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알지네이트 이용한 체내기생봉의 산란과 사육을 위한 인공알 제조
조은비 ( Eunbee Cho ),홍기정 ( Gi-jeong Hong ),선우훈 ( Hoon Seonwoo ) 한국농업기계학회 2021 한국농업기계학회 학술발표논문집 Vol.26 No.1
In order to control pests such as aphids, a method of treating insecticides on infected crops is widely used, which causes damage such as environmental pollution, elimination of beneficial insects, and increased resistance to pests. To solve this problem, alternatives to pesticides are being studied, one of which is the use of biological control factors such as predator and parasitoids. In this study, in order to provide an artificial host for continuous breeding of endoparasitoids, artificial eggs of similar size to natural hosts were mass-produced. Using a high voltage power supply, voltage is applied to the syringe containing the artificial medium-alginate solution and the calcium chloride solution. Then, a drop of the artificial medium-alginate solution was dropped into the calcium chloride solution using a syringe pump to produce beads. The injectability was highest when the ratio of artificial medium and alginate solution was 1:0.25 and when a voltage of 5kV was applied, the beads were formed at a constant size of 1mm, similar to their natural hosts, and mass production was possible in a short time. If mass breeding of endoparasitoids is possible through these artificial eggs, it can be an effective way to reduce the damage of environmental pollution while controlling pests.
꼬막 껍질 파우더를 기반으로 한 3D 프린팅이 가능한 칼슘 인산염 시멘트 개발
조은비 ( Eunbee Cho ),굴노즈 ( Hasanova Gilnoz Olimovnar ),이성민 ( Sungmin Lee ),선우훈 ( Hoon Seonwoo ) 한국농업기계학회 2022 한국농업기계학회 학술발표논문집 Vol.27 No.2
Calcium Phosphate Cements (CPCs) scaffolds fabricated by three-dimensional (3D) printing are increasingly used of bone tissue repair. In general, the production rate of CPC is low because the amount of calcium phosphate compounds in existing raw materials such as cow bone and pork bone are small. On the other hand, cockle shells contain more than 99% of CaCO3, the main component of CPC, in the form of amorphous aragonite with excellent bioavailability. Therefore, in this study, Cockle shell powders-based CPC (CSP-CPCs) was produced by extrusion method using nozzles than the conventional nozzle for CPC 3D printing. The printability was improved by adding lactic acid and hyaluronic acid. We investigated the biocompatibility of the CSP-CPCs scaffolds using WST-1and Live/Dead were conducted, as well. In addition, CSP-CPCs were mounted on the mouse of the calvarial defect model to confirm bone regeneration. It is possible to create high added value in fishing villages by recycling natural products thrown away as marine waste through this method.
꼬막 껍질 파우더와 β-TCP를 기반으로 한 3D 프린팅이 가능한 칼슘 인산염 시멘트 개발
조은비 ( Eunbee Cho ),이주오 ( Juo Lee ),선우훈 ( Hoon Seonwoo ) 한국농업기계학회 2021 한국농업기계학회 학술발표논문집 Vol.26 No.2
Calcium Phosphate Cement(CPC) scaffolds fabricated by 3D printing are increasingly used in the field of bone tissue repair. Generally, production rate of CPCs are low since the amount of calcium phosphate compounds is small in conventional raw materials, such as bovine bone and porcine bone. On the other hand, cockle shell contains more than 99% of CaCO3, one of the main constituents of CPC, in the form of amorphous aragonite which has excellent bioavailability. Therefore, in this study, cockle shell powder and β-TCP based CPC scaffolds were fabricated through an extrusion method using nozzles which are narrower than conventional one for CPC 3D printing. Their morphology and biocompatibility were investigated. With this method, high-added value can be created for fishing villages due to the recycling of natural materials which is thrown away as marine waste.
꼬막 껍질 파우더를 기반으로 한 3D 프린팅이 가능한 칼슘 인산염 시멘트 개발
조은비 ( Eunbee Cho ),굴노즈 ( Hasanova Gilnoz Olimovnar ),이성민 ( Sungmin Lee ),선우훈 ( Hoon Seonwoo ) 한국농업기계학회 2022 한국농업기계학회 학술발표논문집 Vol.27 No.2
Calcium Phosphate Cements (CPCs) scaffolds fabricated by three-dimensional (3D) printing are increasingly used of bone tissue repair. In general, the production rate of CPC is low because the amount of calcium phosphate compounds in existing raw materials such as cow bone and pork bone are small. On the other hand, cockle shells contain more than 99% of CaCO3, the main component of CPC, in the form of amorphous aragonite with excellent bioavailability. Therefore, in this study, Cockle shell powders-based CPC (CSP-CPCs) was produced by extrusion method using nozzles than the conventional nozzle for CPC 3D printing. The printability was improved by adding lactic acid and hyaluronic acid. We investigated the biocompatibility of the CSP-CPCs scaffolds using WST-1and Live/Dead were conducted, as well. In addition, CSP-CPCs were mounted on the mouse of the calvarial defect model to confirm bone regeneration. It is possible to create high added value in fishing villages by recycling natural products thrown away as marine waste through this method.
공액화 고분자-그래핀 옥사이드 복합체의 턴-온 형광 변화를 이용한 세슘 이온 감지
장근석(Geunseok Jang),조은비(Eunbee Cho),Laurence M. Harwood,이택승(Taek Seung Lee) 한국고분자학회 2019 폴리머 Vol.43 No.2
세슘 이온의 검출과 함께 형광이 증가하는 턴-온 형광센서를 공액화 고분자(CP)와 그래핀 옥사이드(GO)로 구성된 복합체로 제조하였다. 세슘 이온과 상호작용을 위해 CP의 곁사슬에 테트라에틸렌 글라이콜(TEG)기를 도입하였다. GO는 유기분자와 소수성 상호작용하여 소수성인 CP와 쉽게 복합체를 형성하는 특성을 가지며, 복합체를 형성하면 CP에서 GO로 에너지 전달이 일어나 CP의 형광이 사라지게 된다. CP-GO 복합체가 세슘 이온에 노출되면 CP의 곁사슬인 테트라에틸렌 글라이콜과 세슘 이온이 결합하고, 이로 인해 CP와 GO의 상호작용이 약회되어 CP가 GO로부터 유리되며, 이탈된 CP는 원래의 형광을 회복하게 된다. 이는 세슘 이온을 선택적으로 감지하는 형광 턴-온 센서로 사용가능하게 된다. New type of “turn-on” fluorescent sensor for Cs ion was developed using a composite of blue-emissive conjugated polymer (CP) and graphene oxide (GO). Ethylene glycol units were introduced to the side chain of CP, which could interact with Cs ions. The CP and GO formed a stable, nonfluorescent composite, because of hydrophobic and π-π interactions between them. In the presence of Cs ions, the CP was released from the CP-GO composite because of higher affinity of Cs ions with ethylene glycol units in the CP. Such a weaker interaction between CP and GO led to the recovery of initial blue fluorescence of the CP. The degree of fluorescence restoration would be a measure of the concentration of Cs ions. Thus, fluorescence “turn-on” from CP-GO was attributed to the presence of Cs ions.