Evaluation on the Express Enterprise’s Service Quality of Customers Perception Based on SPSS and Unascertained Measure Model

Guo Caiyun,Liu Zhiqiang,Zhang Congyan 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.3

The rapid development of E-commerce has prompted increasing demand for express services. Improving service quality from the customers perception has become an important way for the private express enterprises to face the fierce market competition. This paper summarizes the customers perception service quality theory and the express enterprises’ service quality evaluation methods, and establishes an index system of private express enterprises’ service quality evaluation from the perspective of the customers perception. The SPSS software is used to detect the feasibility of the established evaluation index system. The information entropy theory is applied to calculate the index weight value, and the evaluation model of private express enterprises’ service quality is established by combining the entropy weight with the unascertained measure method. Finally, this paper carries out the empirical analysis based on the JY express enterprise’s service quality,and puts forward the relevant countermeasures and suggestions. The purpose of the paper is to provide a new scientific method for the service quality evaluation of the private express enterprises.

Benzidine Induces Epithelial-Mesenchymal Transition of Human Bladder Cancer Cells through Activation of ERK5 Pathway

Sun, Xin,Zhang, Tao,Deng, Qifei,Zhou, Qirui,Sun, Xianchao,Li, Enlai,Yu, Dexin,Zhong, Caiyun Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.3

Benzidine, a known carcinogen, is closely associated with the development of bladder cancer (BC). Epithelial-mesenchymal transition (EMT) is a critical pathophysiological process in BC progression. The underlying molecular mechanisms of mitogen-activated protein kinase (MAPK) pathway, especially extracellular regulated protein kinases 5 (ERK5), in regulating benzidine-induced EMT remains unclarified. Hence, two human bladder cell lines, T24 and EJ, were utilized in our study. Briefly, cell migration was assessed by wound healing assay, and cell invasion was determined by Transwell assay. Quantitative PCR and western blot were utilized to determine both gene expressions as well as protein levels of EMT and MAPK, respectively. Small interfering RNA (siRNA) was transfected to further determine ERK5 function. As a result, the migration and invasion abilities were enhanced, epithelial marker expression was decreased while mesenchymal marker expression was increased in human BC cell lines. Meanwhile, benzidine administration led to activation of ERK5 and activator protein 1 (AP-1) proteins, without effective stimulation of the Jun N-terminal kinase (JNK) or p38 pathways. Moreover, Benzidine-induced EMT and ERK5 activation were completely suppressed by XMD8-92 and siRNAs specific to ERK5. Of note, ERK1/2 was activated in benzidine-treated T24 cells, while benzidine-induced EMT could not be reversed by U0126, an ERK1/2 inhibitor, as indicated by further study. Collectively, our findings revealed that ERK5-mediated EMT was critically involved in benzidine-correlated BC progression, indicating the therapeutic significance of ERK5 in benzidine-related BC.

An Improvement Detection Technology of APF based on the Digital Sliding Filter

Man Chuntao,Zhang Bo,Zhang Caiyun,Yu Shanyu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.4

Genome-wide analysis of the GRAS gene family exhibited expansion model and functional differentiation in sea buckthorn (Hippophae rhamnoides L.)

Yu Liyang,Zhang Guoyun,Lyu Zhongrui,He Caiyun,Zhang Jianguo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

Key message Genome-wide identification of the sea buckthorn GRAS genes and their transcription profiles in dif- ferent organizations. Abstract GRAS proteins comprise a large family of transcription factors that experienced extensive replication, and play important roles in many aspects of growth regulatory and environmental signals. Sea buckthorn is a unique and valuable plant which has tremendous value for medical researching, ecological protection, and providing daily necessities. We iden- tified, characterized, and distinguished duplication models of GRAS transcription factors in sea buckthorn. A systematic whole-genome analysis was performed to identify and characterize the GRAS gene family of sea buckthorn, especially the comparative genome method was used to demonstrate its different duplication models. We identified 62 sea buckthorn GRAS (HrGRAS) genes and renamed based on their respective chromosome distribution. Fifty-nine HrGRASs were classified into nine subgroups and three HrGRASs did not belong to any of the subfamilies according to their phylogenetic features. HrGRAS genes tend to have a representative GRAS domain, few introns, and unevenly distributed on chromosomes. Seg- mental duplication was the main driver of the GRAS gene family expansion, followed by whole-genome duplication (WGD) and tandem duplication, according to the results of comparative genome analysis. GRAS genes involved in duplication expe- rienced strong purifying selection pressure according to the Ka/Ks ratios. The expression profile derived from transcriptome data shows the expression patterns of HrGRAS gene in different tissues and the characterization of differentially expressed genes. This systematic analysis provided a foundation to understand the expansion and potential functions of GRAS genes with an aim of sea buckthorn crop improvement.

