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Mizan, Md Furkanur Rahaman,Ashrafudoulla, Md,Sadekuzzaman, Mohammad,Kang, Iksoon,Ha, Sang-Do Elsevier 2018 Food Control Vol.89 No.-
<P><B>Abstract</B></P> <P>The aim of this study was to evaluate the promotive and/or inhibitory effects of NaCl, glucose, their combinations on biofilm formation and quorum sensing (QS) autoinducer-2 (AI-2) production on black tiger shrimp surfaces, using three strains of <I>Vibrio parahaemolyticus</I>. Initially, six different NaCl concentrations (0.5, 1, 2, 3, 4, and 5%) were evaluated for a maximum biofilm formation (∼6.3 log CFU/cm<SUP>2</SUP>) at 30 °C. Inhibitory effects of glucose at six different levels (0.005, 0.01, 0.015, 0.02, 0.025, and 0.05%) were then investigated using the NaCl level previously found for a maximal biofilm formation. <I>V. parahaemolyticus</I> formed the best biofilm at 2% NaCl and the least biofilm at 5% NaCl, regardless of <I>vibrio</I> strain. In combination of 2% NaCl and glucose at different levels, the largest biofilm was observed at 0.015%, with the least biofilm seen at 0.05%, regardless of <I>vibrio</I> strain. Addition of salt and glucose more than the optimal level (2% salt and 0.015% glucose at 2% salt) inducted a stepwise inhibition of <I>vibrio</I> growth and biofilm formation in a continuous matter. In the visual evaluation, similar results were observed for <I>vibrio</I> growth, biofilm formation, live/dead cell detection, and quorum sensing.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Combined effect of NaCl-Glucose were studied on <I>V. parahaemolyticus</I> biofilm and quorum sensing. </LI> <LI> AI-2 detection was performed by HPLC-FLD. </LI> <LI> FESEM and CLSM analysis were investigated for visual observation. </LI> </UL> </P>