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Biswaranjan Behera,Shashikanta Behera,Shasmita,Debasish Mohapatra,Durga Prasad Barik,Soumendra Kumar Naik 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.4
Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal commu- nities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low tem- perature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 °C; thus, they could be useful for transfer and exchange of P. foetida germplasm.
Model-Based Test-Suite Minimization using Modified Condition/Decision Coverage (MC/DC)
Ajay Kumar Jena,Santosh Kumar Swain,Durga Prasad Mohapatra 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.5
Testing is very expensive for high-assurance software, like commercial aircraft systems, weapon research, weather forecast, earthquake forecast, and software used for safety critical system. A small and simple flaw in the end product can be enough for destroying the entire effort of the developer with a huge unrecoverable damage to the society. For this reason, Federal Aviation Administration’s requirement is that, the test-suites should be comprises of Modified Condition/Decision Coverage (MC/DC) adequate. By using logic coverage criteria lots of flaws can be removed for safety critical software. MC/DC was proposed by NASA, and had been widely accepted in the field of testing. MC/DC is an effective verification technique, and helps to uncover safety faults. It is a challenge to minimize the number of test-suites when there is a partial change in the software. This can be achieved by using models. Unified Modeling Language (UML) not only helps to design software but also plays a vital role in detecting the faults early phase of design and in minimizing the test-suite. Existing test-suite minimization techniques investigated by different researchers may not be effective in minimizing MC/DC-adequate test-suites because they do not consider the complexity of the present software. A new approach for test-suite minimization is presented in this work, using dissimilarity matrix, which can be well fitted with MC/DC. We also present the results generated out of a case study of the test-suite minimization.