A New Approach Using the SYBR Green-Based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage

Yong Ju Jin(Yong Ju Jin),Dawon Jo(Dawon Jo),Soon-Wo Kwon(Soon-Wo Kwon),Samnyu Jee(Samnyu Jee),Jeong-Seon Kim(Jeong-Seon Kim),Jegadeesh Raman(Jegadeesh Raman ),Soo-Jin Kim(Soo-Jin Kim) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferumspecific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue.

Enhanced Expression of TREK-1 Is Related with Chronic Constriction Injury of Neuropathic Pain Mouse Model in Dorsal Root Ganglion

( Hyo Jo Han ),( Seung Wook Lee ),( Gyu Tae Kim ),( Eun Jin Kim ),( Byeonghun Kwon ),( Dawon Kang ),( Hyun Jeong Kim ),( Kwang Suk Seo ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.3

Neuropathic pain is a complex state showing increased pain response with dysfunctional inhibitory neurotransmission. The TREK family, one of the two pore domain K+ (K2P) channel subgroups were focused among various mechanisms of neuropathic pain. These channels influence neuronal excitability and are thought to be related in mechano/thermosensation. However, only a little is known about the expression and role of TREK-1 and TREK-2, in neuropathic pain. It is performed to know whether TREK-1 and/ or 2 are positively related in dorsal root ganglion (DRG) of a mouse neuropathic pain model, the chronic constriction injury (CCI) model. Following this purpose, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and western blot analyses were performed using mouse DRG of CCI model and compared to the sham surgery group. Immunofluorescence staining of isolectin-B4 (IB4) and TREK were performed. Electrophysiological recordings of single channel currents were analyzed to obtain the information about the channel. Interactions with known TREK activators were tested to confirm the expression. While both TREK-1 and TREK-2 mRNA were significantly overexpressed in DRG of CCI mice, only TREK-1 showed significant increase (~9 fold) in western blot analysis. The TREK-1-like channel recorded in DRG neurons of the CCI mouse showed similar current-voltage relationship and conductance to TREK-1. It was easily activated by low pH solution (pH 6.3), negative pressure, and riluzole. Immunofluorescence images showed the expression of TREK-1 was stronger compared to TREK-2 on IB4 positive neurons.

Enhanced Expression of TREK-1 Is Related with Chronic Constriction Injury of Neuropathic Pain Mouse Model in Dorsal Root Ganglion

Han, Hyo Jo,Lee, Seung Wook,Kim, Gyu-Tae,Kim, Eun-Jin,Kwon, Byeonghun,Kang, Dawon,Kim, Hyun Jeong,Seo, Kwang-Suk The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.3

Neuropathic pain is a complex state showing increased pain response with dysfunctional inhibitory neurotransmission. The TREK family, one of the two pore domain $K^+$ (K2P) channel subgroups were focused among various mechanisms of neuropathic pain. These channels influence neuronal excitability and are thought to be related in mechano/thermosensation. However, only a little is known about the expression and role of TREK-1 and TREK-2, in neuropathic pain. It is performed to know whether TREK-1 and/or 2 are positively related in dorsal root ganglion (DRG) of a mouse neuropathic pain model, the chronic constriction injury (CCI) model. Following this purpose, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and western blot analyses were performed using mouse DRG of CCI model and compared to the sham surgery group. Immunofluorescence staining of isolectin-B4 (IB4) and TREK were performed. Electrophysiological recordings of single channel currents were analyzed to obtain the information about the channel. Interactions with known TREK activators were tested to confirm the expression. While both TREK-1 and TREK-2 mRNA were significantly overexpressed in DRG of CCI mice, only TREK-1 showed significant increase (~9 fold) in western blot analysis. The TREK-1-like channel recorded in DRG neurons of the CCI mouse showed similar current-voltage relationship and conductance to TREK-1. It was easily activated by low pH solution (pH 6.3), negative pressure, and riluzole. Immunofluorescence images showed the expression of TREK-1 was stronger compared to TREK-2 on IB4 positive neurons. These results suggest that modulation of the TREK-1 channel may have beneficial analgesic effects in neuropathic pain patients.

Transcriptional Response of Pectobacterium carotovorum to Cinnamaldehyde Treatment

( Jihye Jung ),( Dawon Jo ),( Soo-jin Kim ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.3

Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown gall and bacterial canker diseases, respectively has been documented, indepth studies into cinnamaldehyde’s broader influence on plant pathogenic bacteria are relatively unexplored. Particularly, Pectobacterium spp., gram-negative soil-borne pathogens, notoriously cause soft rot damage across a spectrum of plant families, emphasizing the urgency for effective treatments. Our investigation established that the Minimum Inhibitory Concentrations (MICs) of cinnamaldehyde against strains P. odoriferum JK2, P. carotovorum BP201601, and P. versatile MYP201603 were 250 μg/ml, 125 μg/ml, and 125 μg/ml, respectively. Concurrently, their Minimum Bactericidal Concentrations (MBCs) were found to be 500 μg/ml, 250 μg/ml, and 500 μg/ml, respectively. Using RNA-sequencing analysis, we identified 1,907 differentially expressed genes in P. carotovorum BP201601 treated with 500 μg/ml cinnamaldehyde. Notably, our results indicate that cinnamaldehyde upregulated nitrate reductase pathways while downregulating the citrate cycle, suggesting a potential disruption in the aerobic respiration system of P. carotovorum during cinnamaldehyde exposure. This study serves as a pioneering exploration of the transcriptional response of P. carotovorum to cinnamaldehyde, providing insights into the bactericidal mechanisms employed by cinnamaldehyde against this bacterium.

