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RISS 인기검색어
- 검색키워드
- 저자 : Huang Wenshu (검색결과 3 건)
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- 유료
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Yujia Bai,Wenshu Huang,Yongxia Tao,Zuoshan Feng 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1
The precise mechanism by which asafoetida extractinfluenced the growth of Pleurotus ferulae mycelium wasinvestigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group,the two asafoetida extracts played a significant role in promotingthe growth of P. ferulae. Two-dimensional electrophoresis andLC-MS/MS were performed to examine changes in the expressionof proteins in the mycelium of P. ferulae. A total of eightdifferentially expressed proteins were identified, including a heatshock protein, a flavin-containing monooxygenase, and a NADPH:quinone oxidoreductase type IV, as well as hypothetical andunknown proteins. Expression change of these proteins confirmedthat asafoetida extracts significantly affected P. ferulae growth,metabolism, and secondary product metabolism. These resultsprovide new insights into biochemical mechanisms underlying therelationship between P. ferulae and asafoetida.
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Bai, Yujia,Huang, Wenshu,Tao, Yongxia,Feng, Zuoshan The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1
The precise mechanism by which asafoetida extract influenced the growth of Pleurotus ferulae mycelium was investigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group, the two asafoetida extracts played a significant role in promoting the growth of P. ferulae. Two-dimensional electrophoresis and LC-MS/MS were performed to examine changes in the expression of proteins in the mycelium of P. ferulae. A total of eight differentially expressed proteins were identified, including a heat shock protein, a flavin-containing monooxygenase, and a NADPH: quinone oxidoreductase type IV, as well as hypothetical and unknown proteins. Expression change of these proteins confirmed that asafoetida extracts significantly affected P. ferulae growth, metabolism, and secondary product metabolism. These results provide new insights into biochemical mechanisms underlying the relationship between P. ferulae and asafoetida.
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Proteomic analyses on the browning of shade-dried Thompson seedless grape
Liu Fengjuan,Huang Wenshu,Feng Zuoshan,Tao Yongxia,Fan Yingying,He Weizhong,Li XiaoLi,Fang Xiaotong,Wang Cheng,Bai Yujia 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.3
China is one of the main producers in the worldwide raisin market. Most China’s raisins are produced in Xinjiang where the Thompson seedless grape ( Vitis vinifera L.cv.Thompson seedless) is the main variety of green raisin. However, the browning of Thompson seedless grape during drying has been well-acknowledged as the primary factor affecting the development of the raisin industry. Data independent acquisition (DIA)-based protein profiling was performed on fresh and shade-dried Thompson seedless grapes. As a result, 5431 proteins were identified, among which the amounts of 739 proteins in fresh grape were found to be significantly different with those in dried grape. The functional annotation based on the Blast2GO showed that the ‘organic substance metabolic process’, ‘regulation of molecular function’, ‘enzyme regulator activity’, and ‘isomerase activity’ related proteins became very active during browning. Further analyses revealed that the browning-related proteins, which with significant different amounts in fresh and in dried grapes, are primarily involved in the phenylpropanoid biosynthesis, tyrosine metabolism, phenylalanine metabolism, oxidative phosphorylation metabolism, plutathione metabolism, peroxisome pathway, and fatty acid degradation. And five random differential proteins were verified with parallel reaction monitoring (PRM). The PRM results were in agreement with the DIA data. The main browning-related proteins of Thompson seedless grape were identified in this study. Their properties were tested, and their roles in the browning mechanism were indicated. This will lay base to a better understanding on the enzymatic browning of Thompson seedless grape, and it will also provide guidance for controlling the quality of Thompson seedless grapes in industry.
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