Production of 3-hydroxypropionic acid by recombinant Klebsiella pneumoniae based on aeration and ORP controlled strategy

Jian-Guo Zhu,Xiao-Jun Ji,Jun Du,Shuang Li,Yue-Yue Ding,He Huang 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.6

A biosynthetic pathway for the production of 3-hydroxypropionic acid (3-HP) from glycerol was established in recombinant Klebsiella pneumoniae by introducing the aldehyde dehydrogenase gene from Escherichia coli. The activity of aldehyde dehydrogenase, which oxidized 3-hydroxypropionaldehyde (3-HPA) to 3-HP, was detected and 3-HP was produced by the recombinant strains. Three different oxygen supply strategies, associated with measuring the oxidoreduction potential (ORP) during the fermentation under these conditions, were adopted for higher production of 3-HP by the recombinant cells. About 0.8 g/l 3-HP and more 1,3-propanediol production by the recombinant Klebsiella pneumoniae were obtained under completely aerobic conditions. Under micro-aerobic conditions, 3-HP production could be increased to 2.2 g/l and 1,3-propanediol production was almost the same as in the original strain. Under the anaerobic conditions, 1,3-propanediol was the main product and about 1.3 g/l 3-HP was produced. Finally, 3-HP production of the recombinant strain was increased to 2.8 g/l under micro-aerobic condition with a further two-stage ORP controlled strategy.

Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus

Du, Meng-Fang,Yin, Xin-Ming,Guo, Zhong-Jian,Zhu, Liang-Jun Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6

Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.

Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells

Du, Ze-Peng,Wu, Bing-Li,Xie, Jian-Jun,Lin, Xuan-Hao,Qiu, Xiao-Yang,Zhan, Xiao-Fen,Wang, Shao-Hong,Shen, Jin-Hui,Li, En-Min,Xu, Li-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.

Bifurcations of A Third-Order Common-Base Transistor Circuit

Jian Jun Hou,Jing Tang Wu,Xi Yu Du 대한전자공학회 1992 대한전자공학회 Conference Vol.1992 No.2

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin, Jun-Jie,Wang, Jun-Mei,Du, Jiang,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin- suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

Trans-arterial Chemo-Embolization in Treating Elderly Patients with Hepatocellular Carcinoma

Zhang, Jian-Feng,Liu, Jun-Mao,Zhang, Nin,Du, Chao,Zheng, Qin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: This analysis was conducted to evaluate the efficacy and safety of Trans-arterial Chemo-Embolization (TACE) in treating Elderly patients with Hepatocellular Carcinoma (EHPC). Methods: Clinical studies evaluating the efficacy and safety of TACE on response and safety for patients with EHPC were identified by using a predefined search strategy. Pooled response rate of treatment were calculated. Results: In TACE based regimen, clinical studies which including patients with EHPC were considered eligible for the evaluation of response. And, in these TACE based treatments, pooled analysis suggested that, in all 288 patients whose response could be assessed, the pooled reponse rate was 29.5%(85/288) in TACE based treatment. The most commonly encountered TACE-related morbidity was liver function impairment. No grade III or IV renal or liver toxicity were observed. No treatment related death occurred in EHPC patients with TACE based treatments. Conclusion: This evidence based analysis suggests that TACE based treatments are associated with mild response rate and accepted toxicities for treating patients with EHPC.

Prognostic Factors Influencing Clinical Outcomes of Malignant Glioblastoma Multiforme: Clinical, Immunophenotypic, and Fluorescence in Situ Hybridization Findings for 1p19q in 816 Chinese Cases

