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자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염
박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2
경상북도 영덕군의 하천에 서식하는 점몰개 spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon)의 근육에서 새인두흡충 Clinostomum complanatum (Rudolphi, 1814)의 피낭유충이 발견되었다. 감염된 점몰개는 "yellow grub"인 기생충의 피낭유충으로 인해 표면이 울퉁불퉁하게 보였으며, 수면위에 힘없이 유영하였고 일부 폐사한 개체도 발견되었다. 분리된 탈낭유충의 형태는 혓바닥 모양이었으며, 크기는 2.85~5.89 mm×0.8~1.99 mm였다. 어체당 1~98 개의 피낭유충을 분리하였으며 평균 감염수는 25개로 나타났다. 특히, 성어크기인 평균 7 cm 정도의 점몰개는 100% 감염되어 있었다. 본 연구는 한국의 자연수계에 서식하는 어류에서 C. complanatum 의해 질병이 발생하고 폐사가 일어난 첫 보고이다. The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by "yellow grub" showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.
Jeon, Eugene,Hyeon, Jeong eun,Sung Eun, Lee,Park, Byeoung-Soo,Kim, Seung Woo,Lee, Jinwon,Han, Sung Ok Oxford University Press 2009 FEMS microbiology letters Vol.301 No.1
<P>In this study, Saccharomyces cerevisiae was engineered for simultaneous saccharification and fermentation of cellulose by the overexpression of the endoglucanase D (EngD) from Clostridium cellulovorans and the beta-glucosidase (Bgl1) from Saccharomycopsis fibuligera. To promote secretion of the two enzymes, the genes were fused to the secretion signal of the S. cerevisiaealpha mating factor gene. The recombinant developed yeast could produce ethanol through simultaneous production of sufficient extracellular endoglucanase and beta-glucosidase. When direct ethanol fermentation from 20 g L(-1)beta-glucan as a substrate was performed with our recombinant strains, the ethanol concentration reached 9.15 g L(-1) after 50 h of fermentation. The conversion ratio of ethanol from beta-glucan was 80.3% of the theoretical ethanol concentration produced from 20 g L(-1)beta-glucan. In conclusion, we have demonstrated the construction of a yeast strain capable of conversion of a cellulosic substrate to ethanol, representing significant progress towards the realization of processing of cellulosic biomass in a consolidated bioprocessing configuration.</P>
Jeon, Bo-Young,Kim, Seung-Cheol,Je, Sungmo,Kwak, Jeongyeon,Cho, Jang-Eun,Woo, Jong-Tae,Seo, Sangkyo,Shim, Hang-Sub,Park, Byoung-Ok,Lee, Sung-Sik,Cho, Sang-Nae Elsevier 2010 Research in veterinary science Vol.88 No.3
<P><B>Abstract</B></P><P>An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of <I>Mycobacterium bovis</I> was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (<I>R<SUP>2</SUP></I>=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively.</P><P>In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (<I>n</I>=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.</P>
Jeon, Sang Duck,Lee, Ji Eun,Kim, Su Jung,Kim, Seung Wook,Han, Sung Ok Elsevier 2012 Biosensors & Bioelectronics Vol.35 No.1
<P><B>Abstract</B></P> <P>Optical biosensors that use fluorescence are promising tools for the analysis of target materials such as protein, DNA and other biomaterial. To analyze the binding properties of a protein–protein interaction, we constructed fluorescent biomarkers based on the cohesin–dockerin interaction, which coordinates the assembly of cellulolytic enzymes and scaffolding proteins to produce a cell surface multiprotein complex known as the “cellulosome” in some anaerobic bacteria. Our 2D-PAGE results displayed diverse binding profiles to the dockerin containing cellulosomal proteins produced by <I>Clostridium cellulovorans</I> grown on different carbon sources, such as Avicel, xylan and AXP (Avicel:xylan:pectin (3:1:1)). Fluorescence intensity analysis indicated that EngE and EngH bound more efficiently to Coh6 than to Coh2 or Coh9 (2-fold to 6-fold and 1.5-fold to 5-fold, respectively), while others cellulosomal proteins displayed similar results. In addition, both an enzyme-linked interaction assay (ELIA) and surface plasmon resonance (SPR) analyses demonstrated that both EngE and EngH preferentially bound cohesin6 versus the other two cohesin molecules. This work demonstrated the analysis of the binding patterns between interacting proteins using fluorescent biomarkers. We also illustrated the potential of this sensitive approach to quantify specific target analytical materials via the example of the cohesin–dockerin interaction.</P> <P><B>Highlights</B></P> <P>▸ We constructed fluorescent cohesin markers selected by amino acid differences. ▸ Fluorescent intensity reflected the distinct interactions between cohesins and dockerin containing proteins in 2D-PAGE. ▸ Certain proteins had higher selective binding affinities than others, depending on the cohesin markers. ▸ ELIA and SPR analyses established quantitative binding affinity differences.</P>
