Construction of Recombinant Pichia pastoris Carrying a Constitutive AvBD9 Gene and Analysis of Its Activity

( Jian Tu ),( Kezong Qi ),( Ting Xue ),( Haiting Wei ),( Yongzheng Zhang ),( Yanli Wu ),( Xiuhong Zhou ),( Xiaolong Lv ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. Using the preferred codon of Pichia pastoris, the mature AvBD9 peptide was designed and synthesized, based on the sequence from GenBank. The P. pastoris constitutive expression vector pGHKα was used to construct a pGHKα-AvBD9 recombinant plasmid. Restriction enzyme digestion was performed using SacI and BglII to remove the ampicillin resistance gene, and the plasmid was electrotransformed into P. pastoris GS115. High-expression strains with G418 resistance were screened, and the culture supernatant was analyzed by Tricine-SDS-PAGE and western blot assay to identify target bands of about 6 kDa. A concentrate of the supernatant containing AvBD9 was used for determination of antimicrobial activity. The supernatant concentrate was effective against Escherichia coli, Salmonella paratyphi, Salmonella pullorum, Pseudomonas aeruginosa, Enterococcus faecalis, and Enterobacter cloacae. The fermentation product of P. pastoris carrying the recombinant AvBD9 plasmid was adjusted to 1.0 × 108 CFU/ml and added to the drinking water of white feather broilers at different concentrations. The daily average weight gain and immune organ indices in broilers older than 7 days were significantly improved by the AvBD9 treatment.

Comparison of the efficacy and safety of transurethral laser versus open prostatectomy for patients with large-sized benign prostatic hyperplasia: A meta-analysis of comparative trials

Hai-bin Wei,Bing-yi Guo,Yao-fen Tu,Xuan-han Hu,Wei Zheng,Da-hong Zhang,Jian Zhuo 대한비뇨의학회 2022 Investigative and Clinical Urology Vol.63 No.3

Purpose: The selection of open prostatectomy (OP) over transurethral laser surgery is controversial for large volume prostates. Thus, we aim to compare the efficacy and safety of transurethral laser versus OP, and provide the latest evidence of clinical practice for large-sized benign prostatic hyperplasia (BPH). Materials and Methods: This meta-analysis used Review Manager V5.3 software and the systematic literature search of Cochrane Library, Embase, PubMed, and Web of Science datasets was performed for citations published from 2000 to 2020 that compared transurethral laser with OP for the treatment of large BPH. Variables of interest assessing the two techniques included clinical characteristics, and the perioperation-, effectiveness-, and complication-related outcomes. Results: The meta-analysis included twelve studies containing 1,514 patients, with 792 laser and 722 OP. The transurethral laser group was associated with shorter hospital stay and catheterization duration, and less hemoglobin decreased in the perioperative variables. There was no significant difference in the international prostate symptom score, post-void residual urine volume, maximum flow rate, and quality of life score. Transurethral laser group had a significantly lower incidence of blood transfusion than OP group (odds ratio, 0.10; 95% confidence interval, 0.05 to 0.19; p<0.001; I2=8%), and no statistical differences were found with respect to the other complications. Conclusions: Both OP and transurethral laser prostatectomy are effective and safe treatments for large prostate adenomas. With these advantages of less blood loss and transfusion, and shorter catheterization time and hospital stay, laser may be a better choice for large BPH.

A Kalman filter based algorithm for wind load estimation on high-rise buildings

Lun-hai Zhi,Pan Yu,Jian-wei Tu,Bo Chen,Yong-gui Li 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.64 No.4

High-rise buildings are generally sensitive to strong winds. The evaluation of wind loads for the structural design, structural health monitoring (SHM), and vibration control of high-rise buildings is of primary importance. Nevertheless, it is difficult or even infeasible to measure the wind loads on an existing building directly. In this regard, a new inverse method for evaluating wind loads on high-rise buildings is developed in this study based on a discrete-time Kalman filter. The unknown structural responses are identified in conjunction with the wind loads on the basis of limited structural response measurements. The algorithm is applicable for estimating wind loads using different types of wind-induced response. The performance of the method is comprehensively investigated based on wind tunnel testing results of two high-rise buildings with typical external shapes. The stability of the proposed algorithm is evaluated. Furthermore, the effects of crucial factors such as cross-section shapes of building, the wind-induced response type, errors of structural modal parameters, covariance matrix of noise, noise levels in the response measurements and number of vibration modes on the identification accuracy are examined through a detailed parametric study. The research outputs of the proposed study will provide valuable information to enhance our understanding of the effects of wind on high-rise buildings and improve codes of practice.

The Classic Security Application in M2M: the Authentication Scheme of Mobile Payment

( Liang Hu ),( Ling Chi ),( Hong-tu Li ),( Wei Yuan ),( Yu Yu Sun ),( Jian-feng Chu ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

As one of the four basic technologies of IOT (Internet of Things), M2M technology whose advance could influence on the technology of Internet of Things has a rapid development. Mobile Payment is one of the most widespread applications in M2M. Due to applying wireless network in Mobile Payment, the security issues based on wireless network have to be solved. The technologies applied in solutions generally include two sorts, encryption mechanism and authentication mechanism, the focus in this paper is the authentication mechanism of Mobile Payment. In this paper, we consider that there are four vital things in the authentication mechanism of Mobile Payment: two-way authentication, re-authentication, roaming authentication and inside authentication. Two-way authentication is to make the mobile device and the center system trust each other, and two-way authentication is the foundation of the other three. Re-authentication is to re-establish the active communication after the mobile subscriber changes his point of attachment to the network. Inside authentication is to prevent the attacker from obtaining the privacy via attacking the mobile device if the attacker captures the mobile device. Roaming authentication is to prove the mobile subscriber`s legitimate identity to the foreign agency when he roams into a foreign place, and roaming authentication can be regarded as the integration of the above three. After making a simulation of our proposed authentication mechanism and analyzing the existed schemes, we summarize that the authentication mechanism based on the mentioned above in this paper and the encryption mechanism establish the integrate security framework of Mobile Payment together. This makes the parties of Mobile Payment apply the services which Mobile Payment provides credibly.

Effect of Contact Pressure on Reciprocating Wear Behavior of PEEK, PTFE, and UHMWPE

Liu Lian,Duan Haitao,Jia Dan,Tu Jiesong,Zhan Shengpeng,Li Yinhua,Luo Xiaoshuang,Zhan Wen,Xiong Wei,Li Jian 한국고분자학회 2020 폴리머 Vol.44 No.6

Engineering plastics are macromolecular compounds composed of covalently bonded macromolecules, which have been widely used in sliding wear-resistance materials in isolation bearings. In this study, an MFT-5000 reciprocating friction testing machine was used to compare the friction and wear performance of polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), and ultra-high molecular weight polyethylene (UHMWPE) under heavy load conditions in dry friction condition. The results show that load has a significant effect on the friction coefficient, wear rate, and wear mechanism of three materials. The instant friction coefficient of PTFE fluctuates under high load, the wear rate clearly increases with the increase in load. Therefore, the application under high load conditions is limited. The wear rate of UHMWPE is the least affected by the load among the three materials. Even when the load exceeds the yield strength, the wear resistance is still good. The friction coefficient of PEEK decreases with the increase in load but maintains a high value that restricts its application in sliding friction pair materials to some degree.