악성 흉막삼출액에서 Bleomycin을 이용한 흉막유착설의 시술효과

박정현,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.1

목적: 악성 흉막삼출액이 반복적이고 지속적으로 발생하는 경우, 호흡곤란, 흉통등의 증상을 유발시키므로 흉막경화제에 의한 흉막유착술을 시행할 수 있다. 흔히 사용되던 talc의 심각한 부작용이 보고되고 있고, doxycycline 주사제도 국내에서 발매되지 않고 있다. 이에 연자들은 항암제로 소개된 bleomycin을 이용한 흉막유착술의 단기와 중기효과, 부작용 및 생존율 등을 전향적으로 확인하였다. 방법: 2000년 1월부터 2001년 12열까지 원광대학교 부속병원에 내원하여 반복적이고 지속적인 악성 흉막삼출액으로 흉막유착술이 필요한 26명의 환자를 대상으로 특별한 전 처치 없이 bleomycin 60 unit를 식염수와 함께 흉강 내 주입 후, 1일 후의 흉관에 의한 배액량을 확인하고 부작용을 관찰하였으며, 1개월 후에 흉부 방사선상으로 재 저류 유무를 확인하였다. 또한 시술 반응군과 비반응군으로 나누어 중앙생존기간을 비교분석 하였다. 결과: 시술 1일 후 26예중 10예(38%)에서 배액이 없었고, 8예(31%)에서 배액량이 100ml이하였으며, 8예(31%)는 100ml이상이었으나 시술 전보다 감소하였으며 증가된 경우는 없었다. 유의할만한 부작용은 4예(고열 3예, 흉통 1예)에서 발생하였으나, 대증요업으로 조절되었다. 시술 1개월 후 흉부 방사선과 26예중 9예(35%)는 재 저류액이 없었고, 10예(38%)는 저류액이 있으나 시술 전보다 감소하였으며, 1예(4%)는 이전과 변화가 없었고, 6예(23%)는 시술 전보다 저류액이 증가하였다. 시술 후 단기 및 중기효과를 반응군과 비반응군으로 나누어 중앙생존기간으로 양군간을 비교하였을 때, 반응군에서 연장이 되었으나 통계적으로 유의한 차이는 없었다. 결론: 이상의 결과로서 bleomycin에 의한 흉막유착술은 다른 경화제보다 특별한 전 처치가 필요 없는 간편하고, 비교적 부작용이 적은 안전한 방법이며, 효과적인 치료성적을 보이는 시술로 사료된다. Background : Malignant pleural effusions are common and significant problems in patients with advanced malignancy. Repeated thoracentesis provides temporary symptomatic improvement, but most patients progressively cause dyspnea or pleuritic pain. So pleurodesis with sclerosing agent is effective as a palliative treatment. There are many drugs used as pleural sclerosing agents. But doxycycline hasn't been used in Korea since 1999. And recently talc has been reported serious side effects. In this study, pleurodesis with bleomycin was prospectively investigated for its short term and middle period effects and its side effects. Methods : Twenty-six patients with malignant pleural effusion were prospectively analyzed to estimate the effects of bleomycin pleurodesis. Without special management before the procedure, 60 units of bleomycin in 50-100ml of normal saline were instilled into the pleural space via a small bore catheter(8-10 F). Andthen repeated positional changes and rotations were done for 2 hours. We measured the drainage amount and evaluated the side effects after 24 hours and checked the existence of the effusion by chest radiography after 1 month. Results : After 24 hours, the drainage amount showed that the responder group(none or ≤ 100ml) was 69%, and that the nonresponder group(> 100ml) was 31%. Side effects (high fever in 3 cases and severe pleuritic pain in 1 case) were 15%(4/26), but patients recovered easily by symptomatic management. After 1 month, chest radiography showed that the response rate(complete or partial response) was 73%. Differences of survival times between responder and nonresponder group was not statistically significant. Conclusions : We are suggest that bleomycin as a pleural sclerosing agent is a relatively simple, safe and effective agent. But a prospective study with a larger number of patients must be warranted.

폐암으로 오인된 이물질에 의한 기관지 육아종증 1예

정종훈,김소영,김휘정,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

한국산 가시톡토기과의 분류학적 검토

이병훈,이원구,김학렬 全北大學校 生物學硏究所 1984 生物學硏究年報 Vol.5 No.-

A preliminary test concerning variabilities of some Tomoceridae species of collembola has been made using scanning electron microscopy and enzyme analysis. No definite taxono mical characters were detected in the integument of 6 species belonging 2 subgenera of Tomocerus genus. The zymograms obtained by electrophoresis, however, revealed that th subgeneric characters at times should be questioned for its monophyletic meaning as suggested by those of Tomocerus (Tomocerus) kinoshitai showing a particular pattern and Tomocerus (Tomocerina) liliputanus for its apprectable variability. The distinctive zymograms of T.laxalamella might also justify the creation of a new subgenus when considered in combination with the morphological characteristics of mucron. Neither population nor interspecific difference were observed, however, in and between T. cuspidatus and T. ocreatus, which would remain problematic and necessitate further works to confirm. Tomocerus laxalamella and T. liliputanus showed more of less variabilites whereas Homidia koreana of Entomobryidae, used as out-group for comparison, displayed a prominent individual variations in a few loci.

