Cryoprotectant Effect of Trehalose on Frozen-Thawed Boar Sperm

Baek SY,Choi TJ,Kim YM,Cho ES,Soh HC,Cho KH,Chung HJ 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

The objective of this study was to investigate the influence of the addition of cryoprotectants to freezing extenders on post thawing boar sperm quality as well as investigate their optimum concentrations. To evaluate the effect of trehalose (0, 80, 100, 120 mM) added in egg yolk extender freezing medium on motion characteristics, motility, viability and DNA integrity of spermatozoa at post thawing of cryopreservation. Semen were collected from 10 boars and frozen on the same day. Qualified semen samples (motility >80%) from each boar were subdivided four groups, 0 (control), 80 mM (T1), 100 mM (T2) and 12 0 mM (T3). Motility was assessed for % motile cell characteristics using computer-assisted semen analysis (CASA; SAIS SI-100, Medical supply, Korea). Frozen boar sperms were thawed in Beltsville Thawing Solution (BTS) then incubated at 38 celsius degree for 20 minutes. Computer progressive motility, average path velocity, straight line velocity, curvilinear velocity and total motility were higher (p<0.05) in adding 120 mM treatment group (T3) as compared with control. Moreover, trehalose addition did not damage DNA integrity of spermatozoa. Nevertheless, trehalose concentration treatment did not affect the viability of the spermatozoa as the concentration increased. The data showed that trehalose as an additive cryoprotectant was able to improve sperm quality without on sperm DNA integrity in cryopreservation.

Cryoprotectant Effect of Pentoxifylline on Boar Spermatozoa Cryopreserved by the Liquid Nitrogen Vapor Method

Baek SY,Chung HJ,Kim YG,Park CY,Ahn HW,Kim HS,Choi TJ,Kim YM,Cho ES,Kim YS,Cho KH 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

The objective of this study was to investigate the influence of the addition of cryoprotectants to freezing extenders before semen cryopreservation on boar sperm quality as well as investigate their optimum concentrations. To evaluate the effect of pentoxifylline (0, 5, 10, 20 mM) added in egg yolk extender freezing medium on motion characteristics, motility, viability and acrosomal morphology of spermatozoa at post thawing of cryopreservation. Semen were collected from 12 boars and frozen on the same day. Qualified semen samples (motility>80%) from each boar were subdivided four groups, 0 (control), 5 mM (T1), 10 mM (T2) and 20 mM (T3). Motility was assessed for % motile cell characteristics using computer-assisted semen analysis (CASA; SAIS SI-100, Medical supply, Korea). Frozen sperms were thawed in Beltsville Thawing Solution (BTS) then incubated at 38℃ for 20 minutes. Progressive motility, average path velocity, straight line velocity, curvilinear velocity and total motility were higher (p<0.05) in 0 mM (control group) as compared with pentoxifylline treated group. Moreover, pentoxifylline addition did not damage acrosomal morphogenic effect of spermatozoa. Nevertheless, pentoxifylline concentration treatment did not affect the viability of the spermatozoa as the concentration increased. The data showed that pentoxifylline as an additive cryoprotectant was not able to improve sperm motility but also quality in cryopreservation.

Effect of alkaloid of caffeine on frozen-thawed boar sperm quality

Baek SY,Kim YG,Bang DS,Kim SU,Oum JC,Soh HC,Choi TJ,Kim YM,Cho ES,Jung YD,Cho KH,Chung HJ 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

The objective of this study was to investigate the influence of the addition of caffeine (alkaloid family) to the ejaculates of boar sperm quality as well as investigate their optimum concentrations for increasing the movement of sperms. Semen was collected from 9 boars by the gloved-hand technique one week interval. Semen followed by cryopreservation with egg yolk extender freezing medium using freezing protocol. The collected semen were frozen on the same day. Motility was assessed for % motile cell characteristics using computer-assisted semen analysis (CASA; SAIS SI-100, Medical supply, Korea). Frozen boar sperms were thawed in Beltsville Thawing Solution (BTS) with 5, 10, and 15mM caffeine were then incubated at 38 celsius degree for 20 minutes. In experiment 1, semen were diluted BTS and addition of different concentration of caffeine to the pre-freeze semen cryopreservation. In experiment 2, incubation of frozen-thawed sperm in BTS supplemented with different concentration of caffeine for 20 minutes improved (P<0.05) after semen cryopreservation-thawing on sperm quality. After thawing significantly increased progressive and total motility. The addition of 10 mM caffeine to cryopreserved semen after thawing significantly increased progressive and total motility compared with other treatment. These result suggest that caffeine enhanced post-thaw motility of cryopreserved boar sperm when added after thawing.

