Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

Ghosh, M.K.,Das, B.K.,Das, C.,Mishra, A.K.,Mukherjee, P.K.,Urs, S.Raje Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.1

Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year, The nitrate reductase (EC No. 1.6.6.1) activity (NRA, $\mu$mol N $O_2$- $h^{-1}$ $g^{-1}$ fr, wt.), total soluble protein (mg $g^{-1}$ fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area ($\textrm{cm}^2$), specific leaf weight (g c $m^{-2}$ ), total soluble sugar (mg $g^{-1}$ fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, $\mu$㏖ $m^{2}$ $s^{-1}$ ) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 $\mu$㏖ N $O_2$- $h^{-l}$ $g^{-1}$ fr, wt.), total soluble protein (39.63mg $g^{-1}$ fr, wt.), NPR(16.66 $\mu$㏖ $m^{-2}$ $s^{-1}$ ), total soluble sugar (48.44 mg $g^{-1}$ fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA af unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.t.

A Point Mutation at the C-Terminal Half of the Repressor of Temperate Mycobacteriophage L1 Affects Its Binding to the Operator DNA

Ganguly, Tridib,Chattoraj, Partho,Das, Malabika,Chanda, Palas K.,Mandal, Nitai.C.,Lee, Chia Y.,Sau, Subrata Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.6

The wild-type repressor CI of temperate mycobacteriophage L1 and the temperature-sensitive (ts) repressor CIts391 of a mutant L1 phage, L1cIts391, have been separately overexpressed in E. coli. Both these repressors were observed to specifically bind with the same cognate operator DNA. The operator-binding activity of CIts391 was shown to differ significantly than that of the CI at 32 to $42^{\circ}C$. While 40-95% operator-binding activity was shown to be retained at 35 to $42^{\circ}C$ in CI, more than 75% operator-binding activity was lost in CIts391 at 35 to $38^{\circ}C$, although the latter showed only 10% less binding compared to that of the former at $32^{\circ}C$. The CIts391 showed almost no binding at $42^{\circ}C$. An in vivo study showed that the CI repressor inhibited the growth of a clear plaque former mutant of the L1 phage more strongly than that of the CIts391 repressor at both 32 and $42^{\circ}C$. The half-life of the CIts391-operator complex was found to be about 8 times less than that of the CI-operator complex at $32^{\circ}C$. Interestingly, the repressor-operator complexes preformed at $0^{\circ}C$ have shown varying degrees of resistance to dissociation at the temperatures which inhibit the formation of these complexes are inhibited. The CI repressor, but not that of CIts391, regains most of the DNA-binding activity on cooling to $32^{\circ}C$ after preincubation at 42 to $52^{\circ}C$. All these data suggest that the 131st proline residue at the C-terminal half of CI, which changed to leucine in the CIts391, plays a crucial role in binding the L1 repressor to the cognate operator DNA, although the helix-turn-helix DNA-binding motif of the L1 repressor is located at its N-terminal end.

A Point Mutation at the C-Terminal Half of the Repressor of Temperate Mycobacteriophage L1 Affects Its Binding to the Operator DNA

( Tridib Ganguly ),( Partho Chattoraj ),( Malabika Das ),( Palas K. Chanda ),( Nitai. C. Mandal ),( Chia Y. Lee ),( Subrata Sau ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.6

The wild-type repressor CI of temperate mycobacteriophage Ll and the temperature-sensitive (ts) repressor CIts391 of a mutant Ll phage, LlcIts391, have been separately overexpressed in E. coli. Both these repressors were observed to specifically bind with the same cognate operator DNA. The operator-binding activity of CIts391 was shown to differ significantly than that of the CI at 32 to 42C. While 40-95% operator-binding activity was shown to be retained at 35 to 42C in CI, more than 75% operator-binding activity was lost in CIts391 at 35 to 38℃, although the latter showed only 10% less binding compared to that of the former at 32℃. The CIts391 showed almost no binding at 42℃. An in vivo study showed that the CI repressor inhibited the growth of a clear plaque former mutant of the L1 phage more strongly than that of the CIts391 repressor at both 32 and 42℃. The half-life of the CIts391-operator complex was found to be about 8 times less than that of the CI-operator complex at 32C. Interestingly, the repressor-operator complexes preformed at 0C have shown varying degrees of resistance to dissociation at the temperatures which inhibit the formation of these complexes are inhibited. The CI repressor, but not that of CIts391, regains most of the DNA-binding activity on cooling to 32C after preincubation at 42 to 52C. All these data suggest that the 131 proline residue at the C-terminal half of CI, which changed to leucine in the CIts391, plays a crucial role in binding the L1 repressor to the cognate operator DNA, although the helix-turn-helix DNA-binding motif of the LI repressor is located at its N-terminal end.

Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

( M. K. Ghosh ),( B. K. Das ),( C. Das ),( A. K. Mishra ),( P. K. Mukherjee ),( S. Raje Urs ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1

Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, μmol NO2-h-1 g-1 fr, wt.), total soluble protein (mg g-1 fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area (㎠), specific leaf weight (g ㎝-2), total soluble sugar (mg g-1 fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, μ㏖ m-2 g-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 μ㏖ NO2-h-1g-1 fr, wt.), total soluble protein (39.63 mg g-1 fr, wt.), NPR (16.66 μ㏖ m-2 S-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.

Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

M. K. Ghosh,B. K. Das,C. Das,A. K. Mishra,P. K. Mukherjee,S. Raje Urs 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.1

Ten improved mulberry varieties (V1, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, mmol NO2 - h-1g-1 fr. wt.), total soluble protein (mg g-1 fr. wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture%, unit leaf area (cm2), specific leaf weight (g cm-2), total soluble sugar (mg g-1 fr. wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, mmol m-2 s-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, having higher NRA (13.25 mmol NO2 - h-1g-1 fr. wt.), total soluble protein (39.63 mg g-1 fr. wt.), NPR (16.66 mmol m-2 s-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.

Proteomic changes induced by histone demethylase JMJD3 in TNF alpha-treated human monocytic (THP-1) cells

Das, A.,Das, N.D.,Jung, K.H.,Park, J.H.,Lee, H.T.,Han, D.,Choi, M.R.,Kang, S.C.,Chai, Y.G. Pergamon Press 2013 Molecular immunology Vol.56 No.1

JMJD3, a Jumonji C family histone demethylase, plays an important role in the regulation of inflammation induced by the transcription factor nuclear factor-kappa B (NF-κB) in response to various stimuli. JMJD3 is a histone-3 lysine-27 trimethylation (H3K27me3) demethylase, a histone mark associated with transcriptional repression and activation of a diverse set of genes. The present study assessed stable JMJD3 knockdown (KD)-dependent proteomic profiling in human leukemia monocyte (THP-1) cells to analyze the JMJD3-mediated differential changes of marker expression in inflammatory cells. To analyze the protein expression profile of tumor necrosis factor-alpha (TNF-α)-stimulated JMJD3-kd THP-1 cells, we employed matrix-assisted-laser-desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Additionally, Ingenuity Pathways Analysis (IPA) was applied to establish the molecular networks. A comparative proteomic profile was determined in TNF-α-treated both of JMJD3-kd THP-1 cells and THP-1 scrambled (sc) cells. The expression of tripartite motif protein (TRIM5), glutathione peroxidase (GPx), glia maturation factor-γ (GMFG), caspase recruitment domain family, member 14 (CARMA2), and dUTP pyrophosphatase were significantly down-regulated, whereas heat shock protein beta-1 (HspB1) and prohibition were significantly up-regulated in JMJD3-kd THP-1 cells. The molecular and signaling networks of the differentially expressed proteins in JMJD3-kd THP-1 cells were determined by IPA. The molecular network signatures and functional proteomics obtained in this study may facilitate the suppression of different key inflammatory regulators through JMJD3-attenuation, which would be crucial to evaluate potential therapeutic targets and to elucidate the molecular mechanism of JMJD3-kd dependent effects in THP-1 cells.

Surface and interface studies of RF sputtered HfO2 thin films with working pressure and gas flow ratio

Das, K. C.,Ghosh, S. P.,Tripathy, N.,Bose, G.,Ashok, A.,Pal, P.,Kim, D. H.,Lee, T. I.,Myoung, J. M.,Kar, J. P. Springer Science + Business Media 2015 Journal of materials science Materials in electron Vol.26 No.8

Effect of Foliar Treatment of KCl on Chlorophyll, Total Sugars, Soluble Protein, In Vivo Nitrate Reductase Activity and Leaf Yield in Mulberry(Morus alba L. CV.S1)

C. Das,M. K. Ghosh,B. K. Das,A. K. Misra,P. K. Mukherjee,S. Raje Urs 한국잠사학회 2003 International Journal of Industrial Entomology Vol.7 No.1

Influence of Level of Dietary Inorganic and Organic Copper and Energy Level on the Performance and Nutrient Utilization of Broiler Chickens