Spinal Cord Injury Markedly Altered Protein Expression Patterns in the Affected Rat Urinary Bladder during Healing Stages

Lee, Ji-Young,Kim, Bong Jo,Sim, Gyujin,Kim, Gyu-Tae,Kang, Dawon,Jung, Jae Hun,Hwa, Jeong Seok,Kwak, Yeon Ju,Choi, Yeon Jin,Park, Young Sook,Han, Jaehee,Lee, Cheol Soon,Kang, Kee Ryeon The Korean Academy of Medical Sciences 2011 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.26 No.6

<P>The influence of spinal cord injury (SCI) on protein expression in the rat urinary bladder was assessed by proteomic analysis at different time intervals post-injury. After contusion SCI between T9 and T10, bladder tissues were processed by 2-DE and MALDI-TOF/MS at 6 hr to 28 days after SCI to identify proteins involved in the healing process of SCI-induced neurogenic bladder. Approximately 1,000 spots from the bladder of SCI and sham groups were visualized and identified. At one day after SCI, the expression levels of three protein were increased, and seven spots were down-regulated, including heat shock protein 27 (Hsp27) and heat shock protein 20 (Hsp20). Fifteen spots such as S100-A11 were differentially expressed seven days post-injury, and seven proteins including transgelin had altered expression patterns 28 days after injury. Of the proteins with altered expression levels, transgelin, S100-A11, Hsp27 and Hsp20 were continuously and variably expressed throughout the entire post-SCI recovery of the bladder. The identified proteins at each time point belong to eight functional categories. The altered expression patterns identified by 2-DE of transgelin and S100-A11 were verified by Western blot. Transgelin and protein S100-A11 may be candidates for protein biomarkers in the bladder healing process after SCI.</P>

신입병사에서 충동성이 자살사고에 미치는 영향

안인영(In-Young Ahn),이다원(Dawon Lee),박철수(Chul-Soo Park),김봉조(Bong-Jo Kim),이철순(Cheol-Soon Lee),차보석(Boseok Cha),이소진(So-Jin Lee),서지영(Ji-Yeong Seo),최재원(Jae-Won Choi),이동윤(Dongyun Lee) 대한생물치료정신의학회 2016 생물치료정신의학 Vol.22 No.2

Objectives:Diverse psychiatric symptoms and individual traits comprise risk factors of suicide ideation. The aim of this study was to investigate whether depressive symptoms and stress-related symptoms mediate between impulsivity and suicidal ideation. Methods:A total of 445 participants completed the Barratt Impulsiveness Scale-11-Revised Korean version(KBIS-11), Scale for Suicide ideation(SSI), Center for Epidemiological Studies-Depression Scale(CES-D), and Impact Event Scale-Revised Korean version(IES-R-K) before basic military training. Structural Equation Model(SEM) was used to determine integrated relationship between impulsivity, suicide ideation, depressive symptoms and stress-related symptoms. Results:SEM showed good model fit. The direct effect was 0.107(p<0.05) between impulsivity and suicide ideation; and the indirect effect was 0.199(p<0.01) between impulsivity and suicidal ideation through depressive symptoms and stress-related symptoms. Conclusion:The present study revealed a significant mediating effect between impulsivity and suicidal ideation through depressive symptoms and stress-related symptoms. The findings indicated that comprehensive assessment of impulsivity, depressive symptoms and stress-related symptoms are needed for evaluation of suicidal ideation.

심층학습을 이용한 국내 및 해외 국가의 외래어류 위해성 평가

서상익(Sang-Ik Suh),조경철(Gyeong Cheol Jo),남건우(Gun-Woo Nam),김다원(Dawon Kim),안상준(Sang Jun Ahn),기서진(Seo Jin Ki) 대한환경공학회 2022 대한환경공학회 학술발표논문집 Vol.2022 No.11

Reduced graphene oxide-assisted crystallization of perovskite via solution-process for efficient and stable planar solar cells with module-scales

Yeo, Jun-Seok,Lee, Cheol-Ho,Jang, Dawon,Lee, Sungho,Jo, Seung Mu,Joh, Han-Ik,Kim, Dong-Yu Elsevier 2016 Nano energy Vol.30 No.-

<P><B>Abstract</B></P> <P>Organometal trihalide perovskite solar cells (PeSCs) have recently opened a new era for photovoltaics power sources via the tremendous increase in power conversion efficiency (PCE) in the past five years. The next achievement will occur when scalable and processable PeSCs are realized because of a fundamental understanding of the interfacial characteristics and perovskite crystallization in PeSCs. Here, we report solution-processed planar PeSCs with well-tailored functional graphene, successfully demonstrating excellent module PCEs of 10.0% and 8.1% for rigid and flexible substrates, respectively, with active-area of 10cm<SUP>2</SUP>. Systematic investigations reveal that molecular-doped reduced graphene oxide with fluorine atoms (MFGO) exhibits fast charge-extraction ability and well-aligned energetic interface characteristic due to its intrinsic structure, and MFGO promotes perovskite crystallization and orientation with minimized stoichiometric defects. The solution-processable graphene can function not only as an efficient and stable interlayer but also as an inducer of the crystallization of the perovskite layer in simplified device architectures without a complex process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Interfacial characteristics and crystallinity of active-layer in perovskite solar cells (PeSCs) are tailored by fluorinated reduced graphene oxide (MFGO). </LI> <LI> Comprehensive investigation reveals the multifunctional roles of MFGO as the interfacial layer and crystallinity inducer. </LI> <LI> MFGO-based modules with a 10cm<SUP>2</SUP> active-area exhibit promising PCEs of 10.0% and 8.1% for rigid and flexible substrates, respectively. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>