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      • KCI등재

        Transcriptome deep sequencing, identification of novel microRNAs and validation under drought stress in turmeric (Curcuma longa L.)

        R. Santhi,T. E. Sheeja,K. S. Krishnamurthy 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.4

        microRNAs (miRNAs) are small non-coding RNAs that execute regulation of gene expression at the post-transcriptional level. Information on turmeric miRNAs is very rare. In the present study, we have identified 27 conserved and 40 non-conserved miRNA families by high-throughput Illumina sequencing and downstream in silico analysis. We could also identify 97 novel candidate miRNAs specific to turmeric. Selected miRNAs were validated by stem-loop RT-PCR and northern blotting. The targets were predicted for identified miRNAs and were found to be involved in plant growth and development, stress response and metabolism. Expression pattern of selected conserved miRNAs in response to drought stress was analyzed by stem-loop qRT-PCR. This is the first report on miRNA content of turmeric transcriptome, which might serve as a foundation for more future work on miRNA based gene regulation in this medicinally important spice crop.

      • KCI등재

        Assessment of Cyclic Load Induced Energy Dissipation and Damping on GFRP Composite Laminate

        T. G. Loganathan,K. Vinoth Kumar,R. Krishnamurthy 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

        Polymeric composites exhibit load sensitive stiffness unlike the case of homogeneous metallic material. Composites are widely used in dynamic loading environment and hence it is necessary to study their response in terms ofstructural properties. Behavioural changes of glass epoxy composite laminate on exposure to cyclic loading has been assessedin terms of energy dissipation (Ed) and Damping factor (DF) by hysteresis loop. GFRP composite specimens (UD-0, 0/30/60/0, 0/45/0/-45, 0/90/90/0, and 0/90/0/90) are exposed to low velocity constant amplitude cyclic loading using a laboratoryarrangement (by an eccentric disc) at 4.6 Hz and 8.6 Hz frequencies. In fibre-reinforced composites apart from the fibrevolume fraction, the fibre interaction angle significantly influences their dynamic properties on loading. Unidirectional (UD-0) laminate exhibits low damping/energy dissipation, while 0/90/0/90 laminate with large fibre interaction angle showshighest damping/energy dissipation. Whereas, symmetric cross ply (0/90/90/0) laminate acts as a performance demarcationamong the chosen laminates. Thus, optimum Ed/DF properties of GFRP laminate in dynamic environment is attributed tosymmetric lay-up, smaller fibre orientation interaction angle in the lay-up sequence and 0 fibre layer at the boundary.

      • KCI등재

        Significance of cyclic loading parameters on the flexural response of the GFRP composites

        T. G. Loganathan,R. Krishnamurthy,K. Chandrasekaran 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.7

        Automotive design calls for weight reduction, considering fuel consumption and vehicle dynamics as well. Application of composite materials for suspensions and other structural members is in that direction. Owing to their high specific strength and inherent structural damping qualities, composites can be an alternative to metallic materials, especially with the suspension-springs. Further, epoxy composites can resist environmental influences, facilitating easier maintenance. In this study, we assessed the flexure response of GFRP composite exposed to pre flexure cyclic loading. This was to simulate the loading environment of an automobile leaf spring. Experiments were conducted with Taguchi’s DOE using L9 orthogonal array with two factors and three levels. The test specimens were prepared by hand lay-up technique with four layers of (0/0/0/0, 0/30/60/0, 0/90/90/0 and 0/45/0/-45) orientation. Constant peak amplitude cyclic loading was imposed on the specimen by an eccentric disc in a radial drilling machine. The flexural modulus of the cyclic loaded specimen was obtained by three point bend test as per ASTM D790. Assessment of the test results by ANOVA, S/N ratio and regression analysis indicated that the flexural modulus of the pre flexural cyclic loaded composite is significantly influenced by the loading parameters (loading cycle and frequency), and the flexural modulus can be enhanced with reduced fiber orientation interactions within the lay-up at critical operating conditions. The damage pattern influenced by fibre orientation in the lay-up sequence is ascertained by SEM images.

      • KCI등재

        Catalytic Composites Based on Yttria Stabilized Zirconia for Oxidative Dehydrogenation of Ethane

        김종남,조순행,Jung-Il Yang,K. R. Krishnamurthy 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.2

        LiCl/YSZ is found to be a very effective catalyst for the oxidative dehydrogenation of ethane. LiCl supported on YSZ-MgO composite shows increase in catalytic activity and ethylene selectivity. Addition of Mn and Sn as promoters to this system leads to 85% ethane conversion, 77% ethylene selectivity and 65% ethylene yield at 662 oC. Use of Li2O in the place of LiCl results in lower ethylene yields. Further modification is needed to improve the catalyst stability.

      • SCIESCOPUSKCI등재

        Catalytic Composites Based on Yttria Stabilized Zirconia for Oxidative Dehydrogenation of Ethane

        Yang, Jung-Il,Kim, Jong-Nam,Cho, Soon-Haeng,Krishnamurthy, K. R. 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.2

        LiCI/YSZ is found to be a very effective catalyst for the oxidative dehydrogenation of ethane. LiCI supported on YSZ-MgO composite shows increase in catalytic activity and ethylene selectivity. Addition of Mn and Sn as promoters to this system leads to 85% ethane conversion, 77% ethylene selectivity and 65% ethylene yield at 662℃. Use of Li_(2)O in the place of LiCI results in lower ethylene yields. Further modification is needed to improve the catalyst stability.

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