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        Evaluation of Drought Tolerance in Tef [Eragrostis Tef (Zucc.) Trotter] Genotypes Using Drought Tolerance Indices

        Brikti Ferede,Firew Mekbib,Kebebew Assefa,Solomon Chanyalew,Eyasu Abraha,Zerihun Tadele 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.2

        A field experiment was conducted to identify drought tolerant, high yielding and stable tef genotypes using drought tolerance indices. Sixty-four tef genotypes were evaluated under drought and non-stress conditions using “8 x 8 simple lattice design” with two replications at Debre-Zeit Agricultural Research Center. Drought tolerance indices including drought susceptibility index (SSI), drought tolerance index (STI), geometric mean productivity (GMP), mean productivity (MP), yield index (YI), yield stability index (YSI), drought resistance index (DRI), abiotic tolerance index (ATI), stress susceptibility percentage index (SSPI), harmonic mean (HM) were estimated from grain yield under drought (Ys) and non-stress (Yp) conditions. Highest grain yields in both drought and normal watering conditions were recorded for genotype Melko. Based on drought tolerant indices the highest values of STI, GMP, and MP were related to genotypes Melko, Etsub, and Ziquala. Correlation analysis revealed that grain yield was positively and significantly correlated with STI, GMP, MP, YI, DRI, and HM, indicating that these indices were more important for screening stress-tolerant tef genotypes. Principal component analysis indicated that the first two components accounted for more than 98% of the total variations. The first PC contributed for 67.9% of the variation with strong presence of STI, GMP, MP, YI, DRI, HM, Ys, and Yp. Biplot analysis also indicated the STI, GMP, MP, YI, DRI, and HM were more reliable indices to identify drought tolerant tef genotypes. Cluster analysis grouped the 64 tef genotypes into seven clusters. The study showed that selection based on indices which exhibited significant and positive coordination with Ys and Yp are more reliable parameters for breeders to use in the tef improvement program.

      • KCI등재후보

        Molecular Diversity and Population Structure of the Ethiopian Lentil (Lens Culinaris Medikus) Genotype Assessment Using SSR Markers

        Fikru Mekonnen,Firew Mekbib,Shiv Kumar,Seid Ahmed,TR Sharma 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.1

        Knowledge of genetic diversity in germplasm is essential for formulating effective germplasm collection, conservation, utilization strategies in and crop improvement programs. It also provides an opportunity to take corrective steps infusing new genes to avoid risks associated with a narrow genetic bases. Genetic diversity analysis of 119 lentil genotypes of including 83 germplasm and 36 exotic genotypes from International Center for Agricultural Research in the Dry Areas was studied using 27 primers of simple sequence repeat (SSR) marker. Molecular analysis of variance showed variations of 82% within and 18% of the among population variance was explained. Degree of polymorphism observed among the populations was 100%. A total 122 alleles were detected, with 2 to 7 alleles per locus, with a mean of 4.52 alleles per locus. The estimated gene diversity value for 27 loci was 0.64. The average Shannon’s information index value of 1.19 was obtained showed the existence of high genetic variation within the genotypes. The genetic similarity indices ranged from 0.21 to 1.00. The SSR markers showed an average polymorphic information content (PIC) value of 0.58. Cluster analysis grouped the genotypes into five major clusters as distinct genetic populations. Diversity analyses revealed the existence of a high level of genetic variation among genotypes. This molecular diversity information provides a basis for future germplasm collection, utilization, and conservation strategies in gene banks and introducing exotic germplasm to widen the genetic base of the current lentil breeding population.

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        Genotype × environment interaction and yield stability in barley (Hordeum vulgare L.) genotypes in the central highland of Ethiopia

        Fekadu Wondimu,Mekbib Firew,Lakew Berhane,Haussmann Bettina I. G. 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2

        Barley is one of the most important cereal crops cultivated over a wider environment in the diverse agro-ecologies in Ethiopia. Study on genotype by environment interaction and stability analysis using thirty barley genotypes was conducted across nine environments in randomized complete block design with three replications to study the magnitude of genotype by environment interaction and to evaluate the stability and adaptability of barley genotypes with high mean yield performance. Additive main efect and multiplicative interaction (AMMI), genotype main efect and genotypes by environment interaction (GGE) and Eberhart and Russell models were employed for stability and adaptability analysis. Thus, AMMI analysis of variance for grain yield showed highly signifcant (P≤0.001) diferences due to genotypes, environment and genotype by environment interaction. Accordingly, environment accounted for (54.61%) of the total variations followed by genotype (10.69%) and G×E interaction (34.70%). Moreover, a substantial percentage of the G×E interaction sum of squares was explained by IPCA1 (45.48%) followed by IPCA2 (24.65%) and IPCA3 (13.02%) while the frst two IPCAs explained 70.13%. Moreover, AMMI and GGE were found to be efcient in grouping the barley growing environment in the central highland, whereas Debre Markos and Bekoji were good representative testing environments. Generally, the current study indicated that 3514-A, 24,990, and 17,148 were desirable genotypes for subsequent breeding line identifcation and variety development.

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