Manganese dioxide-graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Yuxia Liu,Zhenfa Zhang,Cuizong Zhang,Wei Huang,Caiyun Liang,Jinyun Peng 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.8

Excess amount of analgesic and anti-inflammatory drug, such as indomethacin, often leads to serious gastrointestinal complications; therefore, amount of such active compound should be regulated in commercial drugs. This study proposes an efficient analytical technique to detect indomethacin selectively. We prepared and investigated electrochemical properties of a manganese dioxide-graphene nanocomposite film modified glassy carbon electrode (MnO2-Gr/GCE). The behavior of the modified electrode as electrocatalyst towards indomethacin oxidation was also examined. The cyclic voltammetric results reveal that the electrocatalytic activity for the oxidation of indomethacin can significantly be enhanced on the MnO2-Gr/GCE. Indomethacin exhibited a sensitive anodic peak at about 0.90 V at MnO2-Gr/GCE. The data obtained from differential pulse voltammetry showed that the anodic peak currents were linearly dependent on the indomethacin concentrations in the range of 1.0 × 10−7 to 2.5 × 10−5 mol/L with a detection limit of 3.2 × 10−8 mol/L (S/N = 3). Most importantly, the proposed method shows efficient and selective sensing of indomethacin in commercial pharmaceutical formulations. This is the first report of a voltammetric sensor for indomethacin using MnO2-Gr/GCE. We believe that this new method can be commercialized for routine applications in laboratories.

Genome-wide scan for runs of homozygosity identifies candidate genes in Wannan Black pigs

Wu Xudong,Zhou Ren,Zhang Wei,Cao Bangji,Xia Jingjing,Wang Caiyun,Zhang Xiaodong,ChuMing-Xing,Yin Zongjun,Ding Yueyun 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: Runs of homozygosity (ROH) are contiguous lengths of homozygous genotypes that can reveal inbreeding levels, selection pressure, and mating schemes. In this study, ROHs were evaluated in Wannan Black pigs to assess the inbreeding levels and the genome regions with high ROH frequency.Methods: In a previous study, we obtained 501.52 GB of raw data from resequencing (10×) of the genome and identified 21,316,754 single-nucleotide variants in 20 Wannan Black pig samples. We investigated the number, length, and frequency of ROH using resequencing data to characterize the homozygosity in Wannan Black pigs and identified genomic regions with high ROH frequencies.Results: In this work, 1,813 ROHs (837 ROHs in 100 to 500 kb, 449 ROHs in 500 to 1,000 kb, 527 ROHs in >1,000 kb) were identified in all samples, and the average genomic inbreeding coefficient (FROH) in Wannan Black pigs was 0.5234. Sixty-one regions on chromosomes 2, 3, 7, 8, 13, 15, and 16 harbored ROH islands. In total, 105 genes were identified in 42 ROH islands, among which some genes were related to production traits.Conclusion: This is the first study to identify ROH across the genome of Wannan Black pigs, the Chinese native breed of the Anhui province. Overall, Wannan Black pigs have high levels of inbreeding due to the influence of ancient and recent inbreeding due to the genome. These findings are a reliable resource for future studies and contribute to save and use the germplasm resources of Wannan Black pigs. Objective: Runs of homozygosity (ROH) are contiguous lengths of homozygous genotypes that can reveal inbreeding levels, selection pressure, and mating schemes. In this study, ROHs were evaluated in Wannan Black pigs to assess the inbreeding levels and the genome regions with high ROH frequency. Methods: In a previous study, we obtained 501.52 GB of raw data from resequencing (10×) of the genome and identified 21,316,754 single-nucleotide variants in 20 Wannan Black pig samples. We investigated the number, length, and frequency of ROH using resequencing data to characterize the homozygosity in Wannan Black pigs and identified genomic regions with high ROH frequencies. Results: In this work, 1,813 ROHs (837 ROHs in 100 to 500 kb, 449 ROHs in 500 to 1,000 kb, 527 ROHs in >1,000 kb) were identified in all samples, and the average genomic inbreeding coefficient (FROH) in Wannan Black pigs was 0.5234. Sixty-one regions on chromosomes 2, 3, 7, 8, 13, 15, and 16 harbored ROH islands. In total, 105 genes were identified in 42 ROH islands, among which some genes were related to production traits. Conclusion: This is the first study to identify ROH across the genome of Wannan Black pigs, the Chinese native breed of the Anhui province. Overall, Wannan Black pigs have high levels of inbreeding due to the influence of ancient and recent inbreeding due to the genome. These findings are a reliable resource for future studies and contribute to save and use the germplasm resources of Wannan Black pigs.

