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A dual-site modulated FRET-based two-photon ratiometric fluorescent probe for tracking lysosomal pH changes in living cells, tissues and zebrafish

Zhao, Xiongjie,Wang, Chao,Yuan, Gangqiang,Ding, Haiyuan,Zhou, Liyi,Liu, Xiaogang,Lin, Qinlu Elsevier 2019 Sensors and actuators. B Chemical Vol.290 No.-
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Abstract Lysosomal pH plays an essential role in mediating various biological processes such as immunization, cell metabolism and enzyme activity. Herein, by utilizing the fluorescence resonance energy transfer (FRET) strategy, a two-photon (TP) ratiometric fluorescent probe (NpLys-pH) has been developed for tracking of lysosomal pH changes in living cells, tissues, and zebrafish. NpLys-pH was constructed by conjugating a pH turn-on TP fluorophore 1 (D-π-A-structured naphthalene derivative) with a pH turn-off naphthalimide fluorophore 2 via a non-conjugated linker. Meanwhile, NpLys-pH has two potential pH response sites that modulate the fluorescence signal by ICT and PET, respectively. The FRET process exists between the TP fluorophore 1 and naphthalimide fluorophore 2. In addition, NpLys-pH respond to pH rapidly and reversibly with high selectivity and sensitivity and has been applied for tracking lysosomal pH changes in living cells, tissues and zebrafish. We expect that the new probe present here can prompt the development of a wide variety of TP ratiometric fluorescent probes which can find application in detecting other important analytes in biological systems. Highlights <UL> <LI> A FRET-based TP ratiometric probe for tracking of lysosome pH changes. </LI> <LI> It has a pH turn-on donor and a pH turn-off acceptor. </LI> <LI> NpLys-pH possesses two well-resolved emission peaks separated by 115 nm. </LI> <LI> It successfully tracked lysosome pH changes in live cells, tissues, and zebrafish. </LI> </UL>
2
Nephroprotective effect of astaxanthin against trivalent inorganic arsenic-induced renal injury in wistar rats

Xiaona Wang,Haiyuan Zhao,Yilan Shao,Pei Wang,Yanru Wei,Weiqian Zhang,Jing Jiang,Yan Chen,Zhigang Zhang 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.1
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 ScienceON
 
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 DBpia
 
 DBpia
Inorganic arsenic (iAs) is a toxic metalloid found ubiquitously in the environment. In humans, exposure to iAs can result in toxicity and cause toxicological manifestations. Arsenic trioxide (As2O3) has been used in the treatment for acute promyelocytic leukemia. The kidney is the critical target organ of trivalent inorganic As (iAsⅢ) toxicity. We examine if oral administration of astaxanthin (AST) has protective effects on nephrotoxicity and oxidative stress induced by As2O3 exposure (via intraperitoneal injection) in rats. Markers of renal function, histopathological changes, Na+-K+ ATPase, sulfydryl, oxidative stress, and As accumulation in kidneys were evaluated as indicators of As2O3 exposure. AST showed a significant protective effect against As2O3-induced nephrotoxicity. These results suggest that the mechanisms of action, by which AST reduces nephrotoxicity, may include antioxidant protection against oxidative injury and reduction of As accumulation. These findings might be of therapeutic benefit in humans or animals suffering from exposure to iAsⅢ from natural sources or cancer therapy.