자발성 기흉을 동반한 폐림프관평활근종증 1예

안용환,정종훈,김소영,김휘정,김학렬,양세훈,최순호,문형배,조향정,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

Programmed death ligand-1 expression and its prognostic role in esophageal squamous cell carcinoma

Kim, Ryul,Keam, Bhumsuk,Kwon, Dohee,Ock, Chan-Young,Kim, Miso,Kim, Tae Min,Kim, Hak Jae,Jeon, Yoon Kyung,Park, In Kyu,Kang, Chang Hyun,Kim, Dong-Wan,Kim, Young Tae,Heo, Dae Seog Baishideng Publishing Group Inc 2016 WORLD JOURNAL OF GASTROENTEROLOGY Vol.22 No.37

<P><B>AIM</B></P><P>To investigate the expression and prognostic role of programmed death ligand-1 (PD-L1) in locally advanced esophageal squamous cell carcinoma (ESCC).</P><P><B>METHODS</B></P><P>A total of 200 patients with ESCC who underwent radical esophagectomy with standard lymphadenectomy as the initial definitive treatment in Seoul National University Hospital from December 2000 to April 2013 were eligible for this analysis. Tissue microarrays were constructed by collecting tissue cores from surgical specimens, and immunostained with antibodies directed against PD-L1, p16, and c-Met. Medical records were reviewed retrospectively to assess clinical outcomes. Patients were divided into two groups by PD-L1 status, and significant differences in clinicopathologic characteristics between the two groups were assessed.</P><P><B>RESULTS</B></P><P>Tumor tissues from 67 ESCC patients (33.5%) were PD-L1-positive. Positive p16 expression was observed in 21 specimens (10.5%). The H-score for c-Met expression was ≥ 50 in 42 specimens (21.0%). Although PD-L1-positivity was not significantly correlated with any clinical characteristics including age, sex, smoking/alcoholic history, stage, or differentiation, H-scores for c-Met expression were significantly associated with PD-L1-positivity (OR = 2.34, 95%CI: 1.16-4.72, <I>P</I> = 0.017). PD-L1 expression was not significantly associated with a change in overall survival (<I>P</I> = 0.656). In contrast, the locoregional relapse rate tended to increase (<I>P</I> = 0.134), and the distant metastasis rate was significantly increased (HR = 1.72, 95%CI: 1.01-2.79, <I>P</I> = 0.028) in patients with PD-L1-positive ESCC compared to those with PD-L1-negative ESCC.</P><P><B>CONCLUSION</B></P><P>PD-L1 expression is positively correlated with c-Met expression in ESCC. PD-L1 may play a critical role in distant failure and progression of ESCC.</P>

누에(Bombyx mori)의 卵形成過程에 따른 Vitellogenin의 分布 및 量的 變化

Kim, Hak-Ryul,Seo, Eul-Won The Korean Society for Integrative Biology 1983 동물학회지 Vol.26 No.2

누에의 卵形成時期동안 자성 혈림프에서 DEAE-cellullose column을 使用하여 vitellogenin을 순수하게 分離해 낸 후, 電氣泳動法과 免疫學的 方法을 使用하여 각 器官에 따른 vitellogenin의 分布와 이의 量的 變化를 調査하였다. 누에의 vitellogenin은 난성숙시기에 걸쳐 혈림프, 脂肪體 및 卵巢에 모두 分布하고 있으며, 특히 포화후 7일까지만 확인되었으며 그 이후 급격한 量的 減少를 나타내었으며, 또한 血蛋白質은 卵巢蛋白質과 最小한 3개의 同質인 蛋白質을 갖고 있는 것으로 보아 卵形成에 vitellogenin 이외의 다른 血蛋白質도 관여 되는 것 같다. Vitellogenin was purified from the haemolymph of female pupae during egg formation of Bombyx mori using DEAE-cellulose column and its quantitative change in various organs with age ws examined by electrophoresis and immunodiffusion. Vitellogenin is distributed in the haemolymph, fat body, and over the egg maturation and especially maintainsa constant level in the haemolymph until just before emergence, indicating that vitellogenin in released into the haemolymph at the same amount as it is taken up by oocytes during pupal period. Immunologically vitellogenin was confirmed to be in the fat body until 7 days after pupation and to undergo a drastic decline thereafter. Also, the interaction of anti-ovary proteins with haemolymph proteins showed at least 3 homogeneous proteins, indicating that other proteins as well as vitellogenin are involved in egg formation.

