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      • Evaluation of phenolic compounds and antimicrobial activities in transgenic Codonopsis lanceolata plants via overexpression of the γ-tocopherol methyltransferase (γ-tmt) gene

        Ghimire, B.K.,Seong, E.S.,Yu, C.Y.,Kim, S.H.,Chung, I.M. Bureau for Scientific Publications, Foundation for 2017 South African journal of botany : official journal Vol.109 No.-

        <P>This study was carried out to investigate antimicrobial activities of leaf and root extracts of transgenic Codonopsis lanceolata with over-expressing gamma-tocopherol methyl transferase (gamma-tmt) gene. The antimicrobial activity was assessed using the agar disc diffusion method and minimum inhibitory concentration (MIC) method, and the extracts from the transgenic C. lanceolata were compared with those of the wild plants by using the in-vitro antimicrobial assays with five pathogenic bacteria and two yeast species. The results indicated that transgenic C. lanceolata overexpressing gamma-tmt possesses antimicrobial activity against both gram-positive and gram-negative bacteria. Transgenic lines showed increased antimicrobial activity, ranging from 1.90 +/- 0.50 to 5.50 +/- 0.77 mm in the leaf extract of transgenic C. lanceolata, compared to the extract from the control plants, ranging from 1.27 +/- 0.50 to 4.73 +/- 0.40 mm. Staphylococcus aureus was the most susceptible microorganism to transgenic leaf extracts with lowerMICs (500 mu g/mL) and higher growth inhibition zones (5.33mm). Leaf extract of transgenic plants showed antimicrobial activity against Salmonella typhimurium, Klebsiella pneumoniae, and Escherichia coli. However, transgenic root extract showed lower antimicrobial activity against all tested microorganisms, with MIC > 1000 mu g/mL. The a-tocopherol content of the plants was enhanced by the overexpression of (gamma-tmt) gene compared to control plant. We observed an increase in the phenolic compound concentration in transgenic leaves. The predominant phenolic compounds that increased in the transgenic leaf were chlorogenic acid, luteolin, benzoic acid, and apigenin. The antimicrobial activity of transgenic C. lanceolata was strongly correlated to the concentration of phytochemical compounds. These results may help to identify novel natural antimicrobial agents that could be used against infectious diseases. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved.</P>

      • Nonlinear transfer function-based local approach for color image enhancement

        Ghimire, D.,Joonwhoan Lee IEEE 2011 IEEE TRANSACTIONS ON CONSUMER ELECTRONICS - Vol.57 No.2

        <P>The main objective of image enhancement is to improve some characteristic of an image to make it visually better one. This paper proposes a method for enhancing the color images based on nonlinear transfer function and pixel neighborhood by preserving details. In the proposed method, the image enhancement is applied only on the V (luminance value) component of the HSV color image and H and S component are kept unchanged to prevent the degradation of color balance between HSV components. The V channel is enhanced in two steps. First the V component image is divided into smaller overlapping blocks and for each pixel inside the block the luminance enhancement is carried out using nonlinear transfer function. In the second step, each pixel is further enhanced for the adjustment of the image contrast depending upon the center pixel value and its neighborhood pixel values. Finally, original H and S component image and enhanced V component image are converted back to RGB image. The subjective and objective performance evaluation shows that the proposed enhancement method yields better results without changing image original color in comparison with the conventional methods.</P>

      • SCISCIESCOPUS

        Anisotropic lattice disorder and enhanced magnetic anisotropy in Fe<sub>3</sub>O<sub>4</sub> films on (1 1 0) SrTiO<sub>3</sub>

        Ghimire, Santosh,Dho, Joonghoe Elsevier 2018 Journal of magnetism and magnetic materials Vol.468 No.-

