of the light. PLBs were cultured under transmitted light from these films provided from cool fluorescent light with 16h photoperiod for 40 days. Blank film (transparent PS film) was covered as the control. NB-PS and BD-PS films increased significantly the number of PLB after 20 days of culture. However, the number of PLB covered with ID-PS and CD-PC film was almost similar to blank film throughout the culture. At 40 days after culture, the increase of number of PLB was as follows; BD-PS>NB-PS>CD-PC>ID-PS>Blank. Also, NB-PS and BD-PS film increased significantly the normal PLBs. In addition, the number of PLB was almost similar regardless the period of the NB-PS film-covered time. These results showed that the proliferation of Cymbidium PLB would be controlled accurately by a delicate difference of the wavelength distribution of the light. We found that the red fluorescent films (NB-PS and BD-PS) increased the PLB proliferation of C. finlaysonianum, even if the film was covered only during early stage of the culture.