Solidification/stabilization of ASR fly ash using Thiomer material: Optimization of compressive strength and heavy metals leaching

Baek, Jin Woong,Choi, Angelo Earvin Sy,Park, Hung Suck Elsevier 2017 Waste management Vol.70 No.-

<P><B>Abstract</B></P> <P>Optimization studies of a novel and eco-friendly construction material, Thiomer, was investigated in the solidification/stabilization of automobile shredded residue (ASR) fly ash. A D-optimal mixture design was used to evaluate and optimize maximum compressive strength and heavy metals leaching by varying Thiomer (20–40wt%), ASR fly ash (30–50wt%) and sand (20–40wt%). The analysis of variance was utilized to determine the level of significance of each process parameters and interactions. The microstructure of the solidified materials was taken from a field emission-scanning electron microscopy and energy dispersive X-ray spectroscopy that confirmed successful Thiomer solidified ASR fly ash due to reduced pores and gaps in comparison with an untreated ASR fly ash. The X-ray diffraction detected the enclosed materials on the ASR fly ash primarily contained sulfur associated crystalline complexes. Results indicated the optimal conditions of 30wt% Thiomer, 30wt% ASR fly ash and 40wt% sand reached a compressive strength of 54.9MPa. For the optimum results in heavy metals leaching, 0.0078mg/LPb, 0.0260mg/L Cr, 0.0007mg/LCd, 0.0020mg/L Cu, 0.1027mg/L Fe, 0.0046mg/L Ni and 0.0920mg/L Zn were leached out, being environmentally safe due to being substantially lower than the Korean standard leaching requirements. The results also showed that Thiomer has superiority over the commonly used Portland cement asa binding material which confirmed its potential usage as an innovative approach to simultaneously synthesize durable concrete and satisfactorily pass strict environmental regulations by heavy metals leaching.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Innovative synthesis of Thiomer solidified ASR fly ash. </LI> <LI> D-optimal mixture design of compressive strength and heavy metals leaching. </LI> <LI> Thiomer as a binding material exhibited superiority over ordinary Portland cement. </LI> <LI> Heavy metals were converted to metal sulfides/sulfates upon utilization of Thiomer. </LI> <LI> Thiomer proves to be a novel green construction material. </LI> </UL> </P>

Suppression of PU.1-linked TLR4 expression by cilostazol with decrease of cytokine production in macrophages from patients with rheumatoid arthritis : Inhibition of TLR4 by cilostazol in RA macrophages

Park, SY,Lee, SW,Baek, SH,Lee, CW,Lee, WS,Rhim, BY,Hong, KW,Kim, CD Wiley (Blackwell Publishing) 2013 British journal of pharmacology Vol.168 No.6

Application of the ML-EM algorithm in the suppression of Compton background of gamma-ray spectroscopy system

Hoang, Sy Minh Tuan,Sun, Gwang Min,Baek, Hani,Kim, Jiseok Springer-Verlag 2016 JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY Vol.307 No.3

Seasonal Cycle of Phytoplankton in Aquaculture Ponds in Bangladesh

Affan, Abu,Jewel, Abu Syed,Haque, Mahfuzul,Khan, Saleha,Lee, Joon-Baek The Korean Society of Phycology 2005 ALGAE Vol.20 No.1

A study on the seasonal changes in the phytoplankton community was carried out in four aquaculture ponds of Bangladesh over a period of 16 months from August 2000 to November 2001. Out of 45 phytoplankton species identified, 30 belong to Cyanophyceae, 7 to Chlorophyceae, 5 to Bacillariophyceae and 3 to Euglenophyceae. The highest phytoplankton abundance was observed in spring followed by early autumn, summer, and the lowest was in winter. The annual succession of Cyanophyceae was characterized by spring and early autumn period dominated by Microcystis sp. Anabaena sp. and Planktolymbya sp. with Microcystis sp. as the main blue-green algae represented. Chlorophyceae was characterized by rainy season domination of Chlorella vulgaris, Pediastrum sp. and Scenedesmus denticulatus with maximum abundance of Chlorella vulgaris. Whereas Bacillariophyceae was dominant during the winter period. Navicula angusta and Cyclotella meneghiniana were the most frequently occurring species of Bacillariophyceae throughout the study period. Euglenophyceae was dominant in late autumn and Euglena sp. was the dominant species. The effect of various physicochemical water quality parameters on the seasonal distribution and succession of the above mentioned phytoplankton population as well as the interaction and eutrophication are discussed.