Das, T.K.,Mondal, M.K.,Biswas, P.,Bairagi, B.,Samanta, C.C. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1

An experiment was conducted to determine the influence of dietary inorganic (copper sulfate) and organic (copper proteinate) forms of copper and energy level on performance and nutrient utilization of broiler chickens. Two hundred day-old commercial Vencobb broiler chicks were purchased and randomly distributed to 20 cages of 10 birds each. These replicates were randomly assigned to one of five treatments in a (($2{\times}2$)+1) factorial arrangement. These two factors were sources of Cu ($CuSO_{4}$ vs. Cuproteinate) and dose of Cu supplements (200 mg and 400 mg/kg dietary dry matter) and the control (no supplemental Cu). After the starter period (up to 3 weeks), from d 22 onwards another factor i.e. energy at two levels (2,900 vs. 2,920 kcal/kg diet) was introduced with the previous factorial arrangements by subdividing each replicate into two equal parts, for two energy levels, without disturbing the dose and source of Cu supplement. Cu-salt supplementation linearly increased (p<0.01) live weight (LW), live weight gain (LWG) and feed conversion ratio (FCR) at 3 weeks, whereas cumulative feed intake (CFI) was unaffected (p>0.05). LWG and FCR were higher (p<0.01) in Cu-proteinate supplemented birds compared to $CuSO_{4}$ supplementation. A linear dose response (p<0.01) of Cu was found for the performance of broiler chickens. Birds having a higher energy level in the finisher stage increased (p<0.01) LWG and FCR. Cumulative feed intake was similar (p>0.05) across the groups up to the 5th week. Cu-proteinate increased performance of broiler chickens compared to $CuSO_{4}$. Dose of supplemental Cu-salt irrespective of source showed a linear response (p<0.01) for performance. Supplementation of Cu-proteinate increased metabolizability of DM (p<0.01), NFE (p<0.05), total carbohydrate (p<0.01) and OM (p<0.01) at the starter period. Increased dose of Cu-salt linearly increased (p<0.01) metabolizability of DM, CP, CF, NFE and OM. Higher energy level in the diet improved DM (p<0.05), EE (p<0.01), NFE (p = 0.01), total carbohydrate (p<0.01) and OM (p<0.01) metabolizability. Cu-proteinate supplementation showed better nutrient utilization compared to CuSO4. Dose of Cu linearly increased DM, CP, EE, NFE, total carbohydrate and OM metabolizability. CF metabolizability was unaffected (p>0.05) among the treatments. In conclusion, dietary supplementation of Cu-salt more than the requirement may improve performance and nutrient utilization in broiler chickens even with a high energy finisher diet. Cu-proteinate showed better performance and nutrient utilization compared to $CuSO_{4}$.

Lyophilized somatic cells direct embryonic development after whole cell intracytoplasmic injection into pig oocytes

Das, Z.C.,Gupta, M.K.,Uhm, S.J.,Lee, H.T. Academic Press 2010 Cryobiology Vol.61 No.2

The study investigated the feasibility of lyophilization for long-term preservation of somatic cells and embryonic development after whole cell intracytoplasmic injection (WCICI) into enucleated pig oocyte. Confluent cultured porcine fetal fibroblast (pFF) cells were lyophilized and stored at 4<SUP>o</SUP>C for at least 6months. Results showed that compared to non-lyophilized control cells, lyophilized cells had drastically reduced cellular viability (P<0.01). WCICI of reconstituted lyophilized cells could support complete embryonic development. However, the rates of cleavage (64.7+/-2.7 vs. 43.5+/-4.7%) and blastocyst formation (18.2+/-0.6 vs. 10.2+/-1.6%) were lower than that of control (P<0.05). Total nuclei number per blastocyst (30.4+/-4.5 vs. 25.2+/-4.7) and intensity of acetylation at histone H3 (AcH3) protein (55.9+/-3.5 vs. 53.3+/-3.8) did not differ (P>0.05). The development ability of embryos, produced from lyophilized somatic cells, was further increased (19.5+/-2.4 vs. 10.2+/-1.6%; P<0.05) by treatment with trichostatin A (TSA) for 24h post-activation. These TSA-treated embryos also had AcH3 level comparable with in vitro fertilized embryos (63.1+/-3.2 vs. 69.9+/-1.3). In conclusion, our results suggest that lyophilized somatic cells can direct embryonic development up to blastocyst stage after WCICI into pig oocytes. Treatment of embryos, produced from lyophilized somatic cells, with TSA can further increase their in vitro developmental potential.