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        Innate immunity and carbohydrate metabolism alterations precede occurrence of subclinical mastitis in transition dairy cows

        Dervishi, Elda,Zhang, Guanshi,Hailemariam, Dagnachew,Dunn, Suzana M.,Ametaj, Burim N. Korean Society of Animal Sciences and Technology 2015 한국축산학회지 Vol.57 No.12

        Background: This study examined whether activation of innate immunity and alterations of carbohydrate and lipid metabolism precede development of subclinical mastitis (SCM). Methods: Blood samples were collected from the coccygeal vein from 100 Holstein dairy cows at -8, -4, disease diagnosis week, and +4 weeks postpartum. Six healthy cows (controls - CON) and six cows that showed clinical signs of SCM were selected for serum analyses. All serum samples were analyzed for acute phase proteins (APP) haptoglobin (Hp) and serum amyloid A (SAA); proinflammatory cytokines including interleukin 1 (IL-1), IL-6, and tumor necrosis factor (TNF) and serum lactate, BHBA, and NEFA concentration. Data of DMI, milk production, and milk composition were recorded and analyzed. Results: The results showed that cows with SCM had greater concentrations of SAA, TNF (P < 0.01), and lactate before expected day of parturition (P < 0.05) compared to CON cows. Cows with SCM showed greater concentrations of lactate starting at -8 weeks (P < 0.05) and TNF starting at -4 weeks prior to the expected day of parturition (P < 0.01). Interestingly, at -4 weeks, concentrations of IL-1 and Hp were lower in cows with SCM compared to healthy cows (P < 0.01) followed by an increase during the week of disease diagnosis (P < 0.05). Subclinical mastitis was associated with lower DMI, at -4 weeks before calving, milk production (P < 0.05) and increased somatic cell counts (SCC) (P < 0.01). Conclusions: Results of this study suggest that SCM is preceded by activated innate immunity and altered carbohydrate metabolism in transition dairy cows. Moreover the results support the idea that Hp, lactate, and SAA, at -8 weeks, and TNF and IL-1 at -4 weeks can be used as early indicators to screen cows during dry off for disease state.

      • KCI우수등재

        RESEARCH : Open Access ; Innate immunity and carbohydrate metabolism alterations precede occurrence of subclinical mastitis in transition dairy cows

        ( Elda Dervishi ),( Guanshi Zhang ),( Dagnachew Hailemariam ),( Suzana M. Dunn ),( Burim N. Ametaj ) 한국동물자원과학회(구 한국축산학회) 2015 한국축산학회지 Vol.57 No.46

        Background: This study examined whether activation of innate immunity and alterations of carbohydrate and lipid metabolism precede development of subclinical mastitis (SCM). Methods: Blood samples were collected from the coccygeal vein from 100 Holstein dairy cows at -8, -4, disease diagnosis week, and +4 weeks postpartum. Six healthy cows (controls . CON) and six cows that showed clinical signs of SCM were selected for serum analyses. All serum samples were analyzed for acute phase proteins (APP) haptoglobin (Hp) and serum amyloid A (SAA); proinflammatory cytokines including interleukin 1 (IL-1), IL-6, and tumor necrosis factor (TNF) and serum lactate, BHBA, and NEFA concentration. Data of DMI, milk production, and milk composition were recorded and analyzed. Results: The results showed that cows with SCM had greater concentrations of SAA, TNF (P < 0.01), and lactate before expected day of parturition (P < 0.05) compared to CON cows. Cows with SCM showed greater concentrations of lactate starting at -8 weeks (P < 0.05) and TNF starting at -4 weeks prior to the expected day of parturition (P < 0.01). Interestingly, at -4 weeks, concentrations of IL-1 and Hp were lower in cows with SCM compared to healthy cows (P < 0.01) followed by an increase during the week of disease diagnosis (P < 0.05). Subclinical mastitis was associated with lower DMI, at -4 weeks before calving, milk production (P < 0.05) and increased somatic cell counts (SCC) (P < 0.01). Conclusions: Results of this study suggest that SCM is preceded by activated innate immunity and altered carbohydrate metabolism in transition dairy cows. Moreover the results support the idea that Hp, lactate, and SAA, at -8 weeks, and TNF and IL-1 at -4 weeks can be used as early indicators to screen cows during dry off for disease state.

      • KCI등재

        Effect of Temperature and Revolution on the Microstructure, Texture and Microhardness of the AZ80 Alloy Cup Prepared by Rotating Backward Extrusion

        Xin Che,Qiang Wang,Xingwang Duan,Min Wang,Linfeng He,Miao Cao,Shuang Liu,Guanshi Zhang,Zhimin Zhang 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.11

        Microstructure evolution, phase development and mechanical property of AZ80 alloy cup with various processing temperatures(573 K, 613 K, 653 K) and rotating revolutions (N = 0, 5, 50, 100) during rotating backward extrusion (RBE)were explored. The study founded that reducing deformation temperature or increasing revolution could promote the grainrefinement and DRX proportion. And the sample deformed at the condition of 573 K and N = 100 exhibited the best grainrefinement effect (grain size was 2.5 μm, DRX ratio was up to 99.01%). The bulk eutectic phase existed at all deformationtemperatures, while the dynamic precipitation was only precipitated at temperature of 573 K and 613 K. Increasing thedeformation temperature or revolution could reduce the proportion of the second phase. And an increase in revolution or areduction of temperature would reduce the average size of second phase, due to the phase could be broken by large strainand resolved with elevated temperature. Moreover, increasing the revolution and deformation temperature could promotethe texture weakening, which would be contributed to the occurrence of new recrystallization grains with random orientationand the activation of non-basal slip system. The mechanical property of microhardness was significantly improved withrotating revolution increasing and deformation temperature decreasing. Supported by the grain refinement and dispersionstrengthening, the sample deformed at condition of 573 K and N = 100 achieved maximum hardness values of 96.3 HV.

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