        <P><B>Abstract</B></P> <P>Epitaxial Fe<SUB>3</SUB>O<SUB>4</SUB> films on (1 1 0)-oriented SrTiO<SUB>3</SUB> and MgAl<SUB>2</SUB>O<SUB>4</SUB> were prepared to investigate the in-plane-direction dependence of the interfacial lattice disorder and its effect on the magnetic property. Transmission electron microscopy images of (1 1 0) Fe<SUB>3</SUB>O<SUB>4</SUB>/SrTiO<SUB>3</SUB> showed that lattice spots at the interface along the in-plane [ −1 1 0] direction were quite regular, but those along the in-plane [0 0 1] direction exhibited a distinctive lattice disorder. In contrast, the (1 1 0) Fe<SUB>3</SUB>O<SUB>4</SUB>/MgAl<SUB>2</SUB>O<SUB>4</SUB> exhibited quite regular and smooth interfaces along both in-plane directions. The (1 1 0) Fe<SUB>3</SUB>O<SUB>4</SUB>/SrTiO<SUB>3</SUB>, which keeps structural symmetry across the interface along the in-plane [ −1 1 0] direction but not across the interface along the in-plane [0 0 1] direction, exhibited improved properties: The in-plane uniaxial magnetic anisotropy was about five times larger than those in the previous reports, and the maximum magnetoresistance was about twice that of the Fe<SUB>3</SUB>O<SUB>4</SUB>/MgAl<SUB>2</SUB>O<SUB>4</SUB>. This suggests that the microscopically anisotropic lattice disorder at the interface can be used to control the uniaxial magnetic anisotropy and the magnetoresistance of a magnetic film for spintronic applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> (1 1 0) Fe<SUB>3</SUB>O<SUB>4</SUB>/SrTiO<SUB>3</SUB> exhibited the in-plane-direction dependence of interfacial lattice disorder. </LI> <LI> Its interfacial lattice along the in-plane [ −1 1 0] direction was regular, but not along the [0 0 1]. </LI> <LI> Its in-plane uniaxial magnetic anisotropy was five times larger than those in previous reports. </LI> <LI> Its magnetoresistance was about twice that of the Fe<SUB>3</SUB>O<SUB>4</SUB>/MgAl<SUB>2</SUB>O<SUB>4</SUB> with smooth interface. </LI> </UL> </P>

      • Improved antioxidant activity in transgenic Perilla frutescens plants via overexpression of the γ-tocopherol methyltransferase (γ-tmt) gene.

        Ghimire, Bimal Kumar,Seong, Eun Soo,Lee, Chan Ok,Lee, Jae Geun,Yu, Chang Yeon,Kim, Seung Hyun,Chung, Ill Min Springer 2015 Protoplasma Vol.252 No.5

        <P>The main goal of this study was to generate transgenic Perilla frutescens with enhanced antioxidant properties by overexpressing the γ-tocopherol methyltransferase (γ-tmt) gene. In this study, the antioxidant activity of methanolic crude extracts of transgenic and non-transgenic control plants was investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Free radical scavenging activity was evaluated using α-tocopherol and butylated hydroxyl toluene as standard antioxidants. In general, the ethyl acetate fraction of transgenic P. frutescens showed stronger DPPH radical scavenging activity than the ethyl acetate fraction from non-transgenic control plants (IC50 2.00??0.10 and 5.53??0.40?μg?ml(-1), respectively). High-performance liquid chromatography analysis of phenolic acids in leaf extracts confirmed increased levels of 16 individual phenolic compounds in two transgenic lines (pf47-5 and pf47-8) compared with control plants. Changes in the phenolic compound profile and α-tocopherol content were correlated with the antioxidant properties of transgenic plants, indicating that the introduction of transgene γ-tmt influenced the metabolism of phenolic compounds and subsequently produced biochemical changes in the transformants. There were no significant differences in photosynthetic rate in the transgenic plants as compared to the non-transgenic control plants, suggesting that the alteration of phenolic compounds and tocopherol composition had little impact on photosynthesis.</P>

      • Assessment of the phenolic profile, antimicrobial activity and oxidative stability of transgenic <i>Perilla frutescens</i> L.overexpressing tocopherol methyltransferase (<i>γ-tmt</i>) gene

        Ghimire, Bimal Kumar,Yu, Chang Yeon,Chung, Ill-Min Elsevier 2017 Vol. No.

