Expression Level of Valosin Containing Protein is Associated with Prognosis of Primary Orbital MALT Lymphoma

Zhu, Wen-Wen,Kang, Li,Gao, Ya-Ping,Hei, Yan,Dong, Jie,Liu, Yu,Xiao, Li-Hua,Yang, Guang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Objective: To investigate whether the expression level of valosin-containing protein (VCP) is correlated with the prognosis of primary orbital mucosa-associated lymphoid tissue (MALT) lymphoma. Methods: VCP expression in 58 samples from primary orbital MALT lymphoma patients was determined by immunohistochemisty using monoclonal antibodies. Correlations between VCP expression level and prognosis were clarified by statistical analysis. Results: It was found that the percentage of VCP positive cells in samples of primary orbital MALT lymphoma ranged from 32% to 95%. The samples were divided into two groups (level 1 and level 2) according to the median value (45%) of the percentage of VCP positive cells. It was found that the expression level of VCP was significantly correlated with recurrence (P=0.003) and tumor size (P=0.008). At the same time, the 5-year disease-free and overall survival rate of patients of level 1 was significantly better than that of level 2 (P=0.001; P=0.032). There was no observed correlation between the expression level of VCP and other clinical features. Conclusion: VCP could be a useful marker for predicting the prognosis of primary orbital MALT lymphoma.

Systematic Review and Meta-Analysis of Antibiotic-Impregnated Shunt Catheters on Anti-Infective Effect of Hydrocephalus Shunt

Zhou, Wen-xiu,Hou, Wen-bo,Zhou, Chao,Yin, Yu-xia,Lu, Shou-tao,Liu, Guang,Fang, Yi,Li, Jian-wen,Wang, Yan,Liu, Ai-hua,Zhang, Hai-jun The Korean Neurosurgical Society 2021 Journal of Korean neurosurgical society Vol.64 No.2

Objective : Shunt infection is a common complication while treating hydrocephalus. The antibiotic-impregnated shunt catheter (AISC) was designed to reduce shunt infection rate. A meta-analysis was conducted to study the effectiveness of AISCs in reduction of shunt infection in terms of age, follow-up time and high-risk patient population. Methods : This study reviewed literature from three databases including PubMed, EMBASE, and Cochrane Library (from 2000 to March 2019). Clinical studies from controlled trials for shunt operation were included in this analysis. A subgroup analysis was performed based on the patient's age, follow-up time and high-risk population. The fixed effect in RevMan 5.3 software (Cochrane Collaboration) was used for this meta-analysis. Results : This study included 19 controlled clinical trials including 10105 operations. The analysis demonstrated that AISC could reduce the infection rate in shunt surgery compared to standard shunt catheter (non-AISC) from 8.13% to 4.09% (odds ratio [OR], 0.48; 95% confidence interval [CI], 0.40-0.58; p=0.01; I2=46%). Subgroup analysis of different age groups showed that AISC had significant antimicrobial effects in all three groups (adult, infant, and adolescent). Follow-up time analysis showed that AISC was effective in preventing early shunt infections (within 6 months after implant). AISC is more effective in high-risk population (OR, 0.24;95% CI, 0.14-0.40; p=0.60; I2=0%) than in general patient population. Conclusion : The results of meta-analysis indicated that AISC is an effective method for reducing shunt infection. We recommend that AISC should be considered for use in infants and high-risk groups. For adult patients, the choice for AISC could be determined based on the treatment cost.

Comprehensive Analysis of Vascular Endothelial Growth Factor-C Related Factors in Stomach Cancer

Liu, Yong-Chao,Zhao, Jing,Hu, Cheng-En,Gan, Jun,Zhang, Wen-Hong,Huang, Guang-Jian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Background: Vascular endothelial growth factor-C (VEGF-C), which contributes to lymphatic metastasis (LM) in malignant disease, is one of the most important factors involved in physical and pathological lymphangiogenesis. Some VEGF-C related factors such as sine oculis homeobox homolog (SIX) 1, contactin (CNTN) 1 and dual specificity phosphatase (DUSP) 6 have been extensively studied in malignancies, but their expression levels and associations have still to be elucidated in stomach cancer. Methods: We detected their expression levels in 30 paired stomach cancer tissues using quantitative real-time reverse transcription-PCR (qRT-PCR). The expression and clinical significance of each factor was analyzed using Wilcoxon signed rank sum test. The correlation among all the factors was performed by Spearman rank correlation analysis. Results: The results suggest that VEGF-C and CNTN1 are significantly correlated with tumor size, SIX1 with the age and CNTN1 also with the cTNM stage. There are significant correlations of expression levels among VEGF-C, SIX1, CNTN1 and DUSP6. Conclusions: There exists an important regulatory crosstalk involving SIX1, VEGF-C, CNTN1 and DUSP6 in stomach cancer.