Seasonal cycle of phytoplankton in aquaculture ponds in Bangladesh

Abu Affan,Joon-Baek Lee,Saleha Khan,Mahfuzul Haque,Abu Syed Jewel 한국조류학회I 2005 ALGAE Vol.20 No.1

A study on the seasonal changes in the phytoplankton community was carried out in four aquaculture ponds of Bangladesh over a period of 16 months from August 2000 to November 2001. Out of 45 phytoplankton species identified, 30 belong to Cyanophyceae, 7 to Chlorophyceae, 5 to Bacillariophyceae and 3 to Euglenophyceae. The highest phytoplankton abundance was observed in spring followed by early autumn, summer, and the lowest was in winter. The annual succession of Cyanophyceae was characterized by spring and early autumn period dominated by Microcystis sp. Anabaena sp. and Planktolymbya sp. with Microcystis sp. as the main blue-green algae represented. Chlorophyceae was characterized by rainy season domination of Chlorella vulgaris, Pediastrum sp. and Scenedesmus denticulatus with maximum abundance of Chlorella vulgaris. Whereas Bacillariophyceae was dominant during the winter period. Navicula angusta and Cyclotella meneghiniana were the most frequently occurring species of Bacillariophyceae throughout the study period. Euglenophyceae was dominant in late autumn and Euglena sp. was the dominant species. The effect of various physicochemical water quality parameters on the seasonal distribution and succession of the above mentioned phytoplankton population as well as the interaction and eutrophication are discussed.

Thiomer solidification of an ASR bottom ash: Optimization based on compressive strength and the characterization of heavy metal leaching

Son, Jae Hyop,Baek, Jin Woong,Choi, Angelo Earvin Sy,Park, Hung Suck ELSEVIER 2017 JOURNAL OF CLEANER PRODUCTION Vol.166 No.-

<P><B>Abstract</B></P> <P>This study examines the function of Thiomer solidification as a novel environment friendly construction material and its immobilization capacity over heavy metals in the automotive shredder residue (ASR) bottom ash. The morphology of the mixture using a field emission-scanning electron microscopy consistently illustrated the effective bonding between Thiomer and sand towards ASR bottom ash due to acting as fillers to reduce the gaps in its surface during Thiomer solidification. A D-optimal mixture design was further utilized in order to evaluate and optimize the parameters of Thiomer (25–35 wt%), ASR bottom ash (30–45 wt%) and sand (30–40 wt%) on the response of compressive strength. Result showed that optimum compressive strength of 55.9 MPa can be attained at 33.6, 36.4 and 30.0 wt% of Thiomer, ASR bottom ash and sand, respectively. The solidified Thiomer specimen showed superior structural strength over ordinary Portland cement concrete at curing time of 1 and 7 days. Furthermore, a mean heavy metal concentrations of 0.055 ppm Cu<SUP>2+</SUP>, 0.105 ppm Zn<SUP>2+</SUP>, 0.045 ppm Pb<SUP>2+</SUP>, 0.078 ppm Cr<SUP>6+</SUP> and 0.002 ppm Cd<SUP>2+</SUP> were achieved at various mixture designs in the heavy metal immobilization which satisfies stringent environmental standards. Thus, the application of Thiomer proves to be a promising construction material that can pose as an alternative over common cement due to promoting high durability and being eco-friendly.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel synthesis of Thiomer solidification in ASR bottom ash. </LI> <LI> Utilized D-optimal mixture design to determine optimum result for compressive strength. </LI> <LI> Thiomer solidification showed superior compressive strength over ordinary cement. </LI> <LI> Results indicate an efficient heavy metals immobilization by Thiomer solidification. </LI> <LI> Thiomer proves to be an innovative eco-friendly construction material. </LI> </UL> </P>

인공지능 안질환 진단 및 치료 기술동향 연구

조민호(MH Cho),백승운(SW Baek),김유리(YR Kim),한아름(AR Han),장혜정(HJ Jang),한태화(TH Han),장혁재(HJ Chang),송시영(SY Song) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.11

Research to use deep learning technology to assist in diagnostics is an important factor, and researchers are interested in new challenges much. By applying this deep learning technology, we analyzed cases of artificial intelligence research in the field of diagnosis and treatment of eye diseases.