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        Mitochondrial DNA-based investigation of dead rorqual (Cetacea: Balaenopteridae) from the west coast of India

        Shantanu Kundu,Manokaran Kamalakannan,Dhriti Banerjee,Flandrianto Sih Palimirmo,Arif Wibowo,Hyun-Woo Kim The Korean Society of Fisheries and Aquatic Scienc 2024 Fisheries and Aquatic Sciences Vol.27 No.1

        The study assessed the utility of mitochondrial DNA for identifying a deceased rorqual discovered off the western coast of India. Both the COI and Cytb genes exhibited remarkable 99-100% similarity with the GenBank sequence of Balaenoptera musculus through a global BLAST search, confirming their affiliation with this species. Inter-species genetic distances for COI and Cytb genes ranged from 6.75% to 9.80% and 7.37% to 10.96% respectively, compared with other Balaenopteridae species. The Bayesian phylogenies constructed based on both COI and Cytb genes demonstrated clear and separate clustering for all Balaenopteridae species, further reaffirming their distinctiveness, while concurrently revealing a cohesive clustering pattern of the generated sequences within the B. musculus clade. Beyond species confirmation, this study provides valuable insights into the presence of live and deceased B. musculus individuals within Indian marine ecosystems. This information holds significant potential for guiding conservation efforts aimed at safeguarding Important Marine Mammal Areas (IMMAs) in India over the long term.

      • KCI등재

        DNA barcoding reveals host associated genetic diversity of ‘tea mosquito bug’ Helopeltis theivora (Miridae: Heteroptera) from India

        K.N. Chandrashekara,R. Raj Kumar,Vikas Kumar,Dhriti Banerjee,Shantanu Kundu,Biswatosh Ghosh,Kaomud Tyagi 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

        The tea mosquito bug, Helopeltis theivora Waterhouse (Miridae: Heteroptera) is a polyphagous pest that affects a number of economically important crops in India. Partial sequences of mitochondrial cytochrome c oxidase 1 (mtCOI) were used to study host associated genetic variation on H. theivora collected from tea (Camellia sinensis L.), cashew (Anacardiumoccidentale L.), acalypha (Acalypha indica L.) and cocoa (Theobroma cacao L.). The results suggest that host plants have a significant effect on the genetic variation of bugs collected from two wide geographical locations (South India and North-East India). Haplotyping data analysis of mtCOI reveals six host plant specific haplotypes of H. theivorawith minimumgenetic divergence (0.8%) between two haplotypeswithin tea as compared to other plants (2.6–11.1%). Further phylogenetic (NJ, ML and BA) analysis evidence three host specific distinct clades. The most surprising result that emerged from our studies is the harmony between clustering of H. theivora and host plant phylogeny. The results indicate that host plants do have selection pressure and substantial effect on genetic variation in H. theivora and such adaptive evolution may have repercussions for IPM (Integrated Pest Management) strategies of H. theivora in India.

      • KCI등재

        Field larvicidal and oviposition repellent efficacy of three essential oil formulations for the control of filaria vector mosquito Culex quinquefasciatus Say (Diptera: Culicidae): A kill and push concept

        Suman Laxmi Narayan,De Souvik,Sharma Gaurav,Mandal Udita,Bhattacherjee Rashmi,Banerjee Dhriti,Suman Devi Shankar 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

        Biological control of larval mosquitoes is in great demand due to the development of resistance against synthetic insecticides, environmental toxicity and the inability to protect habitats from further oviposition. In the present study, three botanical essential oils (BEOs) – citronella, eucalyptus, and pine oils – were formulated for the assessment of larvicidal and oviposition repellent efficacies against Culex quinquefasciatus Say, the filaria vector. The GC–MS profiling of BEOs showed the presence of 16 – 19 compounds covering 87.7–93% of oil composition. The resistance status of Culex quinquefasciatus population was evaluated with temephos (LC 50 = 0.001 ppm, LC 90 = 0.01 ppm). Larval bioassay of emulsifiable concentrate (EC) formulations prepared from eucalyptus and pine oils showed promising efficacy (LC 50 = 22.7 and 23.2 ppm) and LC 90 (63.8 and 62.4 ppm) compared to citronella oil EC (LC 50 = 43.4 ppm and LC 90 = 199.0 ppm). The field trials of eucalyptus + pine (1:1 ratio) EC showed 100% larval mortality for 3 weeks at 300 ppm compared to 2 weeks of individual oils. Further, the oviposition attraction index (OAI) for ECs of eucalyptus, pine, and their combination showed complete protection of breeding habitats from oviposition at 1st week and − 0.9 to − 1.0 OAI at 2nd week with slight reduction to − 0.5 at 3rd week. Citronella EC provided shortest larvicidal and oviposition repellent efficacy under the field con ditions. The promising mosquitocidal activities of EC formulations of eucalyptus and pine or their combination suggest them as potential biocontrol vector control candidates over citronella oil.

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