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LIN Lei,FU Xiaoyun,GAO Haiping 유공압건설기계학회 2015 유공압건설기계학회 학술대회논문집 Vol.2015 No.10
Installing hydraulic mufflers in piping system is one of the most extensive methods to attenuate the fluid pressure pulse and noise. In order to refine the current numerical analysis model for hydraulic mufflers, the fluid-structure interaction mathematical model is analyzed, and a two-dimension transient model of fluid-structure interaction is established and calculated by ADINA. By the analysis of the simulation result, the law of pressure fluctuation attenuation varied with frequency is obtained. The analysis shows that the simulation results are very consistent with experimental results completed by other researchers, and verify the numerical simulation. On this basis, the relationships between the structure parameters and the noise attenuation have been analyzed in order to realize the structure optimization. In this paper, the research works have made a contribution for more accurate analysis the performance of hydraulic muffler.
Liu, Bijin,Fu, Danjuan,Zhang, Youquan,Chen, Xiaoyun The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.1
Taking the cylindrical float of the floating fence of a floating litter collection device as the research object, based on the shallow immersion characteristics of the cylindrical float, the Morison equation is modified, and the interaction between regular waves and the partially immersed horizontal cylindrical float is discussed in combination with scale model test. The results show that the modified Morison equation can accurately predict the wave force of the horizontal cylindrical float and reveal the influence of amplitude, immersion depth and period on the wave force of the cylindrical float. For partially immersed cylindrical floats, the wave force increases with the increase in wave height and decays with the increase in period. The positive value distribution of the wave force is larger than that of the negative direction, and the difference between the positive and negative directions is mainly affected by the immersion depth.
Liu, Bijin,Fu, Danjuan,Zhang, Youquan,Chen, Xiaoyun The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-
Taking the cylindrical float of the floating fence of a floating litter collection device as the research object, based on the shallow immersion characteristics of the cylindrical float, the Morison equation is modified, and the interaction between regular waves and the partially immersed horizontal cylindrical float is discussed in combination with scale model test. The results show that the modified Morison equation can accurately predict the wave force of the horizontal cylindrical float and reveal the influence of amplitude, immersion depth and period on the wave force of the cylindrical float. For partially immersed cylindrical floats, the wave force increases with the increase in wave height and decays with the increase in period. The positive value distribution of the wave force is larger than that of the negative direction, and the difference between the positive and negative directions is mainly affected by the immersion depth.
Rapid Detection of Clostridium tetani by Recombinase Polymerase Amplification Using an Exo Probe
( Mingjing Guo ),( Pan Feng ),( Liqun Zhang ),( Chunfeng Feng ),( Jie Fu ),( Xiaoyun Pu ),( Fei Liu ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1
Tetanus is a potentially fatal public health illness resulted from the neurotoxins generated by Clostridium tetani. C. tetani is not easily culturable and culturing the relevant bacteria from infected wounds has rarely been useful in diagnosis; PCR-based assays can only be conducted at highly sophisticated laboratories. Therefore, a real-time recombinase polymerase amplification assay (Exo-RPA) was constructed to identify the fragments of the neurotoxin gene of C. tetani. Primers and the exo probe targeting the conserved region were designed, and the resulting amplicons could be detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in penetrating wounds. Tests of target-spiked serum and pus extract revealed that RPA is robust to interfering factors and has great potential for further development for biological sample analysis. This method has been confirmed to be reliable for discriminating between toxic and nontoxic C. tetani strains. The RPA assay dramatically improves the diagnostic efficacy with simplified device architecture and is a promising alternative to real-time PCR for tetanus detection.