Qin, Jun-Jie,Liu, Zhao-Xia,Wang, Jun-Mei,Du, Jiang,Xu, Li,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Malignant glioblastoma multiforme (GBM) is the most malignant brain tumor and despite recent advances in diagnostics and treatment prognosis remains poor. In this retrospective study, we assessed the clinical and radiological parameters, as well as fluorescence in situ hybridization (FISH) of 1p19q deletion, in a series of cases. A total of 816 patients with GBM who received surgery and radiation between January 2010 and May 2014 were included in this study. Kaplan-Meier survival analysis and Cox regression analysis were used to find the factors independently influencing patient progression free survival (PFS) and overall survival (OS). Age at diagnosis, preoperative Karnofsky Performance Scale (KPS) score, KPS score change at 2 weeks after operation, neurological deficit symptoms, tumor resection extent, maximal tumor diameter, involvement of eloquent cortex or deep structure, involvement of brain lobe, Ki-67 and MMP9 expression level and adjuvant chemotherapy were statistically significant factors (p<0.05) for both PFS and OS in the univariate analysis. Cox proportional hazards modeling revealed that age ${\leq}50$ years, preoperative KPS score ${\geq}80$, KPS score change after operation ${\geq}0$, involvement of single frontal lobe, deep structure involvement, low Ki-67 and MMP9 expression and adjuvant chemotherapy were independent favorable factors (p<0.05) for patient clinical outcomes.

Bifurcations of A Third-Order Common-Base Transistor Circuit

Hou, Jian Jun,Wu, Jing Tang,Du, Xi Yu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

This paper reports the dynamics and a great variety of one parameter bifurcation phenomena observed in the laboratory and confirmed by extensive computer simulations for the circuit reported firstly in [2]. The circuit consists of five elements, where the only nonlinear element is a common-base transistor pair with the characteristics of a current-controlled nonlinear resistor. Several strange attractors, the intermittent attractor, periodic windows, cascades of period doubling, period plus two and period minus one phenomena are observed by adjusting the value of L₁. The computer simulation results agree with the experiment results quite nicely. Hopf bifurcation is proved mathematically and the Lyapunov dimensions of several chaotic attractors are derived to confirm the existence of chaos.

Novel Auto-regressive Measurement of Diamond Tool Wear in Ultra-precision Raster Milling

Shao-Jian Zhang,Suet To,Chi-Fai Cheung,Jian-Jun Du 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.

In this paper, a new auto-regressive algorithm is proposed to reconstruct 3D topographic surface for diamond tool wear, using its in-process image. First, based on digital image processing technique, tool wear lands are separated from a tool wear image captured by a CCD camera under a 100X optical system; Second, a traverse search method of arc translation is put forward to eliminate feigned wear lands, and a least square polynomial method is adopted to fit inner- and outer-contours of the wear lands, self-adaptively filtering noises and connecting the discontinuous wear lands; Finally, the auto-regressive calibration method is developed to reconstruct its 3D topographic surface. The wear land is extracted self-adaptively, and the wear area, maximal wear width, average wear width and worn volume can be determined automatically by the algorithm. The reconstructed 3D topography of the tool wear land can be identified, based on the tool wear image captured by SEM. The result indicates that the method is capable of reconstructing 3D topography of the tool wear land and provides a possibility for in-process 3D-wear measurement in ultra-precision raster milling (UPRM) and the algorithm reliability is validated finally. And the influence of tool wear on surface roughness is discussed.

Influence of Culture Modes on the Microbial Production of Hyaluronic Acid by Streptococcus zooepidemicus

Long Liu,Jian Chen,Miao Wang,Jun Sun,Guocheng Du 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.3

The principal objective of this study was to assess the effects of culture modes including batch culture, pulse fed-batch culture, constant feeding rate fed-batch culture, and exponential fed-batch culture on the production of hyaluronic acid (HA) by Streptococcus zooepidemicus. Batch cultures had the highest levels of HA productivity, whereas fed-batch cultures were more favorable with regard to cell growth, and exponential fed-batch cultures evidenced the highest cell concentrations. A two-step culture model was proposed to enhance HA production: an exponential fed-batch culture was conducted prior to 8 h and then sucrose supplementation was applied for 8 h to start the batch fermentation of S. zooepidemicus. HA production and productivity were increased by 36 and 37% in the proposed two-step culture process as compared with that observed in the batch culture, respectively. The proposed two-step culture model can be applied in the production of secondary metabolites, and particularly of the exopolysaccharides.