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엄영섭,유광선,박재용,한석영 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-
It is important to represent limit state function in reliability-based design optimization. Since it is very difficult to explicitly represent a limit state function in topology optimization, it is necessary to make the limit state function an approximate function. In case of implementing RSM, the limit state function can easily approximated. In this study, the limit state function of structures was approximated using ADAPRES to obtain the effective response surface modeling as reducing the experiment points. From the result of carrying out the BESO using the ADAPRES, it is concluded that the ADAPRES can be applied to RETO and is more effective than RSM in computing cost.
Eom, Su-Keun,Kong, Min-Woo,Kang, Myoung-Jin,Lee, Jae-Gil,Cha, Ho-Young,Seo, Kwang-Seok IEEE 2018 IEEE electron device letters Vol.39 No.11
<P>This letter reports high-quality plasma-assisted atomic-layer-deposited HfO<SUB><I>x</I></SUB>N<SUB><I>y</I></SUB> by using isopropyl alcohol (IPA) oxidant and cyclic N<SUB>2</SUB> plasma treatment demonstrated on n-type In<SUB>0.53</SUB>Ga<SUB>0.47</SUB>As. Improved interface characteristics, with suppressed frequency dispersion and surface oxidation, were demonstrated and resulted in significantly decreased interface trap density ( <TEX>${D} _{\text {it}}$</TEX>) of <TEX>${4.5}\times {10}^{{11}}$</TEX> eV<SUP>−1</SUP>cm<SUP>−2</SUP> at the mid-gap level with outstanding inversion behaviors. In addition, to verify true inversion, transition frequency ( <TEX>$\omega _{\text{m}}$</TEX>) of 4 kHz was extracted. The improvement mechanism of the proposed technology is assumed to be that nitrogen plasma reduces oxygen vacancies that act as oxygen diffusion paths and with the use of IPA oxidant the interface would be strongly protected during pre- and post-dielectric deposition.</P>
Eom, Sung-Hwan,Lee, Dae-Sung,Jung, Yeoun-Joong,Park, Jae-Hong,Choi, Ji-Il,Yim, Mi-Jin,Jeon, Jeong-Min,Kim, Hyun-Woo,Son, Kwang-Tae,Je, Jae-Young,Lee, Myung-Suk,Kim, Young-Mog Springer-Verlag 2014 Applied microbiology and biotechnology Vol.98 No.23
<P>To find more effective ways of overcoming methicillin-resistant Staphylococcus aureus (MRSA), there has been considerable interest in the use of marine-derived constituents as alternatives to control pathogenic microorganisms. In this study, we investigated whether phlorofucofuroeckol-A (PFF) isolated from the edible brown alga Eisenia bicyclis suppressed production or function of penicillin-binding protein 2a (PBP2a). The antimicrobial mode of action of PFF in MRSA was identified by measuring cell membrane integrity and using the time-kill curve method. We attempted to determine the antimicrobial effects of PFF on the expression level of the resistance determinants mecA and its regulatory genes mecI and mecR1 in MRSA by reverse transcriptase polymerase chain reaction. PFF suppressed mecI, mecR1, and mecA gene expression in a dose-dependent manner. In addition, we revealed PFF mediates the suppressive effect of PBP2a expression in MRSA by Western blot analysis. PFF suppressed production of the PBP2a protein, suggesting that PFF probably acts by controlling the methicillin resistance-associated genes involved in the cell wall and production of PBP2a. These results demonstrate that PFF isolated from E. bicyclis significantly suppressed the expression of the methicillin resistance-associated genes and production of PBP2a, which is considered the primary cause of methicillin resistance.</P>
Eom, Gyeong Tae,Song, Jae Kwang,Ahn, Jung Hoon,Seo, Yeon Soo,Rhee, Joon Shick American Society for Microbiology 2005 Applied and environmental microbiology Vol.71 No.7
<B>ABSTRACT</B><P>The ABC transporter (TliDEF) from <I>Pseudomonas fluorescens</I> SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in <I>Escherichia coli</I>, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficiency were identified by coexpressing the mutated <I>tliD</I> library with the wild-type <I>tliA</I> lipase in <I>E. coli</I> and by screening the library with a tributyrin-emulsified indicator plate assay and a microtiter plate-based assay. Four selected mutants from one round of error-prone PCR mutagenesis, T6, T8, T24, and T35, showed 3.2-, 2.6-, 2.9-, and 3.0-fold increases in the level of secretion of TliA lipase, respectively, but had almost the same level of expression of TliD in the membrane as the strain with the wild-type TliDEF transporter. These results indicated that the improved secretion of TliA lipase was mediated by the transporter mutations. Each mutant had a single amino acid change in the predicted cytoplasmic regions in the membrane domain of TliD, implying that the corresponding region of TliD was important for the improved and successful secretion of the target protein. We therefore concluded that the efficiency of secretion of a heterologous protein in <I>E. coli</I> can be enhanced by in vitro engineering of the ABC transporter.</P>