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Anondho Wijanarko,Dianursanti,Antonius Yudi Sendjaya,Heri Hermansyah,Arief Budi Witarto,Misri Gozan,Bondan Tiara Sofyan,Kazuhiro Asami,Kazuhisa Ohtaguchi,Roekmijati Widaningroem Soemantojo,송승구 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.4
Microalgae perform oxygenic photosynthesis and are capable of taking up a large amount of CO₂, using an inducible CO₂ concentrating mechanism (CCM), and fixing CO₂ into higher compounds. These characteristics make the microalgae potentially useful for removal and utilization of CO₂ emitted from industrial plants and, generally, the usage of photosynthetic microorganisms has increased and significantly improved as a solution for CO₂ emissions. In this light and based on previous research using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement was sought for CO₂fixation and biomass production by Chlorella vulgaris Buitenzorg by increasing the photon flux density concurrent with increases in culture biomass during the cellular growth phase and was compared to cultures of Chlorella grown at optimal constant illumination, with all cultures grown using Bennick basal medium, 29℃, and a flow of 1.0 atm. 10% CO₂ enriched air delivered to three in serial photobioreactors of 0.200 dm³capacity each. The results showed that increasing illumination during culture increased biomass production of Chlorella by ~60% as well as increased CO₂ fixation ability by ~7.0%. It was also demonstrated that the non-competitive inhibition of [HCO₃-] as a carbon source significantly affected the cultivation in both the increasing and constant photon flux density regimes.
Wijanarko Anondho,Dianursanti Dianursanti,Gozan Misri,Andika Sang Made Krisna,Widiastuti Paramita,Hermansyah Heri,Witarto Arief Budi,Asami Kazuhiro,Soemantojo Roekmijati Widaningroem,Ohtaguchi Kazuhis The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
Alteration of illumination with optimum carbon dioxide fixation-based curve in this research successfully enhanced the $CO_{2}-fixation\;(q_CO_{2}$ capability of Chlorella vulgaris Buitenzorg cultivated in a bubble column photo bioreactor. The level of $CO_{2}$ fixation was up to 1.91 times that observed from cultivation with intensification of illumination on an optimum growth-based curve. During 144 h of cultivation, alteration of light intensity on an optimum $CO_{2}-fixation-based$ curve produced a $q_CO_{2}$ of $12.8\;h^{-1}$. Meanwhile, alteration of light intensity with a growth-based curve only produced a $q_CO_{2}$ of $6.68\;h^{-1}$. Increases in light intensity based on a curve of optimum $CO_{2}-fixation$ produced a final cell concentration of about 5.78 g/L. Both cultivation methods were carried out under ambient pressure at a temperature of $29^{\circ}C$ with a superficial gas velocity of $2.4\;m/h(U_{G}$. Cells were grown on Beneck medium in a 1.0 L Bubble Column Photo bioreactor illuminated by a Phillips Halogen Lamp (20 W/12 V/50 Hz). The inlet gas had a carbon dioxide content of 10%.
Song Seung Koo,Anondho Wijanarko,Dianursanti,Misri Gozan,Sang Made Krisna Andika,Paramita Widiastuti,Heri Hermansyah,Arief Budi Witarto,Kazuhiro Asami,Roekmijati Widaningroem Soemantojo,Kazuhisa Ohtag 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
Alteration of illumination with optimum carbon dioxide fixation-based curve in this research successfully enhanced the CO2-fixation (qCO2) capability of Chlorella vulgaris Buitenzorg cultivated in a bubble column photo bioreactor. The level of CO2 fixation was up to 1.91 times that observed from cultivation with intensification of illumination on an optimum growth-based curve. During 144 h of cultivation, alteration of light intensity on an optimum CO2-fixation-based curve produced a qCO2 of 12.8 h1. Meanwhile, alteration of light intensity with a growth-based curve only produced a qCO2 of 6.68 h1. Increases in light intensity based on a curve of optimum CO2-fixation produced a final cell concentration of about 5.78 g/L. Both cultivation methods were carried out under ambient pressure at a temperature of 29oC with a superficial gas velocity of 2.4 m/h (UG). Cells were grown on Beneck medium in a 1.0 L Bubble Column Photo bioreactor illuminated by a Phillips Halogen Lamp (20 W/12 V/50 Hz). The inlet gas had a carbon dioxide content of 10%.