        <P><B>Abstract</B></P> <P>This study evaluated the effects of enhanced concentrations of α-tocopherol and phenolic compounds on the resistance and stability of Perilla oil in transgenic <I>Perilla frutescens</I> plants against various tested pathogenic bacteria by over-expressing the <I>γ-tmt</I> gene. The concentration of phenolic compounds in the non-transgenic samples was 9313.198 ± 18.887 μg g<SUP>−1</SUP> dry weight (DW), whereas the total concentration of the transgenic samples ranged from 9118.015 ± 18.822 to 10527.612 ± 20.411 μg g<SUP>−1</SUP> DW. The largest increases in phenolic compounds in the transgenic plants in comparison with the control plants were observed in gallic acid, pyrogallol, 5-sulfosalicylic acid, catechin, chlorogenic acid, vanillin, syringic acid, naringenin, salicylic acid, quercetin, <I>o</I>-coumaric acid, kaempferol, and hesperetin. <I>o</I>-coumaric and benzoic acid acid were the most abundant phenolic acids found in the transgenic plants. Gram-negative bacteria (<I>Salmonella typhimurium</I>) were the most susceptible microorganism against transgenic ethyl acetate extracts with lower measurement of minimum inhibitory concentration <B>(</B>MICs) (0.25 ± 0.03 mg/ml) at an extract concentration of 2 mg/ml in dried plant material. The same extracts were more effective against gram-positive bacteria (<I>Bacillus subtilis</I>) when compared to control plants with MICs values of 0.52 ± 0.02 mg/ml. The suplementation of 20 μg of α-tocopherol (1000 ppm) in combination with ethyl acetate extracts enhanced the antimicrobial activity against <I>S. typhimurium</I> and <I>B. subtilis,</I> compared to the non-transgenic plants. The acid value of transgenic Perilla oil improved by 91.2% and 35.54% relative to the non-transgenic control oil and commercial Perilla oil, respectively. The low acid value suggests that the oil will be less susceptible to lipase action, and more economically viable and thus, may also improve the oil quality for industrial purposes. In addition, extracts obtained from transgenic plants could be a potential source of antimicrobial agents for the treatment of bacterial infections.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Higher phenolic compounds in the transgenic plants in comparison with the control plants. </LI> <LI> Benzoic acid and <I>o</I>-coumaric acid were higher in the transgenic plants. </LI> <LI> Gram-negative bacteria (<I>Salmonella typhimurium</I>) were susceptible against transgenic ethyl acetate extracts. </LI> <LI> The ethyl acetate extracts were more effective against <I>Bacillus subtilis.</I> </LI> </UL> </P>

      • Data-Driven Intelligent Feeding System for Pet Care

        Ghimire Ravi,Jae Weon Choi 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11

        The rapid development of artificial intelligence, the internet of things, and digital information processing technology has a huge impact on our daily lives with smart devices and wearables. The well-being of companion animals such as dogs and cats has become a large challenge. An increasing number of pet owners, their emotional attachment with their pets, and the 21st-century’s lifestyle importantly need the safety and welfare of pets by harnessing a smart technological approach. This paper analyzes and compares different machine learning algorithms for data-driven intelligent feeding system for pet care application. Different parameters such as body weight growth, body temperature, heart rate, eating habits, activity, sleep, and urine pH have been considered with other correlated sub-variables in creating virtual datasets. The supervised machine learning models: linear regression, gaussian process regression, narrow neural network, linear support vector machine, and fine tree are evaluated and discussed for estimating feed quantity. The machine learning model was verified by training, validation, and testing datasets. The developed model will be an innovative breakthrough for pet care applications. Feed estimation can be automated using the pet’s health parameters, this will help the pet to prevent obesity and related disorders.

      • Graphene-CdSe quantum dot hybrid as a platform for the control of carrier temperature

        Ghimire, Mohan Kumar,Gul, Hamza Zad,Yi, Hojoon,Dang, Dang Xuan,Sakong, Won Kil,Luan, Nguyen Van,Ji, Hyun Jin,Lim, Seong Chu Elsevier 2017 Flatchem Vol.6 No.-

        <P><B>Abstract</B></P> <P>In a graphene and quantum dots (QDs) hybrid structure, the graphene is known to play the role of an electrode that conducts the photoexcited carriers from the QDs to the electrodes. Thus, the yield of photocurrent of the QD ensemble is greatly enhanced . However, in our study, the graphene provides a platform to control the energy relaxation of optically excited carriers from QDs. Thus, the temperature of photocarriers of QDs is controllable. Due to the moderate carrier temperature, the observed photocurrent from the hybrid structure reveals a photothermoelectric effect, which becomes even stronger when the Fermi energy, E<SUB>F</SUB>, is located near the charge neutrality point (CNP) of the graphene. However, the photothermoelectric behavior weakens with increased E<SUB>F</SUB>. Such a behavior originates from the varying electron-phonon coupling strength that is dependent upon E<SUB>F</SUB> of the graphene.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In a graphene, the coupling strength of electron and optical phonon can be controlled by electrostatic gating. </LI> <LI> Hybridization of graphene and QDs provides a platform that we can control the carrier temperature. </LI> <LI> The carrier temperature ranges 0.03 to 1.6K above the graphene lattice temperature depending on the back gate bias. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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