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- 저자 : Chaeseung LIM (검색결과 5 건)
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Magnetic Separation of Malaria-Infected Red Blood Cells in Various Developmental Stages
Nam, Jeonghun,Huang, Hui,Lim, Hyunjung,Lim, Chaeseung,Shin, Sehyun American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.15
<P>Malaria is a serious disease that threatens the public health, especially in developing countries. Various methods have been developed to separate malaria-infected red blood cells (i-RBCs) from blood samples for clinical diagnosis and biological and epidemiological research. In this study, we propose a simple and label-free method for separating not only late-stage but also early-stage i-RBCs on the basis of their paramagnetic characteristics due to the malaria byproduct, hemozoin, by using a magnetic field gradient. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with a ferromagnetic wire fixed on a glass slide. To evaluate the performance of the microfluidic device containing the ferromagnetic wire, lateral displacement of NaNO<SUB>2</SUB>-treated RBCs, which also have paramagnetic characteristics, was observed at various flow rates. The results showed excellent agreement with theoretically predicted values. The same device was applied to separate i-RBCs. Late-stage i-RBCs (trophozoites and schizonts), which contain optically visible black dots, were separated with a recovery rate of approximately 98.3%. In addition, using an optimal flow rate, early-stage (ring-stage) i-RBCs, which had been difficult to separate because of their low paramagnetic characteristics, were successfully separated with a recovery rate of 73%. The present technique, using permanent magnets and ferromagnetic wire in a microchannel, can effectively separate i-RBCs in various developmental stages so that it could provide a potential tool for studying the invasion mechanism of the malarial parasite, as well as performing antimalarial drug assays.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-15/ac4012057/production/images/medium/ac-2013-012057_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac4012057'>ACS Electronic Supporting Info</A></P>
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박기학 ( Kihak Park ),이호식 ( Hosik Lee ),하준수 ( Junsu Ha ),김극태 ( Keugtae Kim ),임채승 ( Chaeseung Lim ) 한국물환경학회 2021 한국물환경학회지 Vol.37 No.2
It is generally known that a wastewater treatment plant (WWTP) consumes immense energy even if it can produce energy. With an aim to increase the energy independence rate of WWTP from 3.5% in 2010 to 50% in 2030, the Korean government has invested enormous research funds. In this study, cost-effective operating alternatives were investigated by analyzing the energy efficiency and economic feasibility for biogas and power generation using new and renewable energy. Based on the US EPA Energy Conservation Measures and Korea ESCO projects, energy production and independence rate were also analyzed. The main energy consumption equipment in WWTP is the blower for aeration, discharge pump for effluent, and pump for influent. Considering the processes of WWTP, the specific energy consumption rate of the process using media and MBR was the lowest (0.549 kWh/m<sup>3</sup>) and the highest (1.427 kWh/m<sup>3</sup>), respectively. Energy-saving by enhancing anaerobic digester efficiency was turned out to be efficient when in conjunction with stable wastewater treatment. The result of economic analysis (B/C ratio) was 2.5 for digestive gas power generation, 0.86 for small hydropower, 0.49 for solar energy, and 0.15 for wind energy, respectively. Furthermore, it was observed that the energy independence rate could be enhanced by installing energy production facilities such as solar and small hydropower and reducing energy consumption via the replacement of high-efficiency operating.
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