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Saccharomyces cerevisiae에서 myo-Inositor 결핍에 의한 Respiratory Capacity의 감소
정경환,이준식 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
Saccharomyces cerevisiad (S. cerevisiae)의 growth factor중의 하나인 myo-Inositol은 membrane-associated 효소인 phosphatidylinositol 합성효소에 의해 세포막의 구성 성분인 phosphatidylinositol로 incorporation 되는 것으로 알려져있다. 그래서 myo-inositol이 결핍되면 막구조와 기능에 좋지 않은 영향을 주는 것으로 알려져 있다. 이러한 myo-inositol의 생화학적 기능을 근거로 myo-inositol의 결핍이 호기적 포도당 대사에 미치는 영향을 조사하기 위하여 S. cerevisiae의 respiratory capacity를 대변하는 인자인 specific oxygen uptake rate (Q_O2)를 회분배양과 연속배양을 이용하여 측정하였다. 그리고, S. cerevisiae의 호기적 포도당 대상의 respiratory capacity를 myo-inositol이 결핍된 경우와 그렇지 않은 경우에 연속배양에서 포도당 pulse-addition을 한 후 관찰하였다. 그 결과 회분배양과 연속배양에서 myo-inositol이 결핍된 경우 maximum specific oxygen uptake rate (Q_O2. max)이 감소되는 현상을 관찰하였고, 포도당 pulse-addition 실험에서 얻은 결과를 정량적으로 분석하여 본 결과 myo-inositol의 결핍이 respiratory capacity의 감소를 초래하는 것이 관찰되었다. myo-Inositol, a growth factor for Saccharomyces cerevisiad (S. cerevisiae), has been know to be incorporated into phosphatidylinositol (PI), which is a kind of phospholipid in the cell membrane, by a membrane-associated PI-synthesizing enzyme. The deficiency of myo-inositol in S. cerevisiae adversely affected the membrane structure and function. On the basis of biochemical functions of myo-inositol, the effect of deficiency of myo-inositol on the aerobic glucose metabolism was investigated by measuring specific oxygen uptake rate(Q_O2) used as an indicator representing the respiratory capacity of S. cerevisiae in batch and continuous cultures. The respiratory capacity of aerobic glucose metabolism in S cerevisiae was also monitored after glucose pulse-addition in a continuous culture (D=0.2, 1/hr), in which glucose was utilized through respiratory metabolism. The deficiency of myo-inositol was found to lead to both the decrease of the maximum specific oxygen uptake rate (Q_O2. max) observed from the batch as well as in the continuous culture experiment and the decrease of the respiratory capacity of aerobic glucose metabolism of S. cerevisiae determined from the glucose pulse-addition experiment, in which the glucose flux into respiratory and fermentative metabolism was quantitatively analyzed.
The Double-Layered Microsphere: Encapsulation of Water-Soluble Protein with PLGA
Ko, Jong Tae,Jung, Hyung Joon,Mo, Jong Hyon,Cho, Jae Song,Yuk, Soon Hong,Shin, Hyung Shik,Kim, Moon Suk,Rhee, John M.,Lee, Hai Bang,Khang, Gil Son Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.342-343 No.-
<P>The double-layered microspheres play an important role in controlling drug delivery for pharmaceutical application, because of the low initial burst compared with single-layered spheres and targetable delivery to specific organ. But it has drawback in loading drug and controlling size. In this study, we developed double-layered spheres using relatively simple oil-in-water (O/W) solvent evaporation method using bovine serum albumin (BSA) as water-soluble protein and poly(D,L-lactide-co-glycolide) (PLGA). BSA/PLGA double-layered microspheres were fabricated using O/W solvent evaporation method and investigated the specific character of double-layered microspheres according to the kind of surfactants. In SEM observation, double layered microsphere had spherical shape and smooth surface without pores. And the double layered microsphere using O/W solvent evaporation method was transparency because of slow evaporation of solvent. In fluorescent observation, we observed the fluorescent core in the double-walled spheres composed of FITC-BSA and PLGA using fluorescent observation. In the case of polyvinylalcohol as emulsifier, the yield was better than gelatin. As decreased concentration of PLGA, the size of double-layered microspheres deceased.</P>