Investigation on Microstructural Delamination and Compositional Segregation in Flange Steel with a High Stretch Ratio

Wen Liang,Qing Yuan,Sheng Liu,Guang Xu 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.6

In order to reveal the formation mechanism of microstructural delamination in flange steel during punching, the microstructuralcharacteristics of flange steels with 0.064, 0.085, and 0.101 wt% carbon contents were analyzed by an opticalmicroscope, optical emission spectrometer, metal in situ analyzer, and electron probe microanalyzer. It was found that microstructuraldelamination occurred due to C and Mn segregation, especially the former. The intensive segregation degree ofC was dependent on its relatively smaller partition coefficient. Moreover, the segregation degree gradually decreased withthe decreasing carbon content. And the increased segregations of elements except carbon were related to the coupling effectbetween different alloying elements. Furthermore, C and Mn were prone to segregate at the 1/4th position along the transversedirection of the casting slab; hence, almost no segregation was noticed at the edge of the casting slab. The homogenizationtreatment had a substantial effect on the elimination of C segregation; however, Mn segregation was relatively hard to beremoved.

Risk Factors for the Rupture of Bifurcation Intracranial Aneurysms Using CT Angiography

Li Wen,Guang-xian Wang,Dong Zhang,Zhi-ping Wang,Liu-qing Yang,Lei Zhang 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.5

Purpose: To investigate the clinical and morphological characteristics in relation to risk of bifurcation intracranial aneurysm rupture. Materials and Methods: Data from 202 consecutive patients with 219 bifurcation aneurysms (129 ruptured and 90 unruptured) managed at the authors’ facility between August 2011 and July 2014 were retrospectively reviewed. Based on their clinical records and CT angiographic findings, the ability of risk factors to predict aneurysm rupture was assessed using statistical methods. Results: Age, hypertension, diabetes mellitus, and cerebral atherosclerosis were negatively correlated with aneurysm rupture. Aneurysms located in the middle cerebral artery, daughter artery ratio, lateral angle ratio (LA ratio), and neck width were negativelycorrelated with rupture. Aneurysms located in the anterior communicating artery, irregularity, with daughter sac, depth, width, maximum size, aspect ratio (AR), depth-to-width ratio, and bottleneck factor were significantly and positively correlated with rupture. Binary logistic regression model revealed that irregular shape [odds ratio (OR) 6.598] and AR (OR 3.507) strongly increasedthe risk of bifurcation aneurysm rupture, while age (OR 0.434), cerebral atherosclerosis (OR 0.125), neck width (OR 0.771), and LA ratio (OR 0.267) were negatively correlated with rupture (p<0.05). Receiver operating characteristic analysis revealedthe threshold values of AR and LA ratio to be 1.18 and 1.50, respectively. Conclusion: Age (≥60 yr), cerebral atherosclerosis, and aneurysms with a larger neck width and larger LA ratio are protective factorsagainst bifurcation aneurysm rupture. An aneurysm with an irregular shape and an increased AR reflect the greater likelihoodof a rupture.

