Pgis Tool for Erosion Susceptibility and Soil Conservation Planning in a Watershed of Nepal

Krishna Prasad Bhandari,Prem Sagar Bhandari 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Analysis and characterization of iron pyrite nanocrystals and nanocrystalline thin films derived from bromide anion synthesis

Bhandari, Khagendra P.,Roland, Paul J.,Kinner, Tyler,Cao, Yifang,Choi, Hyekyoung,Jeong, Sohee,Ellingson, Randy J. The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.13

<▼1><P>We use a solution-based bromide anion hot injection method to synthesize stable, phase pure and highly crystalline cubic iron pyrite (FeS2) nanocrystals, with size varying from ∼70 to ∼150 nm.</P></▼1><▼2><P>We use a solution-based hot injection method to synthesize stable, phase pure and highly crystalline cubic iron pyrite (FeS2) nanocrystals, with size varying from ∼70 to 150 nm. We use iron(ii) bromide as an iron precursor, elemental sulfur as the sulfur source, trioctylphosphine oxide (TOPO) and 1,2-hexanediol as capping ligands, and oleylamine (OLA) as a non-coordinating solvent during the synthesis. We report on the influence of hydrazine treatment, and of thermal sintering, on the morphological, electronic, optical, and surface chemical properties of FeS2 films. Four point probe and Hall measurements indicate that these iron pyrite films are highly conductive. Although they are unsuitable as an effective photovoltaic light-absorbing layer, they offer clear potential as a conducting contact layer in photovoltaic and other optoelectronic devices.</P></▼2>

Ripening-Dependent Changes in Antioxidants, Color Attributes, and Antioxidant Activity of Seven Tomato ( <i>Solanum lycopersicum</i> L.) Cultivars

Bhandari, Shiva Ram,Lee, Jun Gu Hindawi Publishing Corporation 2016 Journal of analytical methods in chemistry Vol.2016 No.-

<P>To evaluate the ripening-dependent changes in phytonutrients, seven commercial cultivars (two general and five cherry) of tomatoes were cultivated under greenhouse conditions. Fruits were harvested at breaker, turning, pink, light red, and red stages of each cultivar, and antioxidant contents, color attributes, and antioxidant activities were measured. During ripening process, lycopene content increased from the breaker to red stage, while lutein displayed the reverse accumulation pattern, with higher values during the breaker stage. In contrast, <I>β</I>-carotene showed the highest levels of synthesis in pink and light red stages. Furthermore, flavonoids (quercetin, rutin, naringenin, and luteolin) also showed similar ripening-dependent changes, with higher quantities in pink and light red stages. Ascorbic acid showed continuously increasing patterns throughout ripening until the red stage, while the accumulation of total phenolics was cultivar-dependent. These results indicate that each antioxidant compound has a unique pattern of accumulation and degradation during the ripening process. “Unicon” exhibited highest total carotenoid (110.27 mg/100 g), total phenol (297.88 mg GAE/100 g) and total flavonoid content (273.33 mg/100 g), and consequently highest antioxidant activity (2552.4 <I>μ</I>mol TE/100 g) compared to other cultivars. Throughout the ripening processes, total phenolics showed the highest correlation with antioxidant activity, followed by <I>β</I>-carotene and total flavonoids. In conclusion, ripening in tomatoes is accompanied by incremental increases in various antioxidant compounds to some extent, as well as by concomitant increases in antioxidant activity.</P>

Evaluation of Skin Permeation and Accumulation Profiles of a Highly Lipophilic Fatty Ester

Bhandari, Krishna Hari,Lee, Dong-Xun,Newa, Madhuri,Yoon, Sung-Il,Kim, Jung-Sun,Kim, Dae-Duk,Kim, Jung-Ae,Yoo, Bong-Kyo,Woo, Jong-Soo,Lyoo, Won-Seok,Lee, Jae-Hwi,Choi, Han-Gon,Yong, Chul-Soon 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2

The aim was to evaluate the skin permeation and accumulation profiles of a highly lipophilic fatty ester using the combination of various permeation enhancing techniques to study the potential of highly lipophilic fatty esters as local topical agents. Permeation and accumulation profiles of ketorolac stearate (C18:0) were studied using solubility improved formulation, supersaturated solution of permeant in enhancer vehicle, lipophilic receptor solution, enhancer pretreatment, and the removal of stratum corneum and delipidization of skins. Impermeability and minimal skin accumulation of ketorolac stearate could delineate a preliminary possibility for designing safer topical agents without systemic absorption.