Effects of Dietary Copper Source and Level on Performance, Carcass Characteristics and Lipid Metabolism in Lambs

Cheng, Jianbo,Fan, Caiyun,Zhang, Wei,Zhu, Xiaoping,Yan, Xiaogang,Wang, Runlian,Jia, Zhihai Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.5

An experiment was conducted to determine the effect of dietary copper (Cu) on performance, carcass characteristics and lipid metabolism in lambs. Fifty DorperMongolia wether lambs (approximately 3 month of age; 23.80.6 kg of body weight) were housed in individual pens and were assigned randomly to one of five treatments. Treatments consisted of 1) control (no supplemental Cu), 2) 10 mg Cu/kg DM from Cu-lysine, 3) 20 mg Cu/kg DM from Cu-lysine, 4) 10 mg Cu/kg DM from tribasic copper chloride (Cu2(OH)3Cl; TBCC), 5) 20 mg Cu/kg DM from tribasic copper chloride. The Cu concentration was 6.74 mg/kg DM in the basal diet. Body weight was measured on two consecutive days at the start and the end of the 60-day experimental period. Blood samples were collected and then the lambs were slaughtered on d 60. Performance was not affected (p>0.05) by dietary Cu treatment. Cu-supplemented and control lambs had similar hot carcass weight, dressing percentage and longissimus muscle area, but Cu supplementation, regardless of source and level, reduced (p<0.01) 12th rib backfat and kidney fat in lambs. Plasma tumor necrosis factor-alpha (TNF-) and serum triglyceride concentrations were increased (p<0.05), total cholesterol concentrations were decreased (p<0.05) and nonesterified fatty acids (NEFA) concentrations tended to be increased (p<0.07) by Cu supplementation. However, Serum concentrations of HDL-cholesterol and LDL-cholesterol were not affected (p>0.05) by dietary treatment. Fatty acid profile of longissimus muscle was similar across treatments. These results indicate that Cu-lysine and TBCC are of similar availability in lambs. Cu supplementation given to DorperMongolia wether lambs altered lipid metabolism. The reduction in backfat depth may be due to copper altering TNF- metabolism in lambs. Supplementation of 10 or 20 mg Cu/kg DM showed similar effects on lipid metabolism in lambs.