X-Ray crystal structure of [HSm{V^IVO(TPPS)}]_<i>n</i> and encapsulation of nitrogen molecules in 1-D channels

Chen, Wen-Tong,Yamada, Yusuke,Liu, Guang-Ning,Kubota, Akira,Ichikawa, Takayuki,Kojima, Yoshitsugu,Guo, Guo-Cong,Fukuzumi, Shunichi Royal Society of Chemistry 2011 Dalton transactions Vol.40 No.48

<P>The crystal structure of an N<SUB>2</SUB>-encapusulated MOF, which is stable under open-air conditions at ambient temperature, was determined by single-crystal X-ray diffraction at 123 K. The crystal MOF of [HSm{V<SUP>IV</SUP>O(TPPS)}]<SUB><I>n</I></SUB> designed to have 1-D channels periodically constricted by porphyrins planes adsorbed N<SUB>2</SUB> at 77 K. The adsorbed N<SUB>2</SUB> molecules remained in the 1-D channels even after warming to ambient temperature. The single-crystal structure of [HSm{V<SUP>IV</SUP>O(TPPS)}]<SUB><I>n</I></SUB>⊃N<SUB>2</SUB> determined by X-ray diffraction indicated that N<SUB>2</SUB> molecules trapped in the constricted parts block other N<SUB>2</SUB> molecules in 1-D channels from escaping from the MOF. Such a unique encapsulation mode provides a promising approach toward designing novel MOFs with high gas storage capacity at ambient temperature.</P> <P>Graphic Abstract</P><P>The shrinking of a channel window to fit the size of stopper molecules enables encapsulation of adsorbates in the 1-D channels. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1dt10956a'> </P>

<i>Haloactinopolyspora alkaliphila</i> sp. nov., and emended description of the genus <i>Haloactinopolyspora</i>

Zhang, Yong-Guang,Liu, Qing,Wang, Hong-Fei,Zhang, Dao-Feng,Zhang, Yuan-Ming,Park, Dong-Jin,Kim, Chang-Jin,Li, Wen-Jun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.6

<P>A facultatively alkaliphilic actinomycete strain, designated EGI 80088<SUP>T</SUP>, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088<SUP>T</SUP> formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained <SMALL>ll</SMALL>-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, summed feature 4 (iso-C<SUB>17 : 1</SUB>I/anteiso-C<SUB>17 : 1</SUB>B), iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The predominant menaquinone was MK-9(H<SUB>4</SUB>). The G+C content of the genomic DNA of strain EGI 80088<SUP>T</SUP> was 70.6 mol%. EGI 80088<SUP>T</SUP> showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour <I>Haloactinopolyspora alba</I> YIM 93246<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088<SUP>T</SUP> and <I>H. alba</I> YIM 93246<SUP>T</SUP> was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088<SUP>T</SUP> represents a novel species of the genus <I>Haloactinopolyspora</I>, for which the name <I>Haloactinopolyspora alkaliphila</I> sp. nov. (type strain EGI 80088<SUP>T</SUP> = BCRC 16946<SUP>T</SUP> = JCM 19128<SUP>T</SUP>) is proposed. The description of the genus <I>Haloactinopolyspora</I> has also been emended.</P>

Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

Zhang, Jing-Wen,Yu, Wan-Jia,Sheng, Xiao-Min,Chang, Fu-Hou,Bai, Tu-Ya,Lv, Xiao-Li,Wang, Guang,Liu, Su-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

BMB Reports : Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice

( Ai Ling Liu ),( Jie Zou ),( Cui Fang Liu ),( Xiao Yun Zhou ),( Xian Wen Zhang ),( Guang Yu Luo ),( Xin Bo Chen ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.1

Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings. [BMB Reports 2013; 46(1): 31-36]

Establishment and Analysis of Erosion Depth Model for Impeller Material FV520B

Zi-Wu Liu,Jian-Feng Li,Xiu-Jie Jia,Guang-Cun Wang,Wen-Han Xu 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.3 No.1

The multifactorial erosion was conducted in this paper to test the compressor impeller material FV520B using high-speed gas-solid two phase flow erosion tester and surface morphology analysis method. Based on the particle motion and collision energy equation as well as regression analysis of multi-factor orthogonal experiment, a phenomenological erosion depth model which captures the effects of impact velocity, angle and particle size, has been developed. The model includes removal of material due to both deformation damage and micro-cutting. Results show that the peak of experiment depth and the maximum calculated depth all appeared at near 45o, rather than near 24o where the maximum erosion rate appeared. Comparing the calculated values and the results of each single factor experiment, the errors are within 15%. The predictions of the simplified version of the model were in good agreement with the results of single factor experiments. Also, the reliability of the assessment formula was verified to assess the impeller erosion life, which indicated that this calculation model could be used to estimate the erosion depth of compressor impeller material FV520B.