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- 저자 : Edgardo E. Tulin (검색결과 1 건)
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Cytokine-Mimetic Properties of Some Philippine Food and Medicinal Plants
Edgardo E. Tulin,Zenaida T. Ecleo 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.2
This study evaluated Philippine indigenous plants for cytokine-mimetic properties and partially characterizedcandidate mimetics for their stability as well as their sensitivity to variations in temperature and pH. Forty-five plant extractpreparations were tested for their ability to stimulate proliferation of mouse spleen cells and bone marrow cells. Temperatureand pH effects were determined by subjecting the extracts to different temperature and pH levels and measuring their resid-ual proliferative activities. Results revealed that 24 and 14 extracts were able to stimulate proliferation of bone marrow cellsand spleen cells, respectively. Extracts from yam (Dioscorea alata L.) (Dioscoreaceae) roots and leaves, taro (Colocasia es-culenta L.) (Araceae) roots, and buyo (Piper betleL.) (Piperaceae) leaf were among the extracts that strongly stimulated pro-liferation of both bone marrow cells and splenocytes, significantly increasing cell concentrations. Heating the extracts beyond40°C markedly reduced their proliferative ability, while a pH level below 4.0 and above 8.0 also significantly decreased theextracts’ activity. Three protein-mimetics with sizes of 26 kDa, 35 kDa, and 50 kDa were isolated from buyo leaf, yam root,and taro root extracts, respectively. All three molecules are biologically active and stimulate a dose-dependent proliferativeresponse.
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