Inactivation of Vibrio parahaemolyticus by Aqueous Ozone

( Lifang Feng ),( Kuo Zhang ),( Mengsha Gao ),( Chunwei Shi ),( Caiyun Ge ),( Daofeng Qu ),( Junli Zhu ),( Yugang Shi ),( Jianzhong Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.8

Vibrio parahaemolyticus contamination causes serious foodborne illness and has become a global health problem. As a disinfectant, aqueous ozone can effectively kill a number of bacteria, viruses, parasites, and other microorganisms. In this study, three factors, namely, the aqueous ozone concentration, the exposure time, and the bacterial density, were analyzed by response surface methodology, and the aqueous ozone concentration was the most influential factor in the sterilization ratio. Under low aqueous ozone concentrations (less than 0.125 mg/l), the bacterial cell membranes remained intact, and the ozone was detoxified by intracellular antioxidant enzymes (e.g., superoxide dismutase and catalase). Under high aqueous ozone concentrations (more than 1 mg/l), cell membranes were damaged by the degree of peripheral electronegativity at the cell surface and the concentration of lactate dehydrogenase released into the extracellular space, and the ultrastructures of the cells were confirmed by transmission electron microscopy. Aqueous ozone penetrated the cells through leaking membranes, inactivated the enzymes, inhibited almost all the genes, and degraded the genetic materials of gDNA and total RNA, which eventually led to cell death.

Benzidine Induces Epithelial–Mesenchymal Transition of Human Bladder Cancer Cells through Activation of ERK5 Pathway

Xin Sun,Tao Zhang,Qifei Deng,Qirui Zhou,Xianchao Sun,Enlai Li,Dexin Yu,Caiyun Zhong 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.3

Benzidine, a known carcinogen, is closely associated with the development of bladder cancer (BC). Epithelial–mesenchymal transition (EMT) is a critical pathophysiological process in BC progression. The underlying molecular mechanisms of mitogen-activated protein kinase (MAPK) pathway, especially extracellular regulated protein kinases 5 (ERK5), in regulating benzidine-induced EMT remains unclarified. Hence, two human bladder cell lines, T24 and EJ, were utilized in our study. Briefly, cell migration was assessed by wound healing assay, and cell invasion was determined by Transwell assay. Quantitative PCR and western blot were utilized to determine both gene expressions as well as protein levels of EMT and MAPK, respectively. Small interfering RNA (siRNA) was transfected to further determine ERK5 function. As a result, the migration and invasion abilities were enhanced, epithelial marker ex-pression was decreased while mesenchymal marker expression was increased in human BC cell lines. Meanwhile, benzidine administration led to activation of ERK5 and activator protein 1 (AP-1) proteins, without effective stimulation of the Jun N-terminal kinase (JNK) or p38 pathways. Moreover, Benzidine-induced EMT and ERK5 activation were completely suppressed by XMD8-92 and siRNAs specific to ERK5. Of note, ERK1/2 was acti-vated in benzidine-treated T24 cells, while benzidine-induced EMT could not be reversed by U0126, an ERK1/2 inhibitor, as indicated by further study. Collectively, our findings revealed that ERK5-mediated EMT was critically involved in benzidine-correlated BC progression, indicating the therapeutic significance of ERK5 in benzidine-related BC.

Insights into the evolutionary origin and expansion of the BBX gene family

Yu Liyang,Lyu Zhongrui,Liu Hong,Zhang Guoyun,He Caiyun,Zhang Jianguo 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.2

The B-box proteins are a class of zinc finger transcription factors and play important roles in regulating plant growth, devel- opment, and stress response. However, their origin and expansion model in plants have been very vague. In this study, 208 B-box genes were identified in 18 plant species, and phylogenetic analyses divided them into five structure groups. Subse- quently, the sequence analysis including theoretical isoelectric point, instability index, and aliphatic index showed the wide variation of B-box gene in different species. Our multispecies genome-wide analysis reveals that the structure groups III and IV in the plant BBX gene family have the earliest origin (Rhodophyta) and are extensively expanded in land plants, while the other three structure groups (I, II, and V) seem to originate at least in the last common ancestor of land plants. Further- more, whole genome duplication (WGD) was the main driver (28 gene pairs, 65.12%) of the B-box gene family expansion, followed by segmental duplication, which tend to have more introns and are subject to more intense purification selections. We also analyzed the sequence differences between B-box domains to propose a new evolutionary model of B-box domain. These analyses provide new insights for understanding the origin and evolution of the B-box gene family.