Bedaquiline and delamanid for the treatment of multidrug-resistant tuberculosis: a multicentre cohort study in Korea

Kim, Cheon Tae,Kim, Tae-Ok,Shin, Hong-Joon,Ko, Young Chun,Hun ,Choe, Yeong,Kim, Hak-Ryul,Kwon, Yong-Soo ERS Journals Ltd 2018 The European respiratory journal Vol.51 No.3

<P>Bedaquiline and delamanid were effective and safe for treating MDR-TB, with initial evidence of sequential administration of these two drugs as a viable treatment strategy for patients when an adequate treatment regimen cannot be constructed.</P>

Purification and Characterization of Hemolymph Origin Silk Gland Proteins of Wax moth, Galleria mellonella

Kim, Kyung-Bun,Kim, Hee-Ja,Lee, Chang-Seok,Lee, Sang-Yong,Kim, Hak-Ryul 한국곤충학회 2001 Entomological Research Vol.31 No.1

Hemolymph origin silk gland protein (HOSGP) was identified from the silk gland of wax moth, Galleria mellonella and characterized using antibodies against larval hemolymph protein and silk gland. Hemolymph and silk gland extracts were electrophoresed and western-blotted with each developmental stage. HOSGPs were present in the silk gland. To confirm the presence of HOSGP in other tissues, western-blotting was performed with extracts of Malpighian tubule, midgut and fat body. HOSGP was also present in the fat body. HOSGP was pre-sent in large amounts in the silk gland of the last instar larvae and wandering stages when silk is actively produced. HOSGP was partially purified by KBr density gradient ultracentrifugation and prep-cell, and characterized.

Regulation of NAD⁺- Specific Isocitrate Dehydrogenase from Pythium ultimum

Kim, Hak-Ryul,Weete, John D. Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.4

The $NAD^+$-specific activity of a dual coenzyme-specific isocitrate dehydrogenase (IDH; EC 1.1.1.41) from the primitive fungus Pythium ultimum was investigated to elucidate the regulatory factors that may influence the intracellular distribution of carbon and the availability of intermediates, e.g. citrate, for fatty acid synthesis. Inhibition of $NAD^+$-IDH activity by diphospho- and triphosphonucleotides (ATP, ADP, and GTP) reflected the sensitivity of this enzyme to cellular energy charge even though monophosphonucleotides (AMP and GMP) had little effect on activity. NADPH, but not NADH, substantially inhibited $NAD^+$-IDH activity, showing noncompetitive inhibition with isocitrate. Oxalacetate and ${\alpha}$-ketoglutarate showed competitive inhibition with isocitrate, while citrate and cis-aconitate showed mixed-noncompetitive inhibition with isocitrate. Inhibition by these substances ranged from 29 to 46% at 10 mM. The inhibitory effect of oxalacetate was increased synergistically by glyoxylate, which alone caused 31% uncompetitive inhibition at 10 mM, and a mixture of the two substances at 1 mM each showed 98% inhibition of $NAD^+$-IDH activity. The regulation of $NAD^+$-IDH in Pythium ultimum seems to be a complex process involving mitochondrial metabolites. The addition of glyoxylate (3 mM) and oxalacetate (3 mM) to the culture medium resulted in the production of 49% more lipid by P. ultimum.

Changes of RNA and Protein Content in Epidermis during Metamorphosis of Bombyx mori L.

Kim, Hak-Ryul,Yu, Jeong-Heui 한국곤충학회 1982 Korean journal of entomology Vol.12 No.1

누에 (Bombyx mori L.)의 변태기동안 표피의 RNA와 단백질의 농도 및 합성능을 Spectrophotometer(UV-360)와 Liquid Scintillation Counter (Beckman LS 100-C)로 측정 비교하였다. 표피의 RNA 농도는 전용초(고치형성기)에 최대에 달하였다가 그이후 감소하였으며 용화직후 다시 증가하였다. 또한 단백질 농도는 종령 유충말에 증가하였다가 그이후 감소하였으며 전용초에 최대에 달하였다가 다시 감소하였다. 혈림프내에 주입시킨 표피의 $^3$H-uridine의 방사능은 종령 유충말에서 전용말로 갈수록 감소하다가 다시 증가하여 용화후 1일에 최대에 달하였다. 또한 $^{14}$ C-leucine의 방사능은 종령 유충말과 전용말에 단백질농도의 증가양상과 일치한다. Concentration and synthetic activity of RNA and protein in epidermis during metamorphosis of Bombyx mori L. were determined by Spectrophotometer (UV-360) ana Liquid Scintillation Counter(Bookman LS 100-C). RNA concentration in epidermis reaches the peak at early prepupal stage (cocoon formation period) and falls down thereafter and then increase again just after pupation. Also, protein concentration rises at late last instar stage and decreases thereafter and then reaches the peak at late prepural stage folio wed by decrease. The radioactivity in epidermis of $\^$3/H-uriaine injected into haemocoel decreases during the transformation from late last instar larva to early prepupa and again increases and then reaches the peak 1 day after pupation. Also, radioactivity of $\^$14/C-leucine coincides with increasing pattern of protein at late instar larval and late prepupal stages.