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Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)
Rongjun Fang,Dongqing Hu,Yinghua Zhang,Long Li,Weiguo Zhao,Li Liu,Jialin Cheng,Jinliang Qi,Yonghua Yang 한국유전학회 2011 Genes & Genomics Vol.33 No.2
A full-length cDNA sequence encoding CaM from mulberry,which we designated MCaM-3 (GenBank accession No:GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.