Successful Brand Revitalization of Parkland through Brand Repositioning Strategy
Jeon, Jung Ok,Jung, Hyung-Shik,Lee, Sukekyu,Lee, Eun Mi Korean Marketing Association 2014 ASIA MARKETING JOURNAL Vol.16 No.3
Parkland, which is one of the pioneer brands in men's fashion in Korea, specifically suits, has recently undertaken bold brand repositioning activities to respond to the fast-changing environment and to overcome limitations in its current image positioning. As a result, in a short time period, Parkland achieved remarkable marketing and communication success. This case study explains how Parkland drew successful brand repositioning from the fierce fashion market. This study systematically analyzes the brand repositioning strategy process and implementation strategy used to resolve the conceptual and structural issues of Parkland as a mature brand. To this end, this study assesses Parkland's brand strategy focusing on brand environment and positioning. Accordingly, the study analyzes the target concept and basic direction of the brand repositioning in terms of the repositioning strategy process, and from an integrated marketing perspective, examines the specific implementation program for repositioning. Finally, the study addresses the outcomes of brand repositioning efforts as well as steps to be taken in the future.
Jeon, Jun Ho,Park, Deok-Bum,Woo, Sun-Je,Lee, Hae-Ri,Park, Ok-Kyu,Park, Jungchan,Rhie, Gi-eun Elsevier 2018 Cytokine Vol.110 No.-
<P><B>Abstract</B></P> <P>Poly-γ-<SMALL>D</SMALL>-glutamic acid (PGA) of anthrax is an important pathogenic factor due to its anti-phagocytic activity. Additionally, PGA has the ability to activate mouse macrophages for the secretion of cytokines through Toll-like receptor (TLR) 2. Peptidoglycan (PGN), a major bacterial cell-wall component, induces inflammatory responses in the host. We assessed whether PGA can induce maturation and cytokine expression in immature mouse dendritic cells (DCs) in the existence of muramyl dipeptide (MDP), the minimum motif of PGN with immunostimulatory activity. Stimulation of immature DCs with PGA or MDP alone augmented expression of costimulatory molecules and MHC class II proteins, which are all cell surface markers indicative of maturation. The observed effects were further enhanced by costimulation of PGA and MDP. PGA alone was sufficient to induce expression of TNF-α, IL-6, MCP-1, and MIP1-α, whereas MDP alone did not under the same conditions. Treatment with MDP enhanced PGA-induced expression of the tested inflammatory mediators; however, the synergistic effect found for PGA and MDP was not observed in TLR2- or nucleotide-binding oligomerization domain (NOD) 2–knockout DCs. Additionally, MDP augmented PGA-induced MAP kinases and NF-κB activation, which is crucial for expression of cytokines. Furthermore, MAP kinase and NF-κB inhibitors attenuated MDP enhancement of PGA-induced cytokine production. In addition, co-culture of splenocytes and PGA/MDP-matured DCs induced higher expression of IL-2 and IFN-γ compared to that of splenocytes and PGA-matured DCs. Collectively, our results suggest that PGA and MDP cooperatively induce inflammatory responses in mouse DCs through TLR2 and NOD2 via MAP kinase and NF-κB pathways, subsequently leading to lymphocyte activation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MDP enhances PGA-induced maturation of mouse DCs. </LI> <LI> MDP enhances production of inflammatory mediators by PGA. </LI> <LI> TLR2, NOD2, MAP kinase, and NF-κB signaling pathways are involved in PGA/MDP-induced cytokine production. </LI> <LI> MDP increases lymphocyte activation induced by PGA-matured DCs. </LI> </UL> </P>
Fabrication of the CuInGaSe Pellet and Characterization of the Thin Film
Jeon, Hunsoo,Lee, Ahreum,Lee, Gang-Seok,Jo, Dong-Wan,Ok, Jin-Eun,Kim, Kyoung Hwa,Yang, Min,Yi, Sam Nyung,Ahn, Hyung Soo,Cho, Chae-Ryong,Kim, Suck-Whan,Ha, Hong-Ju IOP Publishing 2011 Japanese journal of applied physics Vol.50 No.1