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Measurement of antibacterial properties of foil‑backed electrospun nanofibers
Mary Ann Wagner‑Graham,Herbert Barndt,Mark Andrew Sunderland 한국의류학회 2019 Fashion and Textiles Vol.6 No.1
Current methodologies for evaluation of antibacterial properties of traditional textiles are not applicable to foil-backed, poorly-absorbent electrospun nanofiber materials, since existing test methods require absorbent fabrics. Since electrospun nanofibers are adhered to the foil backing only by electrostatic interactions, methods used to evaluate antibacterial properties of surfaces cannot be used because these protocols cause the nanofibers to lift from the foil backing. Therefore, a novel method for measurement of the antibacterial properties of electrospun metallic foil-backed nanofiber materials was developed. This method indicated that acetate-based nanofibers manufactured to contain 5 to 30 weight percent of cold-pressed hemp seed oil or full-spectrum hemp extract inhibited the growth of Staphylococcus aureus in a dose-dependent manner, from 85.3% (SEM = 2.2) inhibition to 99.3% (SEM = 0.15) inhibition, respectively. This testing method represents an advanced manufacturing prototype procedure for assessment of antibacterial properties of novel electrospun, metallic foil-backed nanofiber materials.
Utilization of cryopreserved sperm cells based on the indication for storage
Graham Luke Machen,Stephanie E. Harris,Erin T. Bird,Monica L. Brown,Dale A. Ingalsbe,Milaida M. East,Michelle Reyes,Thomas J. Kuehl 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3
Purpose: Cryopreservation of sperm from human semen has been available since the 1950s. The actual utilization of available cryopreservation technology has been infrequently reported. We set out to examine the utilization and outcomes of cryopreserved sperm cells based on the indication for storage. Materials and Methods: A dataset was developed from retrospective review. The purposes for cryopreservation, eventual utilization, and outcomes of use for insemination were recorded. The types of utilization were accumulated as proportions for different purposes. The timing for use of samples for insemination procedures was evaluated using survival statistics. The frequency of patients arranging to destroy samples was also reported. Results: From September 1988 through March 2015, 1442 samples were cryopreserved. Samples were cryopreserved for four primary purposes: infertility treatments focused on intrauterine insemination (IUI) or in vitro fertilization/intracellular injection (IVF/ICSI), for fertility preservation related to cancer treatment, or prior to military deployment. Total utilization rates were 19.3%. Samples cryopreserved for IUI were more likely to be used (64.3%), while samples cryopreserved as backup for IVF/ICSI were more likely to be destroyed (29.8%). Pregnancy rates varied based on the indication and ART used. Pregnancies per cycle were 35% for IVF/ICSI and were 10% for IUI. Conclusions: Cryopreservation of sperm is a valuable and underutilized resource, particularly amongst male cancer patients. This technology can facilitate infertility treatments based on a variety of indications, including deployment—a patient cohort unique to our dataset.