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Cha, Byeongjin,Han, Sangsub The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.2
Using phytoplasma universal primer pair Pl and P7, a fragment of about 1.8 kb nucleotide sequences of 16S rRNA gene and 16S-23S rRNA intergenic spacer region, and a portion of 23S rRNA gene of jujube witches'broom (JWB) and mulberry dwarf(MD) phytoplasmas were determined. The nucleotide sequences of JWB and MD were 1,850 bp and 1,831 bp long, respectively. The JWB phytoplasma sequence was aligned with the homologous sequence of MD phytoplasma. Twenty-eight base insertions and nine base deletions were found in the JWB phytoplasma sequence compared with that of MD phytoplasma. The similarity of the aligned sequences of JWB and MD was 84.8%. The near-complete 16S rRNA gene DNA sequences of JWB and MD were 1,529 bp and 1,530 bp in length, respectively, and revealed 89.0% homology. The 16S-23S rRNA intergenic spacer region DNA sequences were 263 bp and 243 bp in lengths respectively, while homology was only 70% and the conserved tRNA-lle gene of JWB and MD was located into the intergenic space region between 16S-23S rRNA gene. The nucleotide sequences were 77 bp long in both JWB and MD, and showed 97.4% sequence homology. Based on the phylogenetic analysis of the two phytoplasmas, the JWB phytoplasma belongs to the Elm yellow phytoplasma group (16S rV), whereas, the MD phytoplasma belongs to the Aster yellow group (16S rI).
Cha, Byeongjin,Han, Sangsub The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.6
Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf(MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100% homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.
Host Range of Cryphonectria parasitica in Korea
Yu-Liang Gao,Byungkwan Kim,Byeongjin Cha 忠北大學校 農業科學硏究所 2016 農業科學硏究 Vol.32 No.2
Cryphonectria parasitica, the pathogen of chestnut blight, was isolated from stem cankers on chestnut trees and it’s host range was investigated on 25 species of various woody plants. Tested trees were inoculated with mycelial plug of C. parasitica through wound on the bark. Among the trees, Quercus aliena, Q. dentata, Q. mongolica, and Q. serrata developed brown discoloration on inner surface of the bark and were determined as new host trees for C. parasitica. Besides the Quercus species, linden tree and white mulberry were infected with the pathogen at less severity and regarded as the reservoir hosts of C. parasitica. When linden tree and white mulberry was inoculated with the isolate bW111, the pathogen could be isolated again, but showed a little difference in colony morphology, vegetative compatibility, and dsRNA size. Re-isolated C. parasitica also showed difference in pathogenicity in the inoculation test and caused the lesions larger than that of bW111 on the stem of chestnut trees. Several new host trees and pathogenicity variation of C. parasitica were reported. It should be taken into account in the analyses of chestnut blight epidemiology and/or developing biological control strategy
차병진,조일찬 충북대학교 한국과학재단 지정 첨단원예기술개발연구센터 1996 연구보고서 Vol.1 No.-
오이의 잎으로부터 미생물들을 분리한 결과, Aspergillus sp., Pennicillium sp., Rhizopus sp.등 14종의 곰팡이와 10종의 세균들을 분리하였으며, 현재 동정실험을 하고 있는 중이다. 동시에 이들을 오이 잎에 접종한 다음 흰가루병균을 접종하여 흰가루병균에 대한 길항력을 알아보고 있는 중이다. 또한, 길항균은 오이 뿐 아니라 흰가루병균의 여러 가지 기주식물들로부터 계속하여 분리 중이다. 흰가루병균의 분생포자는 물한천배지 상에서 수용성 규소의 농도에 관계없이 모두 비슷한 정도로 발아하였으며, 파괴되는 포자의 비율도 매우 낮았다. 오이의 잎에 0, 2, 4, 8, 16mM이 수용성 규소를 충분히 분무한 다음 흰가루병균의 분생포자들을 접종한 결과, 잎에 생기는 균총의 수에 있어 큰 차이를 보였는데, 4mM 처리에서 균총의 수가 가장 적었고, 그 다음이 2, 8, 16mM의 순이었으며, 규소 무처리 대조구에서는 처리구에서보다 더 많았다.
차병진,Cha, Byeongjin 한국식물병리학회 2017 식물병연구 Vol.23 No.3
In December of 2016, 'The Forest Protection Act' was amended partly in The National Assembly and the socalled 'Tree Doctor Act' was promulgated. Tree Doctor Act will be enforced from June 28, 2018. Under the new Act, none other than 'Tree Hospital' can do disease and pest management work for trees in public living space. The only exclusive qualification for tree hospital is a 'Tree Doctor', the government registered license which is newly established by the Act. To become a tree doctor, he/she must complete the tree doctor training courses in the designated 'Tree Doctor Academy' and pass the qualification test. Currently, Korea Forest Service is drafting the enforcement ordinances and regulations for the implement of Tree Doctor Act. When taking into consideration that the most fundamental and important discipline of the plant and tree health care is the plant pathology, and that the tree health care is a promising business for young plant pathology people, Korean Society of Plant Pathology is ought to be actively involved in the preparation of the enforcement ordinances and regulations, and help the early establishment of the new tree health care system in living spaces of Korea. 2016년 12월에 산림보호법 중 일부가 개정되어 이른바 '나무의사법'이 공포되었으며, 이 법은 2018년 6월 28일부터 시행될 예정이다. 새로운 법안에서는 나무의사 자격 소지자에 한하여 나무병원을 개업할 수 있고, 나무병원만이 생활권 공공분야의 수목병해충 관리를 할 수 있도록 규정하고 있으며, 그에 따라 '나무의사'라는 국가공인자격이 신설되었다. 나무의사가 되기 위해서는 지정된 양성과정을 이수하고 시험을 통과하여야 한다. 현재 산림청에서는 이 법의 시행을 위하여 구체적 시행방안을 포함하는 시행령과 시행규칙 등을 만들고 있다. 수목진료 및 건강관리에서 가장 핵심적인 부분이 식물병리학이라는 사실을 감안할 때, 그리고 식물병리학을 공부하는 학생들의 사회진출을 위하여 식물병리학회는 새로운 수목진료체계가 정착될 수 있도록 나무의사 양성기관의 교육과정 개발 및 나무의사 선발시험계획 수립 등에 적극적으로 관여하여야 할 것으로 생각한다.
차병진,서상태,한상섭,Byeongjin Cha,Sang-Tae Seo,Sang-sup Han 한국식물병리학회 2024 식물병연구 Vol.30 No.1
Since the List of Plant Diseases in Korea (DisList) was first published in 1986, the 6th edition appeared 36 years later. In 2023, the 6.1 edition, a revised and improved version of the 6th edition, was released to the public on the web free of charge. The contents of DisList increased, with the number of hosts increasing from 437 taxa to 1,420 taxa and the number of disease species increasing from 1,539 to 6,680. Among these, tree diseases are 3,586 species and their hosts include 504 taxa, providing much help to experts who need them. Meanwhile, the importance of accurate disease names continues to grow with the legalization of tree care, but many disease names are still inappropriate or misused, causing confusion. Disease names that do not follow the naming regulations are still registered, and even if the same pathogen infects hosts of the same taxa, the disease names are given differently, and there are many disease names that do not indicate the characteristics of the disease. Also, there are diseases reported without Korean names. In order to make DisList better, the review committee for disease names should establish the regulations to review and register disease names, and establish a system to review new disease names before publishing papers.