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EOH, Jae-Hyuk,NO, Hee Cheon,YOO, Yong-Hwan,JEONG, Ji-Young,KIM, Jong-Man,KIM, Seong-O Atomic Energy Society of Japan 2010 Journal of nuclear science and technology Vol.47 No.11
<P>For a CO<SUB>2</SUB> ingress accident into liquid sodium in a supercritical CO<SUB>2</SUB> power conversion system coupled with a sodium-cooled fast reactor, we investigated two major design issues: i) a wastage phenomenon in regard to structural damage adjacent to the leaking position, and ii) potential channel plugging due to the formation of a particulate reaction product. In order to understand the factors affecting the occurrence of these issues, two kinds of experiments were carried out: a wastage effect test and a self-plugging test. All experimental conditions were chosen to reasonably represent the normal operating conditions and realistic design parameters of the reference plant. The test results indicate the absence of wastage, which will not lead to additional tube ruptures and damage propagation. In the current experiment, the self-plugging of PCHE channels only took place under two limited conditions: i) the sodium temperature is over 500°C and ii) the equivalent diameter of the crack opening is less than 1.5 mm with a small leakage rate of far less than 1 g/s of CO<SUB>2</SUB> ingress.</P>
Eoh, Kyung Jin,Lee, So Hyun,Kim, Hee Jung,Lee, Jung-Yun,Kim, Sunghoon,Kim, Sang Wun,Kim, Young Tae,Nam, Eun Ji Elsevier 2018 Biochemical and biophysical research communication Vol.497 No.2
<P><B>Abstract</B></P> <P>MicroRNA-630 (miR-630) has been implicated in the development and progression of multiple cancers. The current study aimed to investigate the role of miR-630 in chemoresistant epithelial ovarian cancer. MiR-630 expression levels were detected in ovarian cancer cell line SKOV3 and paclitaxel-resistant SKOV3 (SKOV3-TR) via microarray and qRT-PCR. MiR-630 inhibitors and negative controls were transfected into SKOV3 and SKOV3-TR cells. Wound healing, invasion, chemosensitivity, and cell apoptosis assays were performed to determine proliferation and migration rates. Chemoresistant patient-derived xenograft (PDX) models were established and utilized to verify the effect of miR-630 on chemoresistant ovarian cancer. Inhibition of miR-630 decreased cell proliferation and enhanced the sensitivity of SKOV3-TR and SKOV3 cells to paclitaxel. In the chemosensitivity assay, we observed that the miR-630 inhibitor exhibited a synergistic effect with paclitaxel on SKOV3-TR cells. Inhibition was correlated with enhanced expression of apoptosis-related proteins. APAF-1 was predicted to be a potential target of miR-630. An <I>in vivo</I> PDX study showed that the miR-630 inhibitor sensitized chemoresistant ovarian cancer to paclitaxel. Thus, miR-630 inhibitor sensitizes chemoresistant epithelial ovarian cancer to chemotherapy by enhancing apoptosis. Our findings suggest that miR-630 might be a potential therapeutic target for chemotherapy-resistant ovarian cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the role of miR-630 in chemoresistant epithelial ovarian cancer. </LI> <LI> MiR-630 inhibition reduced proliferation and enhanced chemosensitivity <I>in vitro.</I> </LI> <LI> Inhibition was correlated with enhanced expression of apoptosis-related proteins. </LI> <LI> APAF-1 was predicted to be a potential target of miR-630. </LI> <LI> Inhibition sensitized chemoresistant ovarian cancer to paclitaxel <I>in vivo.</I> </LI> </UL> </P>
Eoh, Hyungjin,Jeon, Bo-Young,Kim, Zhiyeol,Kim, Seung-Cheol,Cho, Sang-Nae AAVLD 2010 Journal of veterinary diagnostic investigation Vol.22 No.4
<P>Brucella abortus is a bacterium of brucellosis causing abortion in cattle. The diagnosis of bovine brucellosis mainly relies on serologic tests using smooth lipopolysaccharide (S-LPS) from B. abortus. However, the usefulness of this method is limited by false-positive reactions due to cross-reaction with other Gram-negative bacteria. In the present study, the eryC gene encoding B. abortus d-erythrulose 1-phosphate dehydrogenase, which is involved in the erythritol metabolism in virulent B. abortus strain but is absent from a B. abortus vaccine strain (S19), was cloned. Recombinant EryC was expressed and purified for the evaluation as a diagnostic reagent for bovine brucellosis. Other B. abortus proteins, Omp16, PP26, and CP39 were also purified and their seroreactivities were compared. Recombinant EryC, Omp16, PP26, and PP39 were all reactive to B. abortus-positive serum. The specificity of recombinant Omp16, PP26, CP39, and EryC, were shown to be approximately 98%, whereas that of B. abortus whole cell lysates was shown to be 95%. The sensitivity of Omp16, PP26, CP39, and EryC were 10%, 51%, 64%, and 43%, respectively, whereas that of B. abortus whole cell lysates was 53%. These results suggested that B. abortus EryC would be a potential reagent for diagnosis for bovine brucellosis as a single protein antigen.</P>
Eoh, Jae-Hyuk,Park, Shane,Jeun, Gyoo-Dong,Kim, Moo-Hwan Korean Nuclear Society 2001 Nuclear Engineering and Technology Vol.33 No.2
Under severe accidents, the pressure and temperature response has an important role for the integrity of a nuclear power plant containment. The history of the pressure and temperature is characterized by the amount and state of steam/air mixture in a containment. Recently, the heat transfer rate to the structure surface is supposed to be increased by the wavy interface formed on condensate film. However, in the calculation by using CONTAIN code, the condensation heat transfer on a containment wall is calculated by assuming the smooth interface and has a tendency to be underestimated for safety. In order to obtain the best- estimate heat transfer calculation, we investigated the condensation heat transfer model in CONTAIN 1.2 code and adopted the new forced convection correlation which is considering wavy interface. By using the film tracking model in CONTAIN 1.2 code, the condensate film is treated to consider the effect of wavy interface. And also, it was carried out to investigate the effect of the different cell modelings - 5-cell and 10-cell modeling - for KNGR(Korean Next Generation Reactor) containment phenomena during a severe accident. The effect of wavy interface on condensate film appears to cause the decrease of peak temperature and pressure response . In order to obtain more adequate results, the proper cell modeling was required to consider the proper flow of steam/air mixture.