The regulatory role of c-MYC on HDAC2 and PcG expression in human multipotent stem cells

Bhandari, Dilli Ram,Seo, Kwang-Won,Jung, Ji-Won,Kim, Hyung-Sik,Yang, Se-Ran,Kang, Kyung-Sun Blackwell Publishing Ltd 2011 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.15 No.7

<P><B>Abstract</B></P><P>Myelocytomatosis oncogene (c-MYC) is a well-known nuclear oncoprotein having multiple functions in cell proliferation, apoptosis and cellular transformation. Chromosomal modification is also important to the differentiation and growth of stem cells. Histone deacethylase (HDAC) and polycomb group (PcG) family genes are well-known chromosomal modification genes. The aim of this study was to elucidate the role of c-MYC in the expression of chromosomal modification <I>via</I> the HDAC family genes in human mesenchymal stem cells (hMSCs). To achieve this goal, c-MYC expression was modified by gene knockdown and overexpression <I>via</I> lentivirus vector. Using the modified c-MYC expression, our study was focused on cell proliferation, differentiation and cell cycle. Furthermore, the relationship of c-MYC with HDAC2 and PcG genes was also examined. The cell proliferation and differentiation were checked and shown to be dramatically decreased in c-MYC knocked-down human umbilical cord blood-derived MSCs, whereas they were increased in c-MYC overexpressing cells. Similarly, RT-PCR and Western blotting results revealed that HDAC2 expression was decreased in c-MYC knocked-down and increased in c-MYC overexpressing hMSCs. Database indicates presence of c-MYC binding motif in HDAC2 promoter region, which was confirmed by chromatin immunoprecipitation assay. The influence of c-MYC and HDAC2 on PcG expression was confirmed. This might indicate the regulatory role of c-MYC over HDAC2 and PcG genes. c-MYCs’ regulatory role over HDAC2 was also confirmed in human adipose tissue-derived MSCs and bone-marrow derived MSCs. From this finding, it can be concluded that c-MYC plays a vital role in cell proliferation and differentiation <I>via</I> chromosomal modification.</P>

Cytogenetic Profile of De Novo B lineage Acute Lymphoblastic Leukemia: Determination of Frequency, Distribution Pattern and Identification of Rare and Novel Chromosomal Aberrations in Indian Patients

Bhandari, Prerana,Ahmad, Firoz,Dalvi, Rupa,Koppaka, Neeraja,Kokate, Prajakta,Das, Bibhu Ranjan,Mandava, Swarna Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Chromosomal aberrations identified in acute lymphoblastic leukemia (ALL) have an important role in disease diagnosis, prognosis and management. Information on karyotype and associated clinical parameters are essential to physicians for planning cancer control interventions in different geographical regions. Materials and Methods: In this study, we present the overall frequency and distribution patterns of chromosomal aberrations in both children and adult de novo B lineage ALL Indian patients using conventional cytogenetics, interphase FISH and multiplex RT-PCR. Results: Among the 215 subjects, cytogenetic results were achieved in 172 (80%) patients; normal karyotype represented 37.2% and abnormal 62.8% with a distribution as follows: 15.3% hypodiploidy; 10.3% hyperdiploidy; 15.8% t(9;22); 9.8% t(1;19); 3.7% t(12;21); 2.8% t(4;11); 2.8% complex karyotypes. Apart from these, we observed several novel, rare and common chromosomal rearrangements. Also, FISH studies using LSI extra-signal dual-color probes revealed additional structural or numerical changes. Conclusions: These results demonstrate cytogenetic heterogeneity of ALL and confirm that the incidence of chromosomal abnormalities varies considerably. To the best of our knowledge, this is one of the largest reported series of cytogenetic investigations in Indian B-lineage ALL cases. In addition, ongoing cytogenetic studies are warranted in larger groups of B-lineage ALL cases to identify newly acquired chromosomal abnormalities that may contribute to disease diagnosis and management.

