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폴리비닐클로라이드 해양광생물반응기와 고밀도 폴리에틸렌 해양광생물반응기에서 미세조류, Tetraselmis sp. KCTC12236BP의 생산성 비교
정성균(Seung-Gyun Jung),김수권(Su-Kwon Kim),변문섭(Moon-Sup Bun),조용희(Yonghee Cho),신동우(Dong-Woo Shin),김지훈(Z-Hun Kim),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2016 한국해양바이오학회지 Vol.8 No.1
It is important to design photobioreactor by cheap material for economical microalgal biomass production. In this study, two types of marine photobioreactors (MPBR), made by either polyvinyl chloride (MPBR-PVC) or high density poly ethylene (MPBR-HDPE), are used and performance of these were compared. Tetraselmis sp. KCTC 12236BP is a green marine alga that isolated from Ganghwa Island, Korea, and the strain was used for marine cultivations using MPBR-PVC and MPBR-HDPE. The cultivations were performed three times in the spring season of 2012 using MPBR-PVC and of 2013 using MPBR-HDPE in the coastal area of Young Heung Island. As the results, MPBR-PVC shows higher biomass productivities than MPBR-HDPE, due to its high light transmittance. In the cultivations using MPBR-PVC, the average sea water temperature was 11.5°C during the first experiment and 16.5°C during the second and third experiments. Average light intensities during three times for experiments were 407.5, 268.1 and 273.0 μ·E·m<SUP>-2</SUP>·s<SUP>-1</SUP>, respectively. The maximum fresh cell weight and average biomass productivity were 1.2 g·L<SUP>-1</SUP> and 0.12 g·L<SUP>-1</SUP>·day<SUP>-1</SUP>. These results showed that Tetraselmis sp. KCTC12236BP were adapted well with the environmental conditions from ocean, and grow in the MPBR-PVC and MPBR-HDPE.
부유형 해양 광생물반응기의 선택적 투과막의 술폰화 반응을 통한 Biofouling 억제 및 미세조류 생산성 향상
김광민(Kwangmin Kim),이윤우(Yunwoo Lee),김지훈(Z-Hun Kim),박한울(Hanwool Park),정인재(Injae Jung),박재훈(Jaehoon Park),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2017 한국해양바이오학회지 Vol.9 No.1
The purpose of this study was to inhibit biofouling on a selectively permeable membrane (SPM) and increase biomass productivity in marine photobioreactors (PBRs) for microalgal cultivation by chemical treatment. Surfaces of a SPM, composed of polyethylene terephthalate (PET), was sulfonated to decrease hydrophobicity through attaching negatively charged sulfonic groups. Reaction time of sulfonation was varied from 0 min to 60 min. As the reaction time increased, the water contact angle value of SPM surface was decreased from 75.5o to 44.5o, indicating decrease of surface hydrophobicity. Furthermore, the water permeability of sulfonated SPM was increased from 5.42 mL/㎡/s to 10.58 mL/㎡/s, which reflects higher nutrients transfer rates through the membranes, due to decreased hydrophobicity. When cultivating Tetraselmis sp. using 100-mL floating PBRs with sulfonated SPMs, biomass productivity was improved by 34% compared with the control group (non-reacted SPMs). In addition, scanning electron microscopic observation of SPMs used for cultivation clearly revealed lower degree of cell attachment on the sulfonated SPMs. These results suggest that sulfornation of a PET SPM could improve microalgal biomass productivity by increasing nutrients transfer rates and inhibiting biofouling by algal cells.