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Jae Bok Yoon(윤재복),Jae Wahng Do(도재왕),Sang Hoon Kim(김상훈),Hyo Guen Park(박효근) 한국원예학회 2009 원예과학기술지 Vol.27 No.1
지금까지 Colletotrichum acutatum에 의해서 발생하는 고추(Capsicum spp.) 탄저병에 대한 안정적인 저항성 유전자원은 근연종인 Capsicum baccatum과 C. chinense에서만 동정되었다. C. baccatum var. PBC81의 탄저병 저항성을 C. annuum var. Matikas로 도입하기 위한 종간 교잡 및 배구제 방법이 사용되었다. 종간 교잡 F₁ 식물체들은 모두 완전한 화분 불임이어서 재배종인 ‘Matikas’를 화분친으로 사용하여 집중적인 여교잡을 수행한 결과 BC₁F₁ 집단을 육성할 수 있었다. 얻어진 수백 개의 BC₁F₁ 개체 중에서 탄저병 접종에 사용할 수 있는 다수의 과실이 달린 88개체만이 탄저병 저항성 유전 분석에 사용되었다. BC₁F₁ 집단의 저항성과 이병성의 분리비는 1:1 또는 3:1의 멘델식 분리비에 적합하지 않았으며, 집단의 빈도 분포는 저항성에서 이병성으로 연속분포를 보였다. 탄저병 저항성에 대한 광의의 유전력 측정을 위하여 selection differential(i)과 genetic gain(ΔG)을 이용하였고 공식 h²= ΔG/i에 의해 추정한 유전력은 약 0.61로 측정되었다. 이러한 결과는 C. baccatum var. PBC81의 탄저병 저항성이 몇 개의 유전자에 의해서 조절되는지는 알 수 없지만 다수의 유전자가 관여하는 양적유전형질이라는 것을 나타내는 것이다. Reliable genetic resources resistant to anthracnose, caused by Colletotrichum acutatum, in Capsicum have only been found in certain Capsicum species, e.g., Capsicum baccatum and C. chinense. Interspecific hybridization using embryo rescue was used to introgress anthracnose resistance from C. baccatum var. PBC81 into C. annuum var. Matikas. Because the interspecific hybrids were completely pollen-sterile, an interspecific BC₁F₁ as the first segregating population was raised through intensive backcrosses in which Matikas was used as the male parent. Among the hundreds of BC₁F₁ progeny, 88 plants setting many fruits were used to study inheritance of anthracnose resistance. The segregation of resistance to susceptibility in the BC₁F₁ population did not fit expected Mendelian ratios such as 1:1 or 3:1, and the frequency distribution of progenies varied continuously from resistant to susceptible. To estimate broad sense heritability of resistance, we used a real method of selection differential (i) and genetic gain (ΔG). According to the equation h²= ΔG/i, heritability was estimated as about 0.61. This result suggests that resistance of C. baccatum var. PBC81 can be controlled quantitatively, although we do not know yet how many genes are associated with the resistance.
Interspecific Cross Compatibility among Five Domesticated Species of Capsicum Genus
Jae Bok Yoon,Jae Wahng Do,Dong Cheol Yang,Hyo Guen Park 한국원예학회 2004 Horticulture, Environment, and Biotechnology Vol.45 No.6
The Capsicum genus includes 27 species; 5 domesticated and 22 wild ones. Among the 5 domesticated species, C. annuum is the most widely cultivated commercial species and thus, intensive breeding programs have been being carried out to improve this species worldwide. Recently, it has been reported that almost all traits needed for pepper breeding are mainly found in related or wild species of cultivated pepper (C. annuum). This experiment was carried out to determine interspecific crossability among 13 different genotypes of 5 cultivated species. The crosses of C. annuum with C. chinense or with C. frutescens were compatible or partial-compatible in many cases, although there were some differences in crossability among genotypes within the two parental species. In contrast, the crosses of C. annuum with C. baccatum or with C. pubescens were completely incompatible. The cause of incompatibility between C. annuum and C. baccatum was determined to be embryo and/or endosperm abortion. Several crosses determined as incompatible by the conventional crossability test were able to be re-determined as partial-compatible through an embryo rescue technique. Consequently, this experiment extended the boundary of breeding by interspecific hybridization in chili peppers.
Yoon, Jae Bok,Yang, Dong Cheol,Do, Jae Wahng,Park, Hyo Guen Japanese Society of Breeding 2006 Breeding science Vol.56 No.1
<P>Interspecific hybridization is essential to introgress resistance genes from <I>Capsicum baccatum</I>, a related species of cultivated pepper (<I>C. annuum</I>), since reliable genetic resources resistant to anthracnose have recently been identified within the <I>C. baccatum</I> germplasm. In conventional interspecific hybridization between the two species, hybrids could not be generated due to embryo abortion, which has been known to be a post-fertilization genetic barrier. Some partially compatible cross combinations, determined through observations of embryo development after pollination, were identified using a large number of accessions of <I>C. annuum</I> as pistillate parents. Embryo rescue technique was successfully employed to produce hybrids in these partially compatible crosses. Immature seeds bearing torpedo or early cotyledonary embryos, developed 35–40 days after pollination, were excised and the embryos were cultured on MS medium with sucrose and plant growth regulators. Hybridity was confirmed by observation of corolla yellow spot as a dominant species-specific trait of <I>C. baccatum</I> and using random amplified polymorphic DNA (RAPD) marker analysis. All the hybrid plants displayed vigorous growth but complete pollen sterility. The hybrid sterility was overcome through intensive backcrossing using <I>C. annuum</I> as the pollen parent. Consequently, hundreds of interspecific BC<SUB>1</SUB>F<SUB>1</SUB> progenies were raised, and introgression of anthracnose resistance was confirmed in this segregating population.</P>
Identification of QTLs for Resistance to Anthracnose to Two Colletotrichum Species in Pepper
이준대,Jae Wahng Do,윤재복,홍지화 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4
Pepper (Capsicum spp.) anthracnose caused by Colletotrichum spp. is a serious disease damaging pepper production in Asian monsoon regions. For QTL mapping analyses of anthracnose resistance, an introgression BC1F2 population was made by interspecific crosses between Capsicum annuum 'SP26' (susceptible recurrent parent) and Capsicum baccatum 'PBC81' (resistant donor). Both green and red fruits were inoculated with C. acutatum 'KSCa-1' and C. capsici 'ThSCc-1' isolates and the disease reactions were evaluated by disease incidence, true lesion diameter, and overall lesion diameter. On the whole, distribution of anthracnose resistance was skewed toward the resistant parent. It might indicate that one or two major QTLs are present. The introgression map consisting of 13 linkage groups with a total of 218 markers (197 AFLP and 21 SSR), covering a total length of 325 cM was constructed. Composite interval mapping analysis revealed four QTLs for resistance to 'KSCa-1' and three QTLs for 'ThSCc-1' isolate, respectively. Interestingly, the major QTLs (CaR12.2 and CcR9) for resistance to C. acutatum and C. capsici, respectively, were differently positioned but there were close links between the minor QTL CcR12.2 for C. capsici and major QTL CaR12.2 as well as the minor QTL CaR9 for C. acutatum and major QTL CcR9. These results will be helpful for marker-assisted selection and pyramiding two different anthracnose-resistant genes in commercial pepper breeding.