Eoh, Kyung Jin,Kim, Ji Eun,Park, Hyung Seok,Lee, Seung-Tae,Park, Ji Soo,Han, Jung Woo,Lee, Jung-Yun,Kim, Sunghoon,Kim, Sang Wun,Kim, Jae Hoon,Kim, Young Tae,Nam, Eun Ji Korean Cancer Association 2018 Cancer Research and Treatment Vol.50 No.3
<P><B>Purpose</B></P><P>Next-generation sequencing (NGS) allows simultaneous sequencing of multiple cancer susceptibility genes and may represent a more efficient and less expensive approach than sequential testing. We assessed the frequency of germline mutations in individuals with epithelial ovarian cancer (EOC), using multi-gene panels and NGS.</P><P><B>Materials and Methods</B></P><P>Patients with EOC (n=117) with/without a family history of breast or ovarian cancer were recruited consecutively, from March 2016 toDecember 2016.GermlineDNAwas sequenced using 35-gene NGS panel, in order to identify mutations. Upon the detection of a genetic alteration using the panel, results were cross-validated using direct sequencing.</P><P><B>Results</B></P><P>Thirty-eight patients (32.5%) had 39 pathogenic or likely pathogenic mutations in eight genes, including <I>BRCA1</I> (n=21), <I>BRCA2</I> (n=10), <I>BRIP1</I> (n=1), <I>CHEK2</I> (n=2), <I>MSH2</I> (n=1), <I>POLE</I> (n=1), <I>RAD51C</I> (n=2), and <I>RAD51D</I> (n=2). Among 64 patients with a family history of cancer, 27 (42.2%) had 27 pathogenic or likely pathogenic mutations, and six (9.3%) had mutations in genes other than <I>BRCA1/2</I>, such as <I>CHECK2</I>, <I>MSH2</I>, <I>POLE</I>, and <I>RAD51C</I>. Fifty-five patients (47.0%) were identified to carry only variants of uncertain significance.</P><P><B>Conclusion</B></P><P>Using the multi-gene panel test, we found that, of all patients included in our study, 32.5% had germline cancer-predisposing mutations. NGS was confirmed to substantially improve the detection rates of a wide spectrum of mutations in EOC patients compared with those obtained with the <I>BRCA1/2</I> testing alone.</P>
Eoh, Kyung Jin,Chung, Young Shin,Lee, So Hyun,Park, Sun-Ae,Kim, Hee Jung,Yang, Wookyeom,Lee, In Ok,Lee, Jung-Yun,Cho, Hanbyoul,Chay, Doo Byung,Kim, Sunghoon,Kim, Sang Wun,Kim, Jae-Hoon,Kim, Young Tae 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3
<P><B>Purpose</B></P><P>Although the use of xenograft models is increasing, few studies have compared the clinical features or outcomes of epithelial ovarian cancer (EOC) patients according to the tumorigenicity of engrafted specimens. The purpose of this study was to evaluate whether tumorigenicity was associated with the clinical features and outcomes of EOC patients. </P><P><B>Materials and Methods</B></P><P>Eighty-eight EOC patients who underwent primary or interval debulking surgery from June 2014 to December 2015 were included. Fresh tumor specimens were implanted subcutaneously on each flank of immunodeficient mice. Patient characteristics, progression-free survival (PFS), and germline mutation spectra were compared according to tumorigenicity.</P><P><B>Results</B></P><P>Xenografts were established successfully from 49 of 88 specimens. Tumorigenicity was associated with lymphovascular invasion and there was a propensity to engraft successfully with high-grade tumors. Tumors from patientswho underwent non-optimal (residual disease ≥ 1 cm) primary orinterval debulking surgery had a significantly greater propensity to achieve tumorigenicity than those who received optimal surgery. In addition, patients whose tumors became engrafted seemed to have a shorter PFS and more frequent germline mutations than patients whose tumors failed to engraft. Tumorigenicity was a significant factor for predicting PFS with advanced International Federation of Gynecology and Obstetrics stage and high-grade cancers.</P><P><B>Conclusion</B></P><P>sTumorigenicity in a xenograft model was a strong prognostic factor and was associated with more aggressive tumors in EOC patients. Xenograft models can be useful as a preclinical tool to predict prognosis and could be applied to further pharmacologic and genomic studies on personalized treatments.</P>