Development of Hepatitis Vaccine in Genetically Engineered Mammalian Cell

Himadri, Samanta,Choi, Kyung-Hee 中央大學校 遺傳工學硏究所 1988 遺傳工學硏究論集 Vol.1 No.1

B형 간염 바이러스 표면항원 유전자가 포유동물세포에서 발현되도록 bovine papilloma virus(BPV)를 vector로 사용하여 몇가지 재조합 플라스미드를 제조하였다. promoter는 mouse metallotheionin promoter를 사용하였고, BPV의 크기를 변화시켰으며 또한 HBsAg 유전자의 polyadenylation signal을 제거하기도 하였다. 포유동물에서 세가지의 재조합 플라스미드는 모두 HBsAg을 발현시켰으며, BPV유전자와 HBV유전자가 완전히 들어있는 vector에 의해 가장 많은 HBsAg이 생산되었다. HBV DNA는 episomal 상태로 남아있는것이 아니고, 마우스세포의 염색체에 삽입되어 있는것이 확인되었으며, HBV DNA의 copy 수와 HBsAg의 생산량과는 비례하지 않는 것이 밝혀졌다. HBsAg 생산세포는 2-3개월동안 계대배양의 필요없이 계속해서 HBsAg을 배양액에 분비하였다. 이를 정제하여 E.M.으로 관찰한 결과 particle을 형성함이 확인되었고, western blot 분석을 통해 pre-s를 포함하고 있음을 밝혔다. We have expressed the surface antigen of hepatitis B virus (HBV) in mouse fibroblast cell line using metallothionein-bovine papilloma virus vectors. We have used three different recombinant plasmids and find that the expression level varies significantly from one plasmid to the other. One of the constructs gave very high express level and has been characterized further. We have failed to detect any plasmid in the episomal form in the producing clones and all the HBV DNA found to be integrated in the mouse chromosome at more than one site. We do not find any linear relationship between the number of copies HBV DNA and the level of the surface antigen produced. Cells can continuously be grown in the presence of cadmium chloride without splitting for more than two months and the secreted surface antigen be harvested during this period. The antigen is secreted as particle as have been found in electron micrograph of purified antigen. From western blot studies we find that the antigen contains the pre-s region.

Direct numerical simulation of evaporation in a biporous media

Himadri Chattopadhyay,Sudip K. Samanta,Gautam Biswas,Bharat B. Sharma 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

Biporous media having clusters of size range 300 - 425 µm, 425 - 600 µm, > 600 µm have been sintered at 950 °C , 180 min using gas atomized spherical particles (D50 of 90 microns) of pure copper in reducing atmosphere of pure hydrogen gas. MicroCT scan of biporous samples have been performed to and then images have been reconstructed to 3D CAD models, which further have been discretised into tetrahedral mesh using image processing and reconstruction software Avizo Fire 8.0. Direct numerical simulations have been performed to obtain Evaporative heat transfer coefficient (EHTC) over the surface of these biporous clusters. The transient evaporation phenomena and effect of cluster size on evaporation has been investigated in this paper. These results have been compared with the experimental results available in literature for evaporative heat transfer coefficient for biporous media.

Influence of Nanodispersed Organoclay on Rheological and Swelling Properties of Ethylene Propylene Diene Terpolymer

Himadri Acharya,Suneel K. Srivastava 한국고분자학회 2006 Macromolecular Research Vol.14 No.2

Block Copolymer Micelles with Near Infrared Metal Phthalocyanine Dyes for Laser Induced Writing

Acharya, Himadri,Yoon, Bokyung,Park, Youn Jung,Bae, Insung,Park, Cheolmin WILEY-VCH Verlag 2010 Macromolecular Rapid Communications Vol.31 No.12