A novel off-grid hybrid power system comprised of solar photovoltaic, wind, and hydro energy sources

Bhandari, B.,Lee, K.T.,Lee, C.S.,Song, C.K.,Maskey, R.K.,Ahn, S.H. Applied Science Publishers 2014 APPLIED ENERGY Vol.133 No.-

Several factors must be considered before adopting a full-phase power generation system based on renewable energy sources. Long-term necessary data (for one year if possible) should be collected before making any decisions concerning implementation of such a systems. To accurately assess the potential of available resources, we measured solar irradiation, wind speed, and ambient temperature at two high-altitude locations in Nepal: the Lama Hotel in Rasuwa District and Thingan in Makawanpur District. Here, we propose two practical, economical hybridization methods for small off-grid systems consisting entirely of renewable energy sources-specifically solar photovoltaic (PV), wind, and micro-hydro sources. One of the methods was tested experimentally, and the results can be applied to help achieve Millennium Development Goal 7: Ensuring environmental sustainability. Hydro, wind, and solar photovoltaic energy are the top renewable energy sources in terms of globally installed capacity. However, no reports have been published about off-grid hybrid systems comprised of all three sources, making this implementation the first of its kind anywhere. This research may be applied as a practical guide for implementing similar systems in various locations. Of the four off-grid PV systems installed by the authors for village electrification in Nepal, one was further hybridized with wind and hydro power sources. This paper presents a novel approach for connecting renewable energy sources to a utility mini-grid.

Comparisons of Nutritional and Phytochemical Property of Genetically Modified CMV-resistant Red Pepper and Its Parental Cultivar

Bhandari, Shiva Ram,Basnet, Sunita,Chung, Kyu-Hwan,Ryu, Ki-Hyun,Lee, Young-Sang 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2

The aim of this study was to compare the nutritional composition of a genetically modified (GM) CMV-resistant red pepper strain with its parental line. Specifically, the nutrient content (moisture, protein, lipid, ash, carbohydrate, and energy), minerals, fatty acid composition, capsaicinoids (capsaicin and dihydrocapsaicin), free sugars (glucose, sucrose, and fructose), vitamin E isomers (${\alpha}$-, ${\beta}$-, ${\gamma}$-, and ${\delta}$-tocopherols), vitamin C, phytosterols (campesterol, stigmasterol, and ${\beta}$-sitosterol), squalene contents, and ASTA values were analyzed and compared. Most of the analyzed compounds showed no significant differences between the GM red peppers and the parental line. The only significant difference was observed in stigmasterol content, but the difference was below the 15% natural-fluctuation threshold. These results suggest that the CMV-GM pepper is equivalent to its parental line in terms of nutritional and phytochemical composition.

A NOTE ON LEVITZKI RADICAL OF NEAR-RING

Bhandari, M.C.,Sexana, P.K. Department of Mathematics 1980 Kyungpook mathematical journal Vol.20 No.2

Phytonutrient Profile of Purple Perilla (Perilla frutescens var. crispa) Seeds

Bhandari, Shiva Ram,Lee, Ju-Kyong,Lee, Young-Sang The Korean Society of Crop Science 2011 한국작물학회지 Vol.56 No.3

To characterize phytonutrients, the seeds of 12 purple Perilla (Perilla frutescens var. crispa) accessions collected from Korea and Japan were used for quantitative analysis of tocopherols, phytosterols, squalene and fatty acids. The average tocopherol, squalene and phytosterols contents were 12.2 mg $100g^{-1}$, 3.99 mg $100g^{-1}$ and 77.20 mg $100g^{-1}$, respectively. Among 4 tocopherol (T) isomers (${\alpha}$-T, ${\beta}$-T, ${\gamma}$-T, and ${\delta}$-T), ${\gamma}$-T was present in the highest quantity (11.03 mg $100g^{-1}$) with the least variation (CV = 13.7%), while ${\beta}$-T was present in lowest quantity (0.25 mg 1$100g^{-1}$). Compared to campesterol (4.36 mg $100g^{-1}$) and stigmasterol (13.32 mg $100g^{-1}$), ${\beta}$-sitosterol exhibited higher quantity (59.51 mg $100g^{-1}$) with 9.5% of variation. The major fatty acids were unsaturated fatty acids such as linolenic (61.5%), linoleic (17.3%), and oleic (9.9%) acids compared to saturated ones: palmitic (7.6%) and stearic (3.7%) acids. When Korean and Japanese accession were compared, almost no difference in content could be observed, while more variation as evaluated by CV (%) could be observed in Japanese accession in most phytonutrients suggesting wider genetic variation of purple Perilla in Japan. Presence of all above-mentioned phytonutrient compounds strongly suggested health beneficial value of purple Perilla seeds.