<P>A route has been developed to disperse metal-containing phthalocyanine dyes in a non-polar medium based on amphiphilic block copolymer micelles of poly[styrene-block-(4-vinylpyridine)] (PS-b-P4VP) and poly[styrene-block-(acrylic acid)] (PS-b-PAA) copolymers. Polar P4VP and PAA efficiently encapsulate cobalt(II), manganese(II), and nickel(II) phthalocyanine dyes by axial coordination of nitrogen and µ-oxo bridged dimerization with the transition metals, respectively. Good dispersion of the dyes is confirmed by the linear enhancement of Q-bands in UV–vis absorption spectra with dye concentration. A thin monolayered PS-b-P4VP micelle film that contained a nickel(II) phthalocyanine dye which efficiently adsorbs a laser beam on a localized area to generate a local heat higher than the glass transition temperatures of both blocks. One-dimensional laser writing on the dye-containing film allows the fabrication of a few submicrometer wide line patterns in which the self-assembled nanostructure of the block copolymer is modified by the directional heat arising from laser scanning.</P><P> <img src='wiley_img_2010/10221336-2010-31-12-MARC201000032-gra001.gif' alt='wiley_img_2010/10221336-2010-31-12-MARC201000032-gra001'> </P> <B>Graphic Abstract</B> <P>Metal-containing (Co<SUP>II</SUP>, Mn<SUP>II</SUP>, and Ni<SUP>II</SUP>) phthalocyanine (Pc) dyes dispersed in a non-polar medium based on amphiphilic block copolymer micelles of poly[styrene-block-(4-vinylpyridine)] (PS-b-P4VP) and poly[styrene-block-(acrylic acid)] (PS-b-PAA) copolymers are investigated. Linear enhancement of Q-bands in the UV–vis absorption spectra with dye concentration confirms the good dispersion. Laser absorption by a thin monolayered PS-b-P4VP micelle film with Ni-Pc allowed the writing of line patterns. <img src='wiley_img_2010/10221336-2010-31-12-MARC201000032-content.gif' alt='wiley_img_2010/10221336-2010-31-12-MARC201000032-content'> </P>

Influence of Nanodispersed Organoclay on Rheological and Swelling Properties of Ethylene Propylene Diene Terpolymer

Acharya Himadri,Srivastava Suneel K. The Polymer Society of Korea 2006 Macromolecular Research Vol.14 No.2

The dispersion of organoclay in ethylene propylene diene terpolymer (EPDM) matrix was correlated with the rheological and swelling properties of nanocomposites. X-ray diffraction pattern (XRD) and transmission electron microscopic (TEM) analysis exhibited the disordered-intercalated structure of EPDM/organoclay nanocomposite. The extent of the disordered phase increased with increasing organoclay content up to a limiting value of 3 wt% after which equilibrium tended towards intercalation. The dispersion effect of organoclay in EPDM matrix was clarified by the physicochemical properties like rheological response and swelling thermodynamics in toluene. The increase in viscoelastic properties of EPDM nanocomposite with increasing organoclay content up to 3 wt%, followed by a subsequent decrease up to 4 wt%, was correlated in terms of the disordered and ordered states of the dispersed nano-clay sheets. Swelling measurements revealed that the change in entropy of the swelling increased with the increase in disorder level but decreased with the increase in intercalation level of organoclay in the disordered-intercalated nanocomposite. The increase in solvent uptake was comparable with the free volume in EPDM matrix upon inclusion of silicate particles, whereas the inhibition in solvent uptake for higher organoclay loading was described by bridging flocculation.

마우스세포를 이용한 B 형 간염 바이러스의 표면항원 유전자의 발현

최경희,박완제 ( Himadri Samanta ) 한국유전학회 1988 Genes & Genomics Vol.10 No.4

N/A

Controlled Dewetting of Thin Self Assembled Polymer Films in Multiple Length Scales

박철민,김태희,Himadri Archaya,이근탁 한국고분자학회 2009 한국고분자학회 학술대회 연구논문 초록집 Vol.2009 No.4

Modulation of coupled surface plasmon band of position selective Ag and Au nanoparticles in block copolymer films by single-walled carbon nanotube

V.D.Babu,Himadri acharya,김태희,박철민 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.2

Effect of Mn Addition on the Mechanical Properties of Al–12.6Si Alloy: Role of Al15(MnFe)3Si2 Intermetallic and Microstructure Modification

Prosanta Biswas,Surajit Patra,Himadri Roy,Chandra Sekhar Tiwary,Manas Paliwal,Manas Kumar Mondal 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.6

Effect of manganese (Mn) addition (0.0, 1.0, 2.0 and 3 wt%) on the microstructural morphology, hardness, tensile propertiesand fracture behaviour of the gravity cast eutectic Al–12.6Si alloy has been studied through XRD analysis, chemical analysis,optical metallography, FESEM analysis, energy dispersive spectroscopy analysis, hardness test, tensile test and quantitativephase analysis. As-cast Al–12.6Si–0.0Mn alloy has a non-uniformly distributed coarser and irregular shape primary andeutectic silicon particles inside the α-Al phase, and both the Si phase have very sharp corners. Whereas, the 1 wt% Mn addedalloy has uniformly distributed fine eutectic and primary Si particles with blunt corners. Further, the addition of 1.0 wt% Mnforms very few (0.26 vol %) irregular shape Al15(MnFe)3Si2 intermetallic phase within the α-Al phase and eutectic Si phase. But, 2.0 wt% and 3 wt% Mn added alloy has an irregular shape coarse plate-like Al15(MnFe)3Si2 intermetallic phase besidesthe primary and eutectic Si phase. The bulk hardness of the Al–12.6Si alloy is increased with an increase in Mn concentrationas the harder Al15(MnFe)3Si2 intermetallic phase forms and both the Si phase morphology modify. The microhardness ofthe constituent phases also varies with the change in Mn concentration in the alloy. The Mn addition improved the ultimatetensile strength, yield strength, and elongation (%) of the alloy. However, fractographs reveal that the brittle mode of fracturehas been increased due to the presence of a higher volume of brittle Al15(MnFe)3Si2 intermetallic in 2.0 and 3.0% Mn alloy. On the other hand, the amount of brittle and cleavage fracture of Si particles decreased, and ductile fracture with dimplesformation increased in 1.0 wt%Mn added alloy.

Electrochemical Advanced Oxidation of Lamotrigine at Ti/DSA (Ta₂O₅-Ir₂O₅) and Stainless Steel Anodes

Meena, Vinod Kumar,Ghatak, Himadri Roy The Korean Electrochemical Society 2022 Journal of electrochemical science and technology Vol.13 No.2

The study presents kinetics of degradation and mineralization of an anti-epileptic drug Lamotrigine (LAM) in the aqueous matrix by electrochemical advanced oxidation process (EAOP) on Ti/DSA (Ta₂O₅-Ir₂O₅) and Stainless Steel (SS) anodes using sodium sulphate as supporting electrolyte. On both the anodes, kinetic behaviour was pseudo-first-order for degradation as well as mineralization of LAM. On Ti/DSA anode, maximum LAM degradation of 75.42% was observed at an associated specific charge of 3.1 (Ah/litre) at a current density of 1.38 mA/cm² and 100 ppm Na₂SO₄ concentration. Maximum mineralization attained was 44.83% at an associated specific charge of 3.1 (Ah/litre) at a current density of 1.38 mA/cm² and 50 ppm concentration of Na₂SO₄ with energy consumption of 2942.71 kWh/kgTOC. Under identical conditions on SS anode, a maximum of 98.92% LAM degradation was marked after a specific charge (Q) of 3.1 (Ah/litre) at a current density of 1.38 mA/cm² and 100 ppm concentration of Na₂SO₄. Maximum LAM mineralization on SS anode was 98.53%, marked at a specific charge of 3.1 (Ah/litre) at a current density of 1.38 mA/cm² and 75 ppm concentration of Na₂SO₄, with energy consumption of 1312.17 kWh/kgTOC. Higher Mineralization Current Efficiency (MCE) values were attained for EAOP on SS anode for both degradation and mineralization due to occurrence of combined electro-oxidation and electro-coagulation process in comparison to EAOP on Ti/DSA anode due to occurrence